Promoter-dependent disruption of genes: Simple, rapid, and specific PCR-based method with application to three different yeast

Current Genetics

Volumn 48, Issue 2, 2005, Pages 117-125

  Edlind, Thomas D ^a   Henry, Karl W ^a   Vermitsky, John Paul ^a   Edlind, Merritt P ^a   Raj, Shriya ^a   Katiyar, Santosh K ^a  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Candida albicans; Candida glabrata; Genedeletion; Saccharomyces cerevisiae; Transformation

Indexed keywords

CYCLOHEXIMIDE; FLUOROOROTIC ACID; MILTEFOSINE;

5' UNTRANSLATED REGION; ALLELE; ARTICLE; CANDIDA ALBICANS; CANDIDA GLABRATA; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS MUTANT; GENE DISRUPTION; GENE EXPRESSION; GENE TARGETING; GROWTH RATE; MARKER GENE; MOULD; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYADENYLATION; POLYMERASE CHAIN REACTION; PREDICTION; PRIORITY JOURNAL; PROMOTER REGION; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION; YEAST;

CANDIDA ALBICANS; CANDIDA GLABRATA; GENE TARGETING; METHODS; MUTATION; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; SACCHAROMYCES CEREVISIAE; YEASTS;

CANDIDA ALBICANS; CANDIDA GLABRATA; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 27644475881     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-005-0008-3     Document Type: Article

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