A highly efficient preparative-scale generic immunoaffinity chromatography method for the purification of antibodies to hydrophobic haptens: Purification procedure and monitoring tests

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 827, Issue 2, 2005, Pages 182-192

  Griffith, Hannah M T ^a   Abuknesha, Ramadan A ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Generic elution methods; Hydrophobic haptens; Immunoaffinity chromatography

Indexed keywords

ANTIBODIES; BIOASSAY; GELS; HORMONES; HYDROPHILICITY; IMMUNOLOGY; MOLECULAR STRUCTURE; PURIFICATION;

ASSAY DETECTION; GENERIC ELUTION METHODS; HYDROPHOBIC HAPTENS; IMMUNOAFFINITY CHROMATOGRAPHY;

AFFINITY CHROMATOGRAPHY;

2 TERT BUTYLAMINO 4 CYCLOPROPYLAMINO 6 METHYLTHIO 1,3,5 TRIAZINE; ACETONITRILE; AZAMETHONIUM; ESTROGEN; HAPTEN; IMMUNOGLOBULIN G ANTIBODY; PENTACHLOROPHENOL; POLYCLONAL ANTIBODY; PROPIONIC ACID; SEPHAROSE;

ANTIBODY ISOLATION; ANTIGEN BINDING; ARTICLE; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL; HYDROPHILICITY; HYDROPHOBICITY; IMMUNOAFFINITY CHROMATOGRAPHY; MONITORING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PURIFICATION; SOLID STATE;

ANIMALS; ANTIBODIES; ANTIBODY SPECIFICITY; CHROMATOGRAPHY, AFFINITY; CROSS REACTIONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESTRADIOL; HAPTENS; HYDROPHOBICITY; PENTACHLOROPHENOL; SHEEP; TRIAZINES;

EID: 27644482193     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2005.08.018     Document Type: Article

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