A ToxR-based two-hybrid system for the detection of periplasmic and cytoplasmic protein-protein interactions in Escherichia coli: Minimal requirements for specific DNA binding and transcriptional activation

Protein Engineering, Design and Selection

Volumn 18, Issue 10, 2005, Pages 477-486

  Hennecke, Frank ^a,b   Müller, Arne ^a,c   Meister, Roland ^a,d   Strelow, Astrid ^a,e   Behrens, Susanne ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b CYTOS BIOTECHNOLOGY AG   (Switzerland)

^c SANOFI   (France)

^d HANNOVER MEDICAL SCHOOL   (Germany)

^e diaDexus Inc   (United States)

Author keywords

DNA binding; Leucine zipper; Protein protein interaction; ToxR; Two hybrid system

Indexed keywords

CYTOLOGY; DIMERIZATION; DNA; ESCHERICHIA COLI;

DNA BINDING; LEUCINE ZIPPER; PROTEIN-PROTEIN INTERACTION; TOXR; TWO-HYBRID SYSTEM;

PROTEINS;

BACTERIAL PROTEIN; CYTOPLASM PROTEIN; DNA; PERIPLASMIC PROTEIN; TOXR PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; DIMERIZATION; DNA BINDING; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; CHOLERA TOXIN; CYTOPLASM; DNA, BACTERIAL; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; PERIPLASM; PROTEIN STRUCTURE, TERTIARY; SURFACE PLASMON RESONANCE; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

ESCHERICHIA COLI; PROKARYOTA; VIBRIO CHOLERAE;

EID: 26444598449     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzi053     Document Type: Article

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