Sequence determinants for the reaction specificity of murine (12R)-lipoxygenase: Targeted substrate modification and site-directed mutagenesis

Journal of Biological Chemistry

Volumn 280, Issue 44, 2005, Pages 36633-36641

  Meruvu, Sunitha ^a,b,d   Walther, Matthias ^a   Ivanov, Igor ^a   Hammarström, Sven ^b   Fürstenberger, Gerhard ^c   Krieg, Peter ^c   Reddanna, Pallu ^d   Kuhn, Hartmut ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b LINKÖPING UNIVERSITY   (Sweden)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^d UNIVERSITY OF HYDERABAD   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CARBOXYLIC ACIDS; ENZYME KINETICS; ESTERS; MUTAGENESIS; OXYGENATORS; REACTION KINETICS; STEREOCHEMISTRY;

ARACHIDONIC ACID; MAMMALIAN LIPOXYGENASE (LOX); MURINE; STEREOSELECTIVITY;

ENZYMES;

20 HYDROXYICOSATETRAENOIC ACID; 20 HYDROXYICOSATETRAENOIC ACID METHYL ESTER; ALANINE; AMINO ACID; ARACHIDONATE 12 LIPOXYGENASE; ARACHIDONATE 15 LIPOXYGENASE; ARACHIDONIC ACID; FATTY ACID; GLYCINE; HYDROXYICOSATETRAENOIC ACID; HYDROXYICOSATETRAENOIC ACID METHYL ESTER; ISOENZYME; ISOLEUCINE; LINOLEIC ACID; LINOLEIC ACID METHYL ESTER; PHENYLALANINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SEQUENCE; ARTICLE; CHIRALITY; ENANTIOSELECTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; MOUSE; MUTATION; NONHUMAN; OXYGENATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY;

12-HYDROXY-5,8,10,14-EICOSATETRAENOIC ACID; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARACHIDONATE 12-LIPOXYGENASE; BINDING SITES; HUMANS; HYDROXYEICOSATETRAENOIC ACIDS; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RABBITS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY; VALINE;

MAMMALIA; MURINAE; ORYCTOLAGUS CUNICULUS;

EID: 27744484756     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M508260200     Document Type: Article

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