Electron microscopy of cultured angiogenic endothelial cells

Microscopy Research and Technique

Volumn 67, Issue 5, 2005, Pages 248-259

  Hirschberg, Ruth M ^a   Sachtleben, Monika ^a   Plendl, Johanna ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Apoptosis; Bovine; Improved processing method; In vitro angiogenesis; Morphology; Scanning electron microscopy; Ultrastructure

Indexed keywords

CELL DEATH; ELECTRONS; ENDOTHELIAL CELLS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MAMMALS;

ANGIOGENESIS; BOVINE; CELLULARS; ENDOTHELIAL-CELLS; IMPROVED PROCESSING METHOD; IN VITRO ANGIOGENESIS; IN-VITRO; PROCESSING METHOD; SPATIAL ARRANGEMENTS; ULTRASTRUCTURE;

SCANNING ELECTRON MICROSCOPY;

ANGIOGENESIS; ANIMAL CELL; APOPTOSIS; ARTICLE; BIOCHEMISTRY; CELL CULTURE; CELL DIFFERENTIATION; CELL JUNCTION; CELL MIGRATION; CELL PROLIFERATION; CELL SPECIFICITY; CORPUS LUTEUM; COW; DIAGNOSTIC VALUE; ELECTRON MICROSCOPY; ENDOTHELIUM CELL; IN VITRO STUDY; INTUSSUSCEPTION; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; THREE DIMENSIONAL IMAGING; TRANSMISSION ELECTRON MICROSCOPY;

BOVINAE;

EID: 25844492946     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jemt.20204     Document Type: Article

References (61)

1. Akita M, Murata E, Merker HJ, Kaneko K. 1997. Formation of new capillary-like tubes in a three-dimensional in vitro model. Ann Anat 179:137-148.
2. Aird WC. 2003. Endothelial cell heterogeneity. Crit Care Med 31:8221-8230.
3. Auerbach R, Lewis R, Shinners B, Kubai L, Akhtar N. 2003. Angiogenesis assays: a critical overview. Clin Chem 49:32-40.
4. Augustin HG. 1998. Antiangiogenic tumour therapy: will it work? Trends Pharmacol Sci 19:216-222.
5. Augustin HG. 2000. Vascular morphogenesis in the ovary. Baillieres Best Pract Res Clin Obstet Gynaecol 14:867-882.
6. Augustin HG. 2001. Tubes, branches, and pillars. The many ways of forming a new vasculature. Circ Res 89:645-647.
7. Bahramsoltani M. 2004. Quantifizierung der Angiogenese und Antiangiogenese in vitro. (Quantitation of angiogenesis and antiangiogenesis in vitro.) Dissertation thesis. Faculty of Veterinary Anatomy, Freie Universität Berlin.
8. Bahramsoltani M, Plendl J. 2004. Ein neues in vitro-Modell zur Quantifizierung der Angiogenese in vitro. (A new in vitro model to quantify angiogenesis). ALTEX 21:227-244.
9. Bazzoni G, Dejana E. 2004. Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis. Physiol Rev 84:869-901.
10. Bhushan M, Young HS, Brenchley PEC, Griffiths CEM. 2002. Recent advances in cutaneous angiogenesis. Brit J Dermatol 147:418-425.
11. Braet F, De Zanger R, Wisse E. 1997. Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells. J Microsc 186:84-87.
12. Burri PH, Djonov V. 2002. Intussusceptive angiogenesis-the alternative to capillary sprouting. Mol Asp Med 23:S1-S27.
13. Carmeliet P. 2002. The development of blood vessels: cellular and molecular mechanisms. In: Tomanek RJ, editor. Assembly of the vasculature and its regulation. Boston: Birkhäuser. Chapter 1, pp. 1-20.
14. Davis GE, Bayless KJ, Mavila A. 2002. Molecular basis of endothelial cell morphogenesis in three-dimensional extracellular matrices. Anat Rec 268:252-275.
15. Davis JS, Rueda BR, Spanel-Borowski K. 2003. Microvascular endothelial cells of the corpus luteum. Reprod Biol Endocrinol 1:89-104.
16. Dvorak AM, Wiberg L, Monahan-Earley RA, Galli SJ. 1990. A simple technique to facilitate the ultrastructural analysis of cells in soft agar culture systems: demonstration of the development in vitro of morphologically mature mast cells and phagocytic macrophages from the bone marrow cells of genetically mast cell-deficient W/Wv or congenic normal mice. Lab Invest 62:774-781.
17. Ferrara N. 2001. Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am J Physiol Cell Physiol 280: C1358-C1366.
18. Folkman J. 1995. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med 1:27-32.
19. Folkman J. 1996. Fighting cancer by attacking its blood supply. Sci Am 275:150-154.
20. Folkman J, Haudenschild C. 1980. Angiogenesis by capillary endothelial cells in culture. Trans Ophthalmol Soc UK 100:346-353.
21. Haller C, Kübler W. 1999. Zellpolarität im Herz-Kreislauf- System. (Cell polarity in the cardiovascular system.) Z Kardiol 88:324-330.
22. Hirschberg RM, Plendl J. 2005. Pododermal angiogenesis and angio-adaptation in the bovine claw. Microsc Res Tech 66:145-155.
23. Hirschberg RM, Bahramsoltani M, Hünigen H, Plendl J. 2003. Heterogeneity of angiogenic endothelial cells in vitro. J Vasc Res 40:199.
24. Hughes S, Chan-Ling T. 2000. Roles of endothelial cell migration and apoptosis in vascular remodeling during development of the central nervous system. Microcirculation 7:317-333.
25. Käßmeyer S, Plendl J. 2004. Endothelial progenitor cells trigger vascular development: an in vitro study. J Vase Res 41:476.
26. Kaufman DS, Lewis RL, Hanson ET, Auerbach R, Plendl J, Thomson JA. 2004. Functional endothelial cells derived from rhesus monkey embryonic stem cells. Blood 103:1325-1332.
27. Lienau J. 2003. Etablierung von in vitro-Modellen der Angiogenese muriner und humaner Endothelzellen sowie deren Transfektion mit verschiedenen Plasmidkonstrukten (An in vitro model of angiogenesis of endothelial cells from the murine myocardium and from the human neonatal foreskin and transfection of endothelial cells with different plasmid constructs). Dissertation thesis, Faculty of Veterinary Medicine, Freie Universität, Berlin.
28. Lienau J, Kaletta C, Teifel M, Plendl J, Hirschberg RM. 2003. Morphological characteristics of transfected human angiogenic endothelial cells. Ann Anat Suppl 185:93-94.
29. Lienau J, Kaletta C, Teifel M, Naujoks K, Bhoola K, Plendl J. 2005. Angiogenesis of human microvascular endothelial cells: morphology and transfection: an in vitro three-dimensional model. Biol Chem 386:227-244.
30. Liepins A, Bustamante O. 1994. Cell injury and apoptosis. Scan Microsc 8:631-643.
31. Majumdar SK, Valdellon JA, Brown KA. 2001. In vitro investigations on the toxicity and cell death induced by tamosifen on two non-breast cancer cell types. J Biomed Biotechnol 1:99-107.
32. Malorni W, Fais S, Fiorentini C. 1997. Morphological aspects of apoptosis. In: Cossarizza A, Boraschi D, editors. Apoptosis: a laboratory manual of experimental methods. West Lafayette, USA: Purdue University Cytometry Laboratories. The Purdue Cytometry CD-ROM Volume 4.
33. Mansy SS. 2004. Agarose cell block: innovated technique for the processing of urine cytology for electron microscopy examination. Ultrastruct Pathol 28:15-21.
34. Masuda H, Kawamuara K, Nanjo H, Sho E, Komatsu M, Sugiyama T, Sugita A, Asari Y, Kobayashi M, Ebina T, Hoshi N, Singh TM, Xu C, Zarins CK. 2003. Ultrastructure of endothelial cells under flow alteration. Microsc Res Tech 60:2-12.
35. Meyer GT, Matthias LJ, Noack L, Vadas MA, Gamble JR. 1997. Lumen formation during angiogenesis in vitro involves phagocytic activity, formation and secretion of vacuoles, cell death and capillary tube remodelling by different populations of endothelial cells. Anat Rec 249:327-340.
36. Michel J-P. 2003. Anoïkis in the cardiovascular system. Arterioscler Thromb Vasc Biol 23:2146-2154.
37. Middleton J, Patterson AM, Gardner L, Schmutz C, Ashton BA. 2002. Leukocyte extravasation: chemokine transport and presentation by the endothelium. Blood 100:3853-3860.
38. Moskaluk CA, Stoler MH. 2002. Agarose mold embedding of cultured cells for tissue microarrays. Diagn Mol Pathol 11:234-238.
39. Patan S. 2000. Vasculogenesis and angiogenesis as mechanisms of vascular network formation, growth and remodelling. J Neuroonc 50:1-15.
40. Patan S. 2004. Vasculogenesis and angiogenesis. Cancer Treat Res 117:3-32.
41. Pepper MS, Mandriota SJ, Jeltsch M, Kumar V, Alitalo K. 1998. VEGF-C synergizes with bFGF and VEGF in the induction of angiogenesis in vitro and alters endothelial cell extracellular proteolytic activity. J Cell Physiol 177:439-452.
42. Plendl J. 2000. Angiogenesis in the bovine ovary. A Review. Anat Histol Embryol 29:257-266.
43. Plendl J, Neumüller C, Sinowatz F. 1996. Differences between endothelial cells isolated from the corpus luteum of the estrous cycle and the corpus luteum of pregnancy. Biol Cell 87:179-188.
44. Plendl J, Snyman C, Naidoo S, Sawant S, Mahaber R, Bhoola K. 2000. Expression of tissue kallikrein and kinin receptors in angiogenic microvascular endothelial cells. Biol Chem 381:1103-1115.
45. Plendl J, Gilligan BJ, Wang S-J, Lewis R, Shiners B, Vandenbroeck K, Auerbach R. 2002a. Primitive endothelial cell lines from the porcine embryonic yolk sac. In Vitro Cell Dev Biol Anim 38:334-342.
46. Plendl J, Hirschberg RM, Hünigen H. 2002b. Mechanismen der vaskulären Entwicklung und Regression. Eine aktuelle Übersicht (Mechanisms of vascular development and regression. An overview). Tierärztl Prax 30:243-253.
47. Polverini PJ. 1995. The pathophysiology of angiogenesis. Crit Rev Oral Biol Med. 6:230-247.
48. Reville WJ, Heapes MM, O'Sullivan VR. 1994. A survey to assess the ultrastructural preservation of fixed biological samples after air-drying from tetramethylsilane. J Electron Microsc 43:111-115.
49. Risau W. 1997. Mechanisms of angiogenesis. Nature 386:671-674.
50. Sho E, Sho M, Singh TM, Xu C, Zarins CK, Masuda H. 2001. Blood flow decrease induces apoptosis of endothelial cells in previously dilated arteries resulting from chronic high blood flow. Arterioscler Thromb Vasc Biol 21:1139-1145.
51. Slizova D, Krs O, Pospisilova B. 2003. Alternative method of rapid drying vascular specimens for scanning electron microscopy. J Endovasc Ther 10:285-287.
52. Spanel-Borowski K, Fenyves A. 1994. The heteromorphology of cultured microvascular endothelial cells. Arzneim-Forsch/Drug Res 44:385-391.
53. Stevenson DP, Collins WP, Farzaneh F, Hata K, Miyazaki K. 1998. Thymidine phosphorylase activity and prodrug effects in a three-dimensional model of angiogenesis. Am J Pathol 153:1573-1578.
54. Taniguchi Y, Tamatani R, Kawarai Y. 1994. A reliable method of embedding a small amount of dispersed cells for electron microscopy. J Electron Microsc 43:48-50.
55. Thommen R, Humar R, Misevic G, Pepper MS, Han AWA, John M, Battegay EJ. 1997. PDGF-BB increases endothelial migration and cord movements during angiogenesis in vitro. J Cell Biochem 64:403-413.
56. Tolsma SS, Stack SM, Bouck N. 1997. Lumen formation and other angiogenic activities of cultured capillary endothelial cells are inhibited by Thrombospondin-1. Microvasc Res 54:13-26.
57. Vailhè B, Vittet D, Feige J-J. 2001. In vitro models of vasculogenesis and angiogenesis. Lab Invest 81:439-452.
58. Wang JH, Xing QT, Yuan MB. 2004. Antineoplastic effects of octreotie on human gallbladder cancer cells in vitro. World J Gastroenterol 10:1043-1046.
59. Widehn S, Kindblom LG. 1990. Agarose embedding: a new method for the ultrastructural examination of the in-situ morphology of cell cultures. Ultrastruct Pathol 14:81-85.
60. Wilting J, Christ B. 1996. Embryonic angiogenesis-a review. Naturwissenschaften 83:153-164.
61. Yuan LC, Gulyas BJ. 1981. An improved method for processing single cells for electron microscopy utilizing agarose. Anat Rec 201:273-281.