Production, purification and characterisation of recombinant Fahsin, a novel antistasin-type proteinase inhibitor

FEMS Yeast Research

Volumn 5, Issue 11, 2005, Pages 1069-1077

  De Bruin, Eric C ^a,b   Roem, Dorina ^a,b   Bulder, Ingrid ^a,b   Dieker, Miranda ^a,b   Voerman, Gerard ^c   Hack, C Erik ^a,b,d  

^a SANQUIN RESEARCH   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c VitaLeech Bioscience NV   (Netherlands)

^d VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Fahsin; Human neutrophil elastase; Inflammation; Leech; Pichia pastoris

Indexed keywords

ANTISTASIN; BDELLASTASIN; FAHSIN; FREE RADICAL; GHILANTEN; GUAMERIN; HIRUSTASIN; LEUKOCYTE ELASTASE INHIBITOR; MYELOPEROXIDASE; OXYGEN RADICAL; PIGUAMERIN; SYNTHETIC DNA; THEROSTASIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG BINDING; DRUG PURIFICATION; DRUG SENSITIVITY; DRUG SPECIFICITY; DRUG SYNTHESIS; ENZYME INHIBITION; ENZYME KINETICS; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; LEECH; MASS SPECTROMETRY; NEUTROPHIL; NONHUMAN; NONVIRAL GENE THERAPY; OXIDATION; PICHIA PASTORIS; QUANTUM YIELD; RECOMBINANT GENE;

ANIMALS; INVERTEBRATE HORMONES; LEECHES; PICHIA; PROTEASE INHIBITORS; RECOMBINANT FUSION PROTEINS; SERINE PROTEINASE INHIBITORS;

HIRUDINIDA; LIMNATIS NILOTICA; PICHIA PASTORIS;

EID: 27644439961     PISSN: 15671356     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsyr.2005.03.005     Document Type: Article

References (33)

1. H.I. Jung, S.I. Kim, K.S. Ha, C.O. Joe, and K.W. Kang Isolation and characterization of guamerin, a new human leukocyte elastase inhibitor from Hirudo nipponia J. Biol. Chem. 270 1995 13879 13884
2. D.R. Kim, and K.W. Kang Amino acid sequence of piguamerin, an antistasin-type protease inhibitor from the blood-sucking leech Hirudo nipponia Eur. J. Biochem. 254 1998 692 697
3. C. Sollner, R. Mentele, C. Eckerskorn, H. Fritz, and C.P. Sommerhoff Isolation and characterization of hirustasin, an antistasin-type serine-proteinase inhibitor from the medical leech Hirudo medicinalis Eur. J. Biochem. 219 1994 937 943
4. M. Moser, E. Auerswald, R. Mentele, C. Eckerskorn, H. Fritz, and E. Fink Bdellastasin, a serine protease inhibitor of the antistasin family from the medical leech (Hirudo medicinalis) - primary structure, expression in yeast, and characterisation of native and recombinant inhibitor Eur. J. Biochem. 253 1998 212 220
5. P.R. Mittl, S. Di Marco, G. Fendrich, G. Pohlig, J. Heim, C. Sommerhoff, H. Fritz, J.P. Priestle, and M.G. Grutter A new structural class of serine protease inhibitors revealed by the structure of the hirustasin-kallekrein complex Structure 5 1997 253 264
6. T.J. Moraes, C.W. Chow, and G.P. Downey Proteases and lung injury Crit. Care Med. 31 2003 S189 S194
7. J. Fricker Elastase inhibitor for detection and treatment of inflammation and infection Mol. Med. Today 4 1998 5
8. P.S. Hiemstra Novel roles of protease inhibitors in infection and inflammation Biochem. Soc. Trans. 30 2002 116 120
9. S.D. Shapiro Neutrophil elastase: path clearer, pathogen killer, or just pathologic Am. J. Respir. Cell. Mol. Biol. 26 2002 266 268
10. V.J. Uitto, C.M. Overall, and C. McCulloch Proteolytic host cell enzymes in gingival crevice fluid Periodontology 31 2003 77 104
11. M. Salzet, D. Vieau, and G.B. Stefano Serpins: an evolutionarily conserved survival strategy Immunol. Today 20 1999 541 544
12. D. Johnson, and J. Travis The oxidative inactivation of human α-1-proteinase inhibitor. Further evidence for methionine at the reactive center J. Biol. Chem. 254 1979 4022 4026
13. M. Padrines, M. Schneider-Pozzer, and J.G. Bieth Inhibition of neutrophil elastase by α-1-proteinase inhibitor oxidized by activated neutrophils Am. Rev. Respir. Dis. 139 1989 783 790
14. J. Duranton, and J.G. Bieth Inhibition of proteinase 3 by α1-antitrypsin in vitro predicts very fast inhibition in vivo Am. J. Respir. Cell. Mol. Biol. 29 2003 57 61
15. Voerman, G. (1996) A novel family of protease inhibitors and other biologically active substances. Pat. Appl. WO 9613585.
16. S.N. Ho, H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease Site-directed mutagenesis by overlap extension using the polymerase chain reaction Gene 77 1989 51 59
17. J.M. Cregg, K.J. Barringer, A.Y. Hessler, and K.R. Madden Pichia pastoris as a host system for transformations Mol. Cell. Biol. 5 1985 3376 3385
18. D.M. Becker, and L. Guarente High-efficiency transformation of yeast by electroporation Methods Enzymol. 194 1991 182 187
19. K.J. Potter, W. Zhang, L.A. Smith, and M.M. Meagher Production and purification of the heavy chain fragment C of botulinum neurotoxin, serotype A, expressed in the methylotrophic yeast Pichia pastoris Protein Expr. Purif. 19 2000 393 402
20. M.W.T. Werten, T.J. van den Bosch, R.D. Wind, H. Mooibroek, and F.A. de Wolf High-yield secretion of recombinant gelatins by Pichia pastoris Yeast 15 1999 1087 1096
21. H.R. Ellis, and L.B. Poole Novel application of 7-chloro-4-nitrobenzo-2- oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl hydroperoxide reductase Biochemistry 36 1997 15013 15018
22. C. Boudier, and J.G. Bieth Oxidized mucus proteinase inhibitor: a fairly potent neutrophil elastase inhibitor Biochem. J. 303 1994 61 68
23. M. Dixon The determination of enzyme inhibitor constants Biochem. J. 55 1953 170 171
24. J.H. Nuijens, J.J. Abbink, Y.T. Wachtfogel, R.W. Colman, A.J. Eerenberg, D. Dors, A.J. Kamp, R.J. Strack van Schijndel, L.G. Thijs, and C.E. Hack Plasma elastase α1-antitrypsin and lactoferrin in sepsis: evidence for neutrophils as mediators in fatal sepsis J. Lab. Clin. Med. 119 1992 159 168
25. C. Dunwiddie, N.A. Thornberry, H.G. Bull, M. Sardana, P.A. Friedman, J.W. Jacobs, and E. Simpson Antistasin, a leech-derived inhibitor of factor Xa. Kinetic analysis of enzyme inhibition and identification of the reactive site J. Biol. Chem. 264 1989 16694 16699
26. D.T. Blankenship, R.G. Brankamp, G.D. Manley, and A.D. Cardin Amino acid sequence of ghilanten: anticoagulant-antimetastatic principle of the South American leech, Haementeria ghilianii Biochem. Biophys. Res. Commun. 166 1990 1384 1389
27. V. Chopin, M. Salzet, J. Baert, F. Vandenbulcke, P.E. Sautiere, J.P. Kerckaert, and J. Malecha Therostasin, a novel clotting factor Xa inhibitor from the rhynchobdellid leech, Theromyzon tessulatum J. Biol. Chem. 275 2000 32701 32707
28. F. Markwardt Hirudin as alternative anticoagulant - a historical review Semin. Thromb. Hemost. 28 2002 405 414
29. S.A. Rosenfeld, D. Nadeau, J. Tirado, G.F. Hollis, R.M. Knabb, and S. Jia Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris Protein Expr. Purif. 8 1996 476 482
30. S. Di Marco, G. Fendrich, R. Knecht, A. Strauss, G. Pohlig, J. Heim, J.P. Priestle, C.P. Sommerhoff, and M.G. Grutter Recombinant hirustasin: production in yeast, crystallization, and interaction with serine proteases Protein Sci. 6 1997 109 118
31. K.Y. Kim, H.K. Lim, K.J. Lee, D.H. Park, K.W. Kang, S.I. Chung, and K.H. Jung Production and characterization of recombinant guamerin, an elastase-specific inhibitor, in the methylotrophic yeast Pichia pastoris Protein Expr. Purif. 20 2000 1 9
32. C.T. Przysiecki, J.G. Joyce, P.M. Keller, H.Z. Markus, C.E. Carty, A. Hagopian, M.K. Sardana, C.T. Dunwiddie, R.W. Ellis, and W.J. Miller Characterization of recombinant antistasin secreted by Saccharomyces cerevisiae Protein Expr. Purif. 3 1992 185 195
33. R.G. Brankamp, K. Sreekrishna, P.L. Smith, D.T. Blankenship, and A.D. Cardin Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast Pichia pastoris Protein Expr. Purif. 6 1995 813 820