Probing the role of Tyr 64 of Treponema denticola cystalysin by site-directed mutagenesis and kinetic studies

Biochemistry

Volumn 44, Issue 42, 2005, Pages 13970-13980

  Cellini, Barbara ^a   Bertoldi, Mariarita ^a   Montioli, Riccardo ^a   Voltattorni, Carla Borri ^a,b  

^a UNIVERSITY OF VERONA   (Italy)

^b Istituto di Chimica   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; CATALYSIS; ENZYMES; HYDROGEN BONDS; QUENCHING; SPECTROSCOPIC ANALYSIS;

ALDAMINE; CHEMICAL QUENCH EXPERIMENTS; RACEMIZATION;

MUTAGENESIS;

3 CHLOROALANINE; ALANINE; ALDAMINE; AMINE; BACTERIAL ENZYME; CYSTALYSIN; DEHYDROALANINE; ENAMINE; ENOLIMINE; IMINE; KETOENAMINE; MUTANT PROTEIN; PHOSPHATE; PYRIDOXAL 5 PHOSPHATE; PYRUVIC ACID; SCHIFF BASE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; COENZYME; ELIMINATION REACTION; ENZYME KINETICS; ENZYME STRUCTURE; FLUORESCENCE; HYDROGEN BOND; NONHUMAN; PH; PRIORITY JOURNAL; RACEMIZATION; SITE DIRECTED MUTAGENESIS; SPECTRAL SENSITIVITY; TITRIMETRY; TREPONEMA DENTICOLA; WILD TYPE;

ANIMALS; BASE SEQUENCE; CYSTATHIONINE GAMMA-LYASE; DNA PRIMERS; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PYRIDOXAL PHOSPHATE; TREPONEMA DENTICOLA; TYROSINE;

BACTERIA (MICROORGANISMS); TREPONEMA DENTICOLA;

EID: 27144462232     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051433n     Document Type: Article

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