Strategy for fractionating high-affinity antibodies to steroid hormones by affinity chromatography

Analyst

Volumn 121, Issue 7, 1996, Pages 939-944

  Giraudi, Gianfranco ^a   Baggiani, Claudio ^a  

^a Department of Analytical Chemistry ^*  (Italy)

Author keywords

Affinity chromatography; Affinity constants; Antiserum fractionation; Cross reaction; Dioxane; Dissociation rate constants; Immunoaffinity; pH dependence; Solvent effect; Testosterone antiserum

Indexed keywords

ANTISERUM; BOVINE SERUM ALBUMIN; DIOXANE; HORMONE ANTIBODY; SEPHAROSE; TESTOSTERONE;

AFFINITY CHROMATOGRAPHY; ANTIBODY AFFINITY; ARTICLE; FRACTIONATION; PH;

EID: 0030016764     PISSN: 00032654     EISSN: None     Source Type: Journal    
DOI: 10.1039/an9962100939     Document Type: Article

References (18)

1. Bouzerna, N., Hammadi, H., Richard, C., Formstecher, P., and Dautrevaux, M., J. Immunol. Methods, 1988, 112, 251.
2. Massaglia, A., Rolleri, E., Barbieri, U., and Rosa, U., J. Clin. Endocrinol. Metab., 1974, 38, 820.
3. Formstecher, P., Hammadi, H., Bouzerna, N., and Dautrevaux, M., J. Chromatogr., 1986, 369, 379.
4. Grenot, C., and Cuilleron, C. Y., Steroids, 1979, 34, 2261.
5. Müller, R. F., Palluk, R., and Kemple, M. A., J. Chem. Technol. Biotechnol., 1984, 34B, 263.
6. Lewis, J. G., and Elder, P. A., J. Steroid Biochem., 1985, 22, 713.
7. Podestà, A., Luisi, M., and Montagnoli, G., J. Steroid Biochem. Mol. Biol., 1991, 39, 63.
8. Parini, C., Corocher, N., Bacigalupo, M. A., Baggiani, C., Colombi, S., and Giraudi, G., Analyst, 1995, 120, 1153.
9. Giraudi, G., and Petrarulo, M., Ann. Chim. (Rome), 1989, 79, 231.
10. Giraudi, G., Baggiani, C., and Costa, L., Ann. Chim. (Rome), 1990, 80, 111.
11. Giraudi, G., Cenderelli, G., and Patrito, G., Steroids, 1983, 42, 475.
12. Scatchard, G., Ann. N. Y. Acad. Sci., 1949, 51, 660.
13. Abraham, G. E., J. Clin. Endocrinol. Metab., 1969, 29, 866.
14. Janosky, C., Shulman, F. C., and Wight, G. E., Steroids, 1973, 23, 49.
15. Affinity Chromatography, Principle and Methods, Pharmacia LKB, Uppsala, 1978, pp. 16-30.
16. Skubitz, K. M., and Smith, T. W., J. Immunol., 1975, 114, 1369.
17. Giraudi, G., and Baggiani, C., Biochim. Biophys. Acta, 1993, 1157, 211.
18. Abercrombie, D. M., and Chaiken, I. M., in Affinity Chromatography: a Practical Approach, ed. Dean, P. D. G., Johnson, W. S., and Middle, F. A., IRL Press, Oxford, 1985, pp. 173-5.