Overexpression of the M2-2 protein of respiratory syncytial virus inhibits viral replication

Journal of Virology

Volumn 79, Issue 22, 2005, Pages 13943-13952

  Cheng, Xing ^a   Park, HyunJung ^a   Zhou, Helen ^a   Jin, Hong ^a  

^a MEDIMMUNE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS PROTEIN M2 2;

AMINO TERMINAL SEQUENCE; ARTICLE; CODON; GENE; GENE OVEREXPRESSION; GENE SEQUENCE; INHIBITION KINETICS; M2 1 GENE; M2 2 GENE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; SEQUENCE ANALYSIS; TRANSCRIPTION REGULATION; VIRUS INHIBITION; VIRUS REPLICATION;

ANIMALS; BASE SEQUENCE; CELL LINE; CERCOPITHECUS AETHIOPS; DNA, COMPLEMENTARY; GENE AMPLIFICATION; GENE EXPRESSION REGULATION, VIRAL; GENES, VIRAL; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RESPIRATORY SYNCYTIAL VIRUSES; RESTRICTION MAPPING; RNA, VIRAL; VERO CELLS; VIRAL PROTEINS; VIRUS REPLICATION;

RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS;

EID: 27644439478     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.79.22.13943-13952.2005     Document Type: Article

References (41)

1. Abraham, G., and A. K. Banerjee. 1976. Sequential transcription of the genes of vesicular stomatitis virus. Proc. Natl. Acad. Sci. USA 73:1504-1508.
2. Ahmadian, G., P. Chambers, and A. J. Easton. 1999. Detection and characterization of proteins encoded by the second ORF of the M2 gene of pneumoviruses. J. Gen. Virol. 80:2011-2016.
3. Ahmadian, G., J. S. Randhawa, and A. J. Easton. 2000. Expression of the ORF-2 protein of the human respiratory syncytial M2 gene is initiated by a ribosomal termination-dependent reinitiation mechanism. EMBO J. 19: 2681-2689.
4. Ball, L. A., and C. N. White. 1976. Order of transcription of genes of vesicular stomatitis virus. Proc. Natl. Acad. Sci. USA 73:442-446.
5. Barik, S. 1992. Transcription of human respiratory syncytial virus genome RNA in vitro: requirement of cellular factor(s). J. Virol. 66:6813-6818.
6. Bermingham, A., and P. L. Collins. 1999. The M2-2 protein of human respiratory syncytial virus is a regulatory factor involved in the balance between RNA replication and transcription. Proc. Natl. Acad. Sci. USA 96:11259-11264.
7. Bilsel, P. A., and S. T. Nichol. 1990. Polymerase errors accumulating during natural evolution of the glycoprotein gene of vesicular stomatitis virus Indiana serotype isolates. J. Virol. 64:4873-4883.
8. Bossert, B., S. Marozin, and K. K. Conzelmann. 2003. Nonstructural proteins NS1 and NS2 of bovine respiratory syncytial virus block activation of interferon regulatory factor 3. J. Virol. 77:8661-8668.
9. Buchholz, U. J., S. Finke, and K. K. Conzelmann. 1999. Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J. Virol. 73:251-259.
10. Cheng, X., M. Munoz, H. Zhou, and H. Jin. 2002. Expression of beta-galactosidase by recombinant respiratory syncytial viruses for microneutralization assay. J. Virol. Methods 105:287-295.
11. Cheng, X., H. Zhou, R. S. Tang, M. G. Munoz, and H. Jin. 2001. Chimeric subgroup A respiratory syncytial virus with the glycoproteins substituted by those of subgroup B and RSV without the M2-2 gene are attenuated in African green monkeys. Virology 283:59-68.
12. Collins P. L., R. M. Chanock, and B. R. Murphy. 2001. Respiratory syncytial virus, p. 1443-1485. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.
13. Collins, P. L., M. G. Hill, J. Cristina, and H. Grosfeld. 1996. Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proc. Natl. Acad. Sci. USA 93:81-85.
14. Collins, P. L., M. G. Hill, and P. R. Johnson. 1990. The two open reading frames of the 22K mRNA of human respiratory syncytial virus: sequence comparison of antigenic subgroups A and B and expression in vitro. J. Gen. Virol. 71:3015-3020.
15. Collins, P. L., and B. R. Murphy. 2002. Respiratory syncytial virus: reverse genetics and vaccine strategies. Virology 296:204-211.
16. Collins, P. L., and G. W. Wertz. 1983. cDNA cloning and transcriptional mapping of nine polyadenylylated RNAs encoded by the genome of human respiratory syncytial virus. Proc. Natl. Acad. Sci. USA 80:3208-3212.
17. Collins, P. L., and G. W. Wertz. 1985. The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript. J. Virol. 54:65-71.
18. Cunningham, P. R., C. J. Weitzmann, and J. Ofengand. 1991. SP6 RNA polymerase stutters when initiating from an AAA ... sequence. Nucleic Acids Res. 19:4669-4673.
19. Fearns, R., and P. L. Collins. 1999. Model for polymerase access to the overlapped L gene of respiratory syncytial virus. J. Virol. 73:388-397.
20. Fearns, R., and P. L. Collins. 1999. Role of the M2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. J. Virol. 73:5852-5864.
21. Garcia-Barreno, B., A. Portela, T. Delgado, J. A. Lopez, and J. A. Melero. 1990. Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus. EMBO J. 9:4181-4187.
22. Grosfeld, H., M. G. Hill, and P. L. Collins. 1995. RNA replication by respiratory syncytial virus (RSV) is directed by the N, P, and L protein; transcription also occurs under these conditions but requires RSV superinfection for efficient synthesis of full-length mRNA. J. Virol. 69:5677-5686.
23. Hardy, R. W., S. B. Harmon, and G. W. Wertz. 1999. Diverse gene junctions of respiratory syncytial virus modulate the efficiency of transcription termination and respond differently to M2-mediated antitermination. J. Virol. 73:170-176.
24. Harmon, S. B., A. G. Megaw, and G. W. Wertz. 2001. RNA sequences involved in transcriptional termination of respiratory syncytial virus. J. Virol. 75:36-44.
25. Huang, Y. T., P. L. Collins, and G. W. Wertz. 1985. Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Res. 2:157-173.
26. Hubenthal-Voss, J., R. A. Houghten, L. Pereira, and B. Roizman. 1988. Mapping of functional and antigenic domains of the alpha 4 protein of herpes simplex virus 1. J. Virol. 62:454-462.
27. Jin, H., X. Cheng, H. Z. Zhou, S. Li, and A. Seddiqui. 2000. Respiratory syncytial virus that lacks open reading frame 2 of the M2 gene (M2-2) has altered growth characteristics and is attenuated in rodents. J. Virol. 74: 74-82.
28. Jin, H., D. Clarke, Z.-Y.H. Zhou, et al. 1998. Recombinant human respiratory syncytial virus (RSV) from cDNA and construction of subgroup A and B chimeric RSV. Virology 251:206-214.
29. Jin, H., B. Lu, H. Zhou, et al. 2003. Multiple amino acid residues confer temperature sensitivity to human influenza virus vaccine strains (FluMist) derived from cold-adapted A/Ann. Arbor/6/60. Virology 306:18-24.
30. Jin, H., H. Zhou, X. Cheng, R. Tang, M. Munoz, and N. Nguyen. 2000. Recombinant respiratory syncytial viruses with deletions in the NS1, NS2, SH, and M2-2 genes are attenuated in vitro and in vivo. Virology 273: 210-218.
31. Kuo, L., H. Grosfeld, J. Cristina, M. G. Hill, and P. L. Collins. 1996. Effects of mutations in the gene-start and gene-end sequence motifs on transcription of monocistronic and dicistronic minigenomes of respiratory syncytial virus. J. Virol. 70:6892-6901.
32. Moudy, R. M., W. M. Sullender, and G. W. Wertz. 2004. Variations in intergenic region sequences of human respiratory syncytial virus clinical isolates: analysis of effects on transcriptional regulation. Virology 327: 121-133.
33. Reimers, K., K. Buchholz, and H. Werchau. 2005. Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B. Virology 331:260-268.
34. Schlender, J., B. Bossert, U. Buchholz, and K. K. Conzelmann. 2000. Bovine respiratory syncytial virus nonstructural proteins NS1 and NS2 cooperatively antagonize alpha/beta interferon-induced antiviral response. J. Virol. 74: 8234-8242.
35. Spann, K. M., K. C. Tran, B. Chi, R. L. Rabin, and P. L. Collins. 2004. Suppression of the induction of alpha, beta, and lambda interferons by the NS1 and NS2 proteins of human respiratory syncytial virus in human epithelial cells and macrophages. J. Virol. 78:4363-4369.
36. Sutherland, K. A., P. L. Collins, and M. E. Peeples. 2001. Synergistic effects of gene-end signal mutations and the M2-1 protein on transcription termination by respiratory syncytial virus. Virology 288:295-307.
37. Tang, R. S., N. Nguyen, X. Cheng, and H. Jin. 2001. Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein for protein function and virus viability. J. Virol. 75:11328-11335.
38. Teng, M. N., and P. L. Collins. 1998. Identification of the respiratory syncytial virus proteins required for formation and passage of helper-dependent infectious particles. J. Virol. 72:5707-5716.
39. Teng, M. N., and P. L. Collins. 1999. Altered growth characteristics of recombinant respiratory syncytial viruses which do not produce NS2 protein. J. Virol. 73:466-473.
40. Teng, M. N., S. S. Whitehead, A. Bermingham, M. St. Claire, W. R. Elkins, B. R. Murphy, and P. L. Collins. 2000. Recombinant respiratory syncytial virus that does not express the NS1 or M2-2 protein is highly attenuated and immunogenic in chimpanzees. J. Virol. 74:9317-9321.
41. Yu, Q., R. W. Hardy, and G. W. Wertz. 1995. Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L proteins support replication of RS virus genomic RNA analogs and define minimal trans-acting requirements for RNA replication. J. Virol. 69:2412-2419.