DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 588, Issue 1, 2005, Pages 1-6

  Iwakawa, Mayumi ^a   Goto, Miyako ^a   Noda, Shuhei ^a   Sagara, Masashi ^a   Yamada, Shigeru ^a   Yamamoto, Naoto ^b   Kawakami, Yoshihiro ^c   Matsui, Yoshifumi ^d   Miyazawa, Yukimasa ^e   Yamazaki, Hideya ^f   Tsuji, Hiroshi ^a   Ohno, Tatsuya ^a   Mizoe, Junetsu ^a   Tsujii, Hirohiko ^a   Imai, Takashi ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^b CHIBA CANCER CENTER   (Japan)

^c Kawakami Breast Thyroid Clinic   (Japan)

^d Shimizu Kosei General Hospital   (Japan)

^e CHIBA UNIVERSITY   (Japan)

^f TOYONAKA MUNICIPAL HOSPITAL   (Japan)

Author keywords

Alkaline comet assay; Automated analysis; Cancer patients; Radiotherapy

Indexed keywords

ARTICLE; BLOOD CELL; BLOOD DONOR; BREAST CANCER; CANCER; CANCER PATIENT; CELL LINE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; EPSTEIN BARR VIRUS; ESOPHAGUS CANCER; HEAD CANCER; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LUNG CANCER; LYMPHOBLASTOID CELL LINE; MAJOR CLINICAL STUDY; NECK CANCER; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; RADIOSENSITIVITY; UTERINE CERVIX CANCER; VIRUS CELL TRANSFORMATION; VOLUNTEER; X IRRADIATION;

BLOOD DONORS; CELL LINE, TRANSFORMED; COMET ASSAY; DNA DAMAGE; DNA REPAIR; FEMALE; HERPESVIRUS 4, HUMAN; HUMANS; LEUKOCYTES, MONONUCLEAR; NEOPLASMS; RADIATION TOLERANCE;

CANCER VIRUS; HUMAN HERPESVIRUS 4;

EID: 27744438940     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2005.07.012     Document Type: Article

References (23)

1. R.J. Albertini, D. Anderson, G.R. Douglas, L. Hagmar, K. Hemminki, F. Merlo, A.T. Natarajan, H. Norppa, D.E. Shuker, R. Tice, M.D. Waters, and A. Aitio IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans, International Programme on Chemical Safety Mutat. Res. 463 2000 111 172
2. O. Ostling, and K.J. Johanson Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells Biochem. Biophys Res. Commun. 123 1984 291 298
3. R.R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J.C. Ryu, and Y.F. Sasaki Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing Environ. Mol. Mutagenesis 35 2000 206 221
4. F. Faust, F. Kassie, S. Knasmuller, R.H. Boedecker, M. Mann, and V. Mersch-Sundermann The use of the alkaline comet assay with lymphocytes in human biomonitoring studies Mutat. Res. 566 2004 209 229
5. S. Colleu-Durel, N. Guitton, K. Nourgalieva, F. Legue, J. Leveque, B. Danic, and C. Chenal Alkaline single-cell gel electrophoresis (comet assay): a simple technique to show genomic instability in sporadic breast cancer Eur. J. Cancer 40 2004 445 451
6. M. Morocz, J. Kalman, A. Juhasz, I. Sinko, A.P. McGlynn, C.S. Downes, Z. Janka, and I. Rasko Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer's disease Neurobiol. Aging 23 2002 47 53
7. G. Speit, T. Witton-Davies, W. Heepchantree, K. Trenz, and H. Hoffmann Investigations on the effect of cigarette smoking in the comet assay Mutat. Res. 542 2003 33 42
8. V.A. Tronov, E.M. Konstantinov, E. Petrakou, S. Tsilimigaki, and S.M. Piperakis Nicotinamide "protects" resting lymphocytes exposed to hydrogen peroxide from necrosis but not from apoptosis Cell Biol. Toxicol. 18 2002 359 367
9. M.A. Moneef, B.T. Sherwood, K.J. Bowman, R.C. Kockelbergh, R.P. Symonds, W.P. Steward, J.K. Mellon, and G.D. Jones Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity Brit. J. Cancer 89 2003 2271 2276
10. T.R. Smith, M.S. Miller, K.K. Lohman, L.D. Case, and J.J. Hu DNA damage and breast cancer risk Carcinogenesis 24 2003 883 889
11. K. Trenz, A. Rothfuss, P. Schutz, and G. Speit Mutagen sensitivity of peripheral blood from women carrying a BRCAl or BRCA2 mutation Mutat. Res. 500 2002 89 96
12. A. Vral, H. Louagie, H. Thierens, J. Philippe, M. Cornelissen, and L. de Ridder Micronucleus frequencies in cytokinesis-blocked human B lymphocytes after low dose gamma-irradiation Int. J. Radiat. Biol. 73 1998 549 555
13. A. Vral, H. Thierens, P. Bryant, and L. De Ridder A higher micronucleus yield in B- versus T-cells after low-dose gamma-irradiation is not linked with defective Ku86 protein Int. J. Radiat. Biol. 77 2001 329 339
14. A. Baeyens, H. Thierens, K. Vandenbulcke, L. De Ridder, and A. Vral The use of EBV-transformed cell lines of breast cancer patients to measure chromosomal radiosensitivity Mutagenesis 19 2004 285 290
15. Ministry of Education, Culture, Sports, Science and Technology; Ministry of Health, Labor and Welfare; and Ministry of Economy, Trade and Industry, Ethics Guidelines for Human Genome/Gene Analysis Research 2001. available at http://www.mext.go.jp/amenu/shinkou/seimei/genomeshishin/html/shis hin/rinrishishinenglish.pdf.
16. C. Mayer, O. Popanda, O. Zelezny, M.C. von Brevern, A. Bach, H. Bartsch, and P. Schmezer DNA repair capacity after gamma-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes DNA Repair 1 2002 237 250
17. W. Bocker, T. Bauch, W.U. Muller, and C. Streffer Image analysis of comet assay measurements Int. J. Radiat. Biol. 72 1997 449 460
18. P.L. Olive, J.P. Banath, and R.E. Durand Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay Radiat. Res. 122 1990 86 94
19. C. Schunck, T. Johannes, D. Varga, T. Lorch, and A. Plesch New developments in automated cytogenetic imaging: unattended scoring of dicentric chromosomes, micronuclei, single cell gel electrophoresis, and fluorescence signals Cytogenet. Genome Res. 104 2004 383 389
20. M. Berwick, and P. Vineis Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review J. Natl. Cancer Inst. 92 2000 874 897
21. S. Colleu-Durel, N. Guitton, K. Nourgalieva, J. Leveque, B. Danic, and C. Chenal Genomic instability and breast cancer Oncol. Res. 8 2001 1001 1005
22. N. Rajaee-Behbahani, P. Schmezer, A. Risch, W. Rittgen, K.W. Kayser, H. Dienemann, V. Schulz, P. Drings, S. Thiel, and H. Bartsch Altered DNA repair capacity and bleomycin sensitivity as risk markers for non-small cell lung cancer Int. J. Cancer. 95 2001 86 91
23. M.B. Schabath, M.R. Spitz, H.B. Grossman, K. Zhang, C.P. Dinney, P.J. Zheng, and X. Wu Genetic instability in bladder cancer assessed by the comet assay J. Natl. Cancer Inst. 95 2003 540 547