Isolation of intact organelles by differential centrifugation of digitonin-treated hepatocytes using a table Eppendorf centrifuge

Analytical Biochemistry

Volumn 255, Issue 2, 1998, Pages 252-256

  Bronfman, Miguel ^a   Loyola, Gloria ^a   Koenig, Cecilia S ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

CENTRIFUGES; ENZYMES; MITOCHONDRIA;

CYTOSOLIC FRACTIONS; DIFFERENTIAL CENTRIFUGATION; MITOCHONDRIAL FRACTION; PARTICULATE FRACTION; PHOSPHORYLATED PROTEINS; SUBCELLULAR FRACTIONATION; SUBCELLULAR LOCALIZATIONS; SUBCELLULAR ORGANELLES;

CENTRIFUGATION;

DIGITONIN; PHOSPHOPROTEIN;

ARTICLE; CELL FRACTIONATION; CELL ORGANELLE; CELLULAR DISTRIBUTION; CENTRIFUGATION; LIVER CELL; PRIORITY JOURNAL; PROTEIN DETERMINATION;

EID: 0032518312     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2453     Document Type: Article

References (8)

1. de Duve, C., Pressman, B. C., Giannetto, R., Wattiaux, R., and Applemans, F. (1955) Biochem. J. 60, 604-617.
2. Bronfman, M., Inestrosa, N. C., Nervi, F., and Leighton, F. (1984) Biochem. J. 224, 709-720.
3. Bronfman, M., and Leighton, F. (1984) Biochem. J. 224, 721-730.
4. Berry, M. N., and Friend, D. S. (1969) J. Cell. Biol. 43, 596-620.
5. Burton, K. (1968). in Methods in Enzymology, Vol. XII, Nucleic Acids, Part B (Grossman, L., and Moldave, K., Eds.), pp. 163-166, Academic Press, New York.
6. Leighton, F., Coloma, L., and Koenig, C. (1975) J. Cell. Biol. 67, 281-309.
7. Zuurendank, P. F., and Tager, J. M. (1974) Biochim. Biophys. Acta 333, 383-399.
8. Janski, A. M., and Cornell, N. W. (1980) Biochem. J. 186, 423-429.