Expression of bifunctional enzymes with xylose reductase and xylitol dehydrogenase activity in Saccharomyces cerevisiae alters product formation during xylose fermentation

Metabolic Engineering

Volumn 3, Issue 3, 2001, Pages 226-235

  Anderlund, Mikael ^a   Rådström, Peter ^a   Hahn Hägerdal, Bärbel ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC GENE; COMPLEX FORMATION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME SUBUNIT; FERMENTATION; FUNGAL GENE; GENE EXPRESSION; HYBRID PROTEIN; METABOLIC ENGINEERING; METABOLITE; XYLITOL DEHYDROGENASE; XYLOSE REDUCTASE;

PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE;

EID: 0034869823     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2001.0190     Document Type: Article

References (51)

1. Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation
2. Expression of genes in yeast using ADC1-promoter
3. Photometric methods for protein determination
4. The role of redox balances in the anaerobic fermentation of xylose by yeasts
5. NADH-linked aldose reductase: The key to anaerobic alcoholic fermentation of xylose by yeasts
6. Characterization of an artificial bifunctional enzyme, β-galactosidase/galactokinase, prepared by gene fusion
7. Physical and kinetic effects on introduction of various linker regions in β-galactosidase/galactose dehydrogenase fusion enzymes
8. Engineering of lactose metabolism in E. coli by introducing β-galactosidase/galactokinase fusion enzymes
9. Structure and mechanism of action of a multifunctional enzyme: Fatty acid synthase
10. Crucial role of the connecting region joining the two functional domains of yeast tryptophan synthetase
11. Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3, fusion protein
12. Development of yeast strains for the efficient utilisation of starch: Evaluation of constructs that express α-amylase and glucoamylase separately or as bifunctional fusion protein
13. A generalized theory of the transition time for sequential enzyme reactions
14. Evidence for electrostatic channelling in a fusion protein of malate dehydrogenase and citrate synthase
15. Xylitol production by recombinant Saccharomyces cerevisiae
16. The evolution of enzyme kinetic power
17. Strong control on the transit time in metabolic channelling
18. Effect of channelling on the concentration of bulk-phase intermediates as cytosolic proteins become more concentrated
19. Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene XYL2 and construction of a xylose-utilizing Saccharomyces cerevisiae transformant
20. Xylose fermentation by Saccharomyces cerevisiae
21. Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae
22. Cleavage of structural proteins during assembly of the head of bacteriophage T4
23. A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene
24. Metabolic studies on Saccharomyces cerevisiae containing fused citrate synthase/malate dehydrogenase
25. Characterization of a recombinant bifunctional enzyme, galactose dehydrogenase/bacterial luciferase, displaying an improved bioluminescence in a three-enzyme system
26. HPLC determination of xylulose formed by enzymatic xylose isomerization in lignocellulose hydrolysates
27. Construction of an artificial bifunctional enzyme, β-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling
28. Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae
29. A heterologous reductase affects the redox balance of recombinant Saccharomyces cerevisiae
30. The two monofunctional domains of octameric formiminotransferase-cyclodeaminase exist as dimers
31. Reaction products of yeast fermentations
32. Physiological significance of metabolic channeling
33. The efficiency of folding of some proteins is increased by controlled rates of translation in vivo
34. Metallothioneins with interdomain hinges expanded by insertion mutagenesis
35. Xylose fermentation by yeast. 4. Purification and kinetic studies of xylose reductase from Pichia stipitis
36. +-dependent xylitol dehydrogenase from yeast Pichia stipitis
37. Overexpression and structure-function analysis of a bioengineered IL-2/IL-6 chimeric lymphokine
38. High efficiency transformation of intact yeast cells by single stranded nucleic acids as carrier
39. Construction of an α-amylase/glucoamylase fusion gene and its expression in Saccharomyces cerevisiae
40. Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus
41. Complexes of sequential metabolic enzymes
42. Construction of xylose-assimilating Saccharomyces cerevisiae
43. Fed-batch fermentation of xylose by fast-growing mutant of xylose-assimilating recombinant Saccharomyces cerevisiae
44. Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase
45. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effect on product formation during xylose utilisation
46. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC 19 vectors
47. Nacleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: Indole-3-glycerol phosphate synthase