Effects of storage time and thawing methods on the recovery of Mycoplasma species in milk samples from cows with intramammary infections

Journal of Dairy Science

Volumn 87, Issue 4, 2004, Pages 933-936

  Biddle, M K ^a,b   Fox, L K ^a,b   Hancock, D D ^a,b   Gaskins, C T ^a,b   Evans, M A ^a,c  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b Department of Animal Science ^*

^c Program in Statistics   (United States)

Author keywords

Mycoplasma; Recovery; Storage; Thawing

Indexed keywords

BOS TAURUS; MYCOPLASMA;

ANIMAL; ARTICLE; BACTERIAL COUNT; CATTLE; CATTLE DISEASE; FEMALE; FOOD HANDLING; FREEZING; HEAT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MILK; MYCOPLASMA; PHYSIOLOGY; TIME;

ANIMALS; CATTLE; COLONY COUNT, MICROBIAL; FEMALE; FOOD HANDLING; FREEZING; HEAT; MASTITIS, BOVINE; MILK; MYCOPLASMA; TIME FACTORS;

EID: 4243050585     PISSN: 00220302     EISSN: None     Source Type: Journal    
DOI: 10.3168/jds.S0022-0302(04)73237-3     Document Type: Article

References (15)

1. Bashandy, E. Y., and L. E. Heider. 1979. The effect of freezing milk samples on the cultural results. Zentralbl. Veterinaermed. Reihe B. 26:1-6.
2. Biddle, M. K. 2002. Evaluation of Mycoplasma species shedding patterns in milk of lactating dairy cows with intramammary infections. M.S. Thesis, Washington State Univ., Pullman.
3. Hogan, J. S., R. N. Gonzalez, R. J. Harmon, S. C. Nickerson, S. P. Oliver, J. W. Pankey, and K. L. Smith. 1999. Pages 151-155 in National Mastitis Council Laboratory Handbook on Bovine Mastitis. National Mastitis Council, Madison, WI.
4. Kelton, W. 1964. Storage of mycoplasma strains. J. Bacteriol. 87:588-592.
5. Luedecke, L. O., T. L. Forster, K. Williams, and J. K. Hillers. 1971. Effect of freezing and storage at -20°C on survival of mastitis pathogens. J. Dairy Sci. 55:417-418.
6. Mazur, P. 1970. Cryobiology: The freezing of biological systems. Science 168:939-949.
7. Murdough, P. A., K. E. Deitz, and J. W. Pankey. 1996. Effects of freezing on the viability of nine pathogens from quarters with subclinical mastitis. J. Dairy Sci. 79:334-336.
8. National Mastitis Council. 1981. Pages 28-30 in Microbiological procedures for use in the diagnosis of bovine mastitis. 2nd ed. Carter Press, Inc., IA.
9. Pankey, J. W., J. K. Wadsworth, K. H. Metha, and P. A. Murdough. 1987. Effects of storage on viability of mastitis pathogens. J. Dairy Sci. 70(Suppl. 1):132. (Abstr.)
10. SAS/STAT User's Guide, Release 8.1.2001. SAS Inst, Inc., Cary, NC.
11. Schukken, Y. H., J. A. H. Smit, F. J. Grommers, D. Vandegeer, and A. Brand. 1989. Effect of freezing on bacteriologic culturing on mastitis milk samples. J. Dairy Sci. 72:1900-1906.
12. Storper, M., G. Ziv, and A. Saran. 1982. Effect of storing milk samples at -18°C on the viability of certain udder pathogens. Refuah Vet. 39:1-2.
13. Uchida, I. Y., and K. Hashimoto. 1987. Rapid identification of mycoplasma by indirect immunoperoxidase test using small square filter paper. J. Clin. Microbiol. 25:17-21.
14. Villanueva, M. R., J. W. Tyler, and M. C. Thurmond. 1991. Recovery of Streptococcus agalactiae and Staphylococcus aureus from fresh and frozen bovine milk. JAVMA 198:1398-1400.
15. Zar, J. H. 1996. Pages 272-274 in Biostatistical Analysis. 3rd ed. Prentice Hall, Englewood Cliffs, NJ.