A proteomic method for the analysis of changes in protein concentrations in response to systemic perturbations using metabolic incorporation of stable isotopes and mass spectrometry

Proteomics

Volumn 5, Issue 14, 2005, Pages 3563-3570

  Gustavsson, Niklas ^a,b   Greber, Boris ^a   Kreitler, Thomas ^a   Himmelbauer, Heinz ^a   Lehrach, Hans ^a   Gobom, Johan ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^b Department of Plant Biochemistry ^*  (Sweden)

Author keywords

MS; Protein turnover; PTM; Quantitative proteomics; Stable isotope labeling

Indexed keywords

HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 70; VIMENTIN;

ARTICLE; CELL CULTURE; CULTURE MEDIUM; HALF LIFE TIME; HEAT STRESS; HELA CELL; HUMAN; HUMAN CELL; IMAGE ANALYSIS; INFORMATION PROCESSING; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; PROTEOMICS; STEADY STATE;

HALF-LIFE; HEAT; HEAT-SHOCK PROTEINS; HELA CELLS; HUMANS; HYDROLYSIS; ISOTOPE LABELING; NITROGEN ISOTOPES; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 25844518423     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200401193     Document Type: Article

References (16)

1. Moritz, B., Meyer, H. E., Proteomics 2003, 3, 2208-2220.
2. Ong, S. E., Foster, L. J., Mann, M., Methods 2003, 29, 124-130.
3. Oda, Y., Huang, K., Cross, F. R., Cowburn, D., Chait, B. T., Proc. Natl. Acad. Sci. USA 1999, 96, 6591-6596.
4. Pratt, J. M., Petty, J., Riba-Garcia, I., Robertson, D. H. L. et al., Mol. Cell. Proteomics 2002, 1, 579-591.
5. Cargile, B. J., Bundy, J. L., Grunden, A. M., Stephenson, J. L., Anal. Chem. 2004, 76, 86-97.
6. Lindquist, S., Craig, E. A., Annu. Rev. Genet. 1988, 22, 631-677.
7. Laemmli, U. K., Nature 1970, 227, 680-685.
8. Neuhoff, V., Arold, N., Taube, D., Ehrhardt, W., Electrophoresis 1988, 9, 255-262.
9. Gobom, J., Schuerenberg, M., Mueller, M., Theiss, D. et al., Anal. Chem. 2001, 73, 434-438.
10. Gobom, J., Mueller, M., Egelhofer, V., Theiss, D. et al., Anal. Chem. 2002, 74, 3915-3923.
11. Perkins, D. N., Pappin, D. J. C., Creasy, D. M., Cottrell, J. S., Electrophoresis 1999, 20, 3551-3567.
12. Parsell, D. A., Lindquist, S., Annu. Rev. Genet. 1993, 27, 437-496.
13. Fuchs, E., Weber, K., Annu. Rev. Biochem. 1994, 63, 345-382.
14. Gerner, C., Vejda, S., Gelbmann, D., Bayer, E. et al., Mol. Cell. Proteomics 2002, 1, 528-537.
15. Jensen, O. N., Curr. Opin. Chem. Biol. 2004, 8, 33-41.
16. Nakanishi, K., Maruyama, M., Shibata, T., Morishima, N., J. Biol. Chem. 2001, 276, 41237-41244.