Lipid A components from Pseudomonas aeruginosa PAO1 (serotype O5) and mutant strains investigated by electrospray ionization ion-trap mass spectrometry

Journal of Mass Spectrometry

Volumn 39, Issue 5, 2004, Pages 505-513

  Bedoux, Gilles ^a   Vallée Réhel, Karine ^a   Kooistra, Oliver ^b   Zähringer, Ulrich ^b   Haras, Dominique ^a  

^a UNIVERSITÉ DE BRETAGNE SUD   (France)

^b RESEARCH CENTER BORSTEL   (Germany)

Author keywords

Electrospray ionization mass spectrometry; Ion trap analyzer; Lipid A; Lipopolysaccharide; Pseudomonas aeruginosa

Indexed keywords

ACETIC ACID; HYDROLYSIS; IONIZATION; MASS SPECTROMETRY;

ELECTROSPRAY IONIZATION (ESI); NEGATIVE ION MODES;

LIPIDS;

ACETIC ACID; BACTERIUM LIPOPOLYSACCHARIDE; DISACCHARIDE; FATTY ACID; GLUCOSAMINE; LIPID A;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; BACTERIUM MUTANT; CHEMICAL STRUCTURE; CONTROLLED STUDY; DERIVATIZATION; ELECTROSPRAY MASS SPECTROMETRY; FRAGMENTATION REACTION; HYDROLYSIS; ION TRAP MASS SPECTROMETRY; NEGATIVE ION ELECTROSPRAY; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; PURIFICATION; SENSITIVITY ANALYSIS; SEROTYPE; STRAIN DIFFERENCE; WILD TYPE;

LIPID A; MOLECULAR STRUCTURE; MUTATION; PSEUDOMONAS AERUGINOSA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 2642529606     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.611     Document Type: Article

References (46)

1. Govan JRW, Deretic V. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 1996; 60: 539.
2. Cryz SJ Jr, Pitt TL, Fürer E, Germanier R. Role of lipopolysaccharide in virulence of Pseudomonas aeruginosa. Infect. Immun. 1984; 44: 508.
3. Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldberg JB, Prince A. Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infect. Immun. 1996; 64: 37.
4. Costerton JW. Cystic fibrosis pathogenesis and the role of biofilms in persistent infection. Trends Microbiol. 2001; 9: 50.
5. Hancock REW, Mutharia LM, Chan L, Darveau RP, Speert DP, Pier GB. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum sensitive, nontypable strains deficient in lipopolysaccharide side chains. Infect. Immun. 1983; 42: 170.
6. Arsenault TL, Hugues DW, MacLean DB, Szarek WA, Kropinski AMB, Lam JS. Structural studies on the polysaccharide portion of 'A-Band' lipopolysaccharide from a mutant (AK1401) of Pseudomonas aeruginosa strain PAO1. Can. J. Chem. 1991; 69: 1273.
7. Lüderitz O, Freudenberg MA, Galanos C, Lehmann V, Rietschel ET, Shaw DH. Lipopolysaccharides of Gram-negative bacteria. In Membrane Lipids of Procaryotes, Current Topics in Membranes and Transport, Vol. 17, Razin S, Rottem S (eds). Academic Press: New York, 1982; 79.
8. Heinrichs DE, Yethon JA, Whitfield C. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol. Microbiol. 1998; 30: 221.
9. Knirel YA. Polysaccharide antigens of Pseudomonas aeruginosa. Crit. Rev. Microbiol. 1990; 17: 273.
10. Knirel YA, Paramonov NA, Vinogradov EV, Shashkov AS, Dmitriev BA, Kochetkov NK, Kholodkova EV, Stanislavsky ES. Somatic antigens of Pseudomonas aeruginosa, the structure of O-specific polysaccharide of P. aeruginosa O3 (Lanyi), O25 (Wokatsch) and Fisher immunotypes 3 and 7. Eur. J. Biochem. 1987; 167: 549.
11. Holst O. Chemical structure of the core region of lipopolysaccharides. In Endotoxin in Health and Disease, Brade H, Opal SM, Vogel SN, Morrison DC (eds). Marcel Dekker: New York, 1999; 115.
12. Rietschel ET, Kirikae T, Schade UF, Mamat U, Schmidt G, Loppnow H, Ulmer AJ, Zähringer U, Seydel U, Di Padova F, Schreier M, Brade H. Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB J. 1994; 8: 217.
13. Darveau RP. Lipid A diversity and the innate host response to bacterial infection. Curr. Opin. Microbiol. 1998; 1: 36.
14. Persing DH, Coler RN, Lacy MJ, Johnson DA, Baldridge JR, Hershberg RM, Reed SG. Taking toll: lipid A mimetics as adjuvants and immunomodulators. Trends Microbiol. 2002; 10: S32.
15. Rietschel ET, Brade L, Holst O, Kulshin VA, Lindner B, Moran AP, Schade UF, Zähringer U, Brade H. Molecular structure of bacterial endotoxin in relation to bioactivity. In Cellular and Molecular Aspects of Endotoxin Reactions, Nowotny A, Spitzer JJ, Ziegler EJ (eds). Elsevier Science: Amsterdam: 1990; 15.
16. Zähringer U, Lindner B, Rietschel ET. Molecular structure of lipid A, the endotoxic center of bacterial lipopolysaccharides. Adv. Carbohydr. Chem. Biochem. 1994; 50: 211.
17. Bhat R, Marx A, Galanos C, Conrad RS. Structural studies of lipid A from Pseudomonas aeruginosa PAO1: occurrence of 4-amino-4-deoxyarabinose. J. Bacteriol. 1990; 172: 6631.
18. Holst O, Müller-Loennies S, Lindner B, Brade H. Chemical structure of the lipid A of Escherichia coli J-5. Eur. J. Biochem. 1993; 214: 695.
19. Karunaratne DN, Richards JC, Hancock REW. Characterization of lipid A from Pseudomonas aeruginosa O-antigenic B band lipopolysaccharide by 1D and 2D NMR and mass spectral analysis. Arch. Biochem. Biophys. 1992; 299: 368.
20. Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET. Structural characterization of the lipid A component of Pseudomonas aeruginosa wild type and rough mutant lipopolysaccharides. Eur. J. Biochem. 1991; 198: 697.
21. Ernst RK, Yi EC, Guo L, Lim KB, Burns JL, Hackett M, Miller SI. Specific lipopolysaccharide found in cystic fibrosis Airway Pseudomonas aeruginosa. Science 1999; 286: 1561.
22. Karibian D, Deprun C, Caroff M. Use of plasma desorption mass spectrometry in structural analysis of endotoxins: effects on lipidA of different acid treatments. In Bacterial Endotoxins: Lipopolysaccharides from Genes to Therapy, Levin J, Alving CR, Munford RS, Redl H (eds). Wiley-Liss: New York, 1995; 103.
23. Corsaro MM, Dal Piaz F, Lanzetta R, Parilli M. Lipid A structure of Pseudoalteromonas haloplanktis TAC 125: use of electrospray ionization tandem mass spectrometry for the determination of fatty acid distribution. J. Mass Spectrom. 2002; 37: 481.
24. Kussak A, Weintraub A. Quadrupole ion-trap mass spectrometry to locate fatty acids on lipidA from Gram-negative bacteria. Anal. Biochem. 2002; 307: 131.
25. Harrata AK, Domelsmith LN, Cole RB. Electrospray mass spectrometry for characterization of lipid A from Enterobacter agglomerans. Biol. Mass Spectrom. 1993; 22: 59.
26. Chan S, Reinhold VN. Detailed structural characterization of lipid A: electrospray ionization coupled with tandem mass spectrometry. Anal. Biochem. 1994; 218: 63.
27. Zähringer U, Lindner B, Rietschel ET. Chemical structure of lipidA: recent advances in structural analysis of biologically active molecules. In Endotoxin in Health and Disease, Brade H, Opal SM, Vogel SN, Morrison DC (eds). Marcel Dekker: New York, 1999; 93.
28. Zielinski NA, Chakrabarty AM, Berry A. Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase. J. Biol. Chem. 1991; 266: 9754.
29. Brint JM, Ohman DE. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of rhlR-rhlI, another set of regulators in strains PAO1 with homology to the autoinducer-responsive luxR-luxI family. J. Bacteriol. 1995; 177: 7155.
30. Westphal O, Jann K. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 1965; 5: 83.
31. Inzana TJ. Electrophoretic heterogeneity and interstrain variation of the lipopolysaccharide of Haemophilus influenzae. J. Infect. Dis. 1983; 148: 492.
32. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680.
33. Tsai CM, Frash CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 1982; 119: 115.
34. Brade H, Galanos C, Lüderitz O. Differential determination of the 3-deoxy-D-manno-octulosonic acid residues in lipopolysaccharides of Salmonella minnesota rough mutants. Eur. J. Biochem. 1983; 131: 195.
35. Strominger JL, Park JT, Thompson RE. Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. J. Biol. Chem. 1959; 234: 3263.
36. Lowry OH, Roberts NR, Leiner KY, Wu ML, Farr AL. The quantitative histochemistry of brain. J. Biol. Chem. 1954; 207: 1.
37. Sawardeker JS, Sloneker JH, Jeanes A. Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Anal. Chem. 1965; 37: 1602.
38. Sadovskaya I, Brisson JR, Lam JS, Richards JC, Altman E. Structural elucidation of the lipopolysaccharide core regions of the wild-type strain PAO1 and O-chain-deficient mutant strains AK1401 and AK 1012 from Pseudomonas aeruginosa serotype O5. Eur. J. Biochem. 1998; 255: 673.
39. Sadovskaya I, Brisson JR, Thibault P, Richards JC, Lam JS, Altman E. Structural characterization of the outer core and the O-chain linkage region of lipopolysaccharide from Pseudomonas aeruginosa serotype O5. Eur. J. Biochem. 2000; 267: 1640.
40. De Kievit TR, Gillis R, Marx S, Brown C, Iglewski BH. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Appl. Environ. Microbiol. 2001; 67: 1865.
41. Yang H, Matewish M, Loubens I, Storey DG, Lam JS, Jin S. migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-mass lipopolysaccharide. Microbiology 2000; 146: 2509.
42. Coyne MJ, Russell KS, Coyle CL, Goldberg JB. The Pseudomonas aeruginosa algC gene encodes phosphoglucomutase, required for the synthesis of a complete lipopolysaccharide core. J. Bacteriol. 1994; 176: 3500.
43. Goldberg JB, Hatano K, Pier GB. Synthesis of lipopolysaccharide O side chains by Pseudomonas aeruginosa PAO1 requires the enzyme phosphomannomutase. J. Bacteriol. 1993; 175: 1605.
44. Yi EC, Hackett M. Rapid isolation method for lipopolysaccharide and lipid A from Gram-negative bacteria. The Analyst. 2000; 125: 651.
45. Costello CE, Vath JE. Tandem mass spectrometry of glycolipids. Methods Enzymol. 1990; 193: 738.
46. Kooistra O, Bedoux G, Brecker L, Lindner B, Sánchez Carballo P, Haras D, Zähringer U. Structure of a highly phosphorylated lipopolysaccharide core in the ΔalgC mutants derived from Pseudomonas aeruginosa wild-type strains PAO1 (serogroup O5 and PAC1R (serogroup O3). Carbohydr. Res. 2003; 338: 2667.