A novel in vitro transcription method for producing siRNAs without specific sequence requirements

Molecular Biotechnology

Volumn 31, Issue 3, 2005, Pages 187-192

  Zhu, Xudong ^a   Li, Tao ^a   Dang, Ying ^a   Feng, Yi ^b   Huang, Peitang ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

RNA interference; RNase H; Small interfering RNA

Indexed keywords

RNA INTERFERENCE; RNASE H; SMALL INTERFERING RNA;

BIOSYNTHESIS; BIOTECHNOLOGY; DNA; GENES;

RNA;

RIBONUCLEASE; SMALL INTERFERING RNA;

ARTICLE; CONTROLLED STUDY; DNA SYNTHESIS; GENE EXPRESSION; GENE SEQUENCE; GENE SILENCING; GENE TARGETING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PROMOTER REGION; RESTRICTION MAPPING; RNA HYBRIDIZATION;

BASE SEQUENCE; GENE SILENCING; GENES, REPORTER; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; PROMOTER REGIONS (GENETICS); PROMOTER REGIONS, GENETIC; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 26844545323     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:31:3:187     Document Type: Article

References (14)

1. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.
2. Yang, D., Buchholz, F., Huang, Z., et al. (2002) Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 9942-9947.
3. Kawasaki, H., Suyama, E., Iyo, M., and Taira, K. (2003) siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells. Nucleic Acids Res. 31, 981-987.
4. Yang, D., Goga, A., and Bishop, J. M. (2004) RNA interference (RNAi) with RNase III-prepared siRNAs. Methods Mol. Biol. 252, 471-482.
5. Donze, O. and Picard, D. (2002) RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res. 30, e46.
6. Yu, J. Y., DeRuiter, S. L., and Turner, D. L. (2002) RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci USA 99, 6047-6052.
7. Milligan, J. F., Groebe, D. R., Witherell, G. W., and Uhlenbeck, O. C. (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15, 8783-8798.
8. Reynolds, A., Leake, D., Boese, Q., Scaringe, S., Marshall, W. S., and Khvorova, A. (2004) Rational siRNA design for RNA interference. Nat. Biotechnol. 22, 326-330.
9. Hohjoh, H. (2004) Enhancement of RNAi activity by improved siRNA duplexes. FEBS Lett. 557, 193-198.
10. Sohail, M., Doran, G., Riedemann, J., Macaulay, V., and Southern, E. M. (2003) A simple and cost-effective method for producing small interfering RNAs with high efficacy. Nucleic Acids Res. 31, e38.
11. Feldman, A. R. and Sen, D. (2001) A new and efficient DNA enzyme for the sequence-specific cleavage of RNA. J. Mol. Biol. 313, 283-294.
12. Bonaccio, M., Credali, A., and Peracchi, A. (2004) Kinetic and thermodynamic characterization of the RNA-cleaving 8-17 deoxyribozyme. Nucleic Acids Res. 32, 916-925.
13. Chi, J. T., Chang, H. Y., Wang, N. N., Chang, D. S., Dunphy, N., and Brown, P. O. (2003) Genomewide view of gene silencing by small interfering RNAs. Proc. Natl. Acad. Sci. USA 100, 6343-6346.
14. Hogrefe, H. H., Hogrefe, R. I., Walder, R. Y., and Walder, J. A. (1990) Kinetic analysis of Escherichia coli RNase H using DNA-RNA-DNA/DNA substrates. J. Biol. Chem. 265, 5561-5566.