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Volumn 1, Issue 8-9, 2005, Pages 844-848

Sensitive detection of proteins using difunctional DMA-gold nanoparticles

Author keywords

Analytical methods; DNA directed assembly; Nanoparticles; Proteins; Supramolecular chemistry

Indexed keywords

DNA; GOLD; IMMUNOLOGY; NANOSTRUCTURED MATERIALS; OLIGOMERS; SUPRAMOLECULAR CHEMISTRY;

EID: 27744445693     PISSN: 16136810     EISSN: 16136829     Source Type: Journal    
DOI: 10.1002/smll.200500063     Document Type: Article
Times cited : (74)

References (29)
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    • AuNPs modified with aptamers or ribozymes have been used to detect adenosin (J. Liu, Y. Lu, Anal. Chem. 2004, 76, 1627-1632)
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    • Liu, J.1    Lu, Y.2
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    • or to determine metal ions (J. Liu, Y. Lu, J. Am. Chem. Soc. 2004, 126, 12298-12305).
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    • Liu, J.1    Lu, Y.2
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    • [6] Based on these results, one can estimate that about 250 oligonucleotides of each kind are attached to the particle's surface (i.e., a total number of about 500 oligomers per 40 nm AuNP). The maximum binding efficiency of the antibody-DNA conjugate 7 was determined to be about 32-64 equivalents (Figure S1 in the Supporting Information), which suggests that about 32-64 antibody molecules are attached to the AuNP. Moreover, these data indicate a hybridization efficiency of conjugate 7 of about 13-25%. These numbers are in agreement with hybridization efficiency data reported by others (L. M. Demers, C. A. Mirkin, R. C. Mucic, R. A. Reynolds, R. L. Letsinger, R. Elghanian, G. Viswanadham, Anal. Chem. 2000, 72, 5535-5541.
    • (2000) Anal. Chem. , vol.72 , pp. 5535-5541
    • Demers, L.M.1    Mirkin, C.A.2    Mucic, R.C.3    Reynolds, R.A.4    Letsinger, R.L.5    Elghanian, R.6    Viswanadham, G.7


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.