Direct isolation of specific RNA-interacting proteins using a novel affinity medium

Nucleic Acids Research

Volumn 33, Issue 15, 2005, Pages 1-5

  Liu, Ding Gan ^a   Sun, Li ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANAZOLENE SODIUM; CCAAT ENHANCER BINDING PROTEIN BETA; GLASS; RNA; RNA BINDING PROTEIN; DNA;

3' UNTRANSLATED REGION; ARTICLE; BINDING AFFINITY; CELL CULTURE; COVALENT BOND; CULTURE MEDIUM; ELUTION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN RNA BINDING; RNA CONFORMATION; STAINING; CHEMISTRY; EVALUATION; ISOLATION AND PURIFICATION; METABOLISM; RNA PROBE; TUMOR CELL LINE;

3' UNTRANSLATED REGIONS; CCAAT-ENHANCER-BINDING PROTEIN-BETA; CELL LINE, TUMOR; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLASS; HUMANS; MASS SPECTROMETRY; RNA PROBES; RNA-BINDING PROTEINS;

EID: 26444560167     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni133     Document Type: Article

References (16)

1. Larson,D.E. and Sells,B.H. (1987) The function of proteins that interact with mRNA. Mol. Cell. Bioehem., 74, 5-15.
2. Stanton,G.J., Weigent,D.A., Fleischmann,W.R.,Jr, Dianzani,F. and Baron,S. (1987) Interferon review. Invest. Radiol., 22, 259-273.
3. Sagesser,R., Martinez,E., Tsagris,M. and Tabler,M. (1997) Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library. Nucleic Acids Res., 25, 3816-3822.
4. Lax,S.R., Lauer,S.J., Browning,K.S. and Ravel,J.M. (1986) Purification and properties of protein synthesis initiation and elongation factors from wheat germ. Methods Enzymol., 118, 109-128.
5. Graham,D.A., Deb,J.K. and Busch,H. (1984) Purification of p53/55 kinase from nuclear ribonucleoproteins of Namalwa cells. Mol. Cell. Biochem. 63, 39-52.
6. Dreyfuss,G. (1986) Structure and function of nuclear and cytoplasmic ribonucleoprotein particles. Annu. Rev. Cell. Biol., 2, 459-498.
7. Stark,B.C. (1995) (1995-1996) Early studies of native ribonuclease P, including discovery of its essential RNA component. Mol. Biol. Rep. 22, 95-97.
8. Jurica,M.S. and Moore,M.J. (2003) Pre-mRNA splicing: Awash in a sea of proteins. Mol. Cell., 12, 5-14.
9. Hook,B., Bernstein,D., Zhang,B. and Wickens,M. (2005) RNA-protein interactions in the yeast three-hybrid system: Affinity, sensitivity, and enhanced library screening. RNA, 11, 227-233.
10. Wysocki,V.H., Resing,K.A., Zhang,Q. and Cheng,G. (2005) Mass spectrometry of peptides and proteins. Methods, 35, 211-222.
11. Dong,R.C., Zhou,R.H., Lu,F.D. and Tao,W.Z. (1980) Establishment of SMMC7721, a human hepatocarcinoma cell line, and observations on its biological characteristics. Journal of 2nd Military Medical University (Shanghai), 1, 5-9(in Chinese).
12. Bronfman,M., Loyola,G. and Koenig,C.S. (1998) Isolation of intact organelles by differential centrifugation of digitonin-treated hepatocytes using a table Eppendorf centrifuge. Anal. Biochem., 255, 252-256.
13. Liu,D., Li,Z., Akira,S. and Kishimoto,T. (1996) Overexpression of a reversion-related protein in the revertant RR cells. Sci China C Life Sci., 39, 300-309(The original paper in Chinese published in Sci. China (ser.B), 25, 372-378, 1995).
14. Tolstonog,G.V., Sabasch,M. and Traub,P. (2002) Cytoplasmic intermediate filaments are stably associated with nuclear matrices and potentially modulate their DNA-binding function. DNA Cell Biol., 21, 213-239.
15. Liu,D.G., Jiang,Q.H., Wei,Y.Y., Sun,L., Fu,B.B., Zhao,F.K. and Zhou,Q. (2003) Gene exprssion profile favoring phenotypic reversion: A clue for mechanism of tumor suppression by NF-IL6 3′UTR. Cell Res., 13 509-514.
16. Helfand,B.T., Chang,L. and Goldman,R.D. (2004) Intermediate filaments are dynamic and motile elements of cellular architecture. J Cell Sci. 117, 133-141.