Radial and axial variations of bacteria within the cecum and proximal colon of guinea pigs revealed by PCR-DGGE

Bioscience, Biotechnology and Biochemistry

Volumn 69, Issue 9, 2005, Pages 1790-1792

  Takahashi, Toru ^a   Karita, Shuichi ^a   Yahaya, M Sani ^a   Goto, Masakazu ^a  

^a MIE UNIVERSITY   (Japan)

Author keywords

Denaturing gradient gel electrophoresis (DGGE); Digesta; Intestinal lumen; Large intestine; Microbiota

Indexed keywords

BACTERIA; DIGESTIVE SYSTEM; ELECTROPHORESIS; GELS; IMMUNOLOGY;

AXIAL VARIATION; DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE); DIGESTA; INTESTINAL LUMEN; LARGE INTESTINAL; MICROBIOTA;

BIODIVERSITY;

ANIMAL; ARTICLE; BACTERIUM; CECUM; CLASSIFICATION; COLON; ELECTROPHORESIS; GENETICS; GUINEA PIG; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION;

ANIMALS; BACTERIA; CECUM; COLON; ELECTROPHORESIS; GUINEA PIGS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CAVIA; CAVIA PORCELLUS;

EID: 25844471512     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.69.1790     Document Type: Article

References (14)

1. Takahashi, T., and Sakata, T., Large particles increase viscosity and yield stress of pig cecal contents without changing basic viscoelastic properties. J. Nutr., 132, 1026-1030 (2002).
2. Takahashi, T., Yamanaka, N., Sakata, T., and Ogawa, N., Influences of solid particles on the viscous properties of intestinal contents and intestinal tissue weight in rats. Nippon Eiyo Shokuryo Gakkaishi, 56, 199-205 (2003).
3. Takahashi, T., and Sakata, T., Viscous properties of pig cecal contents and the contribution of solid particles to viscosity. Nutrition, 20, 377-382 (2004).
4. Takahashi, T., Goto, M., and Sakata, T., Viscoelastic properties of the small intestinal and caecal contents of the chicken. Br. J. Nutr., 91, 867-872 (2004).
5. Takahashi, T., and Sakata, T., Insoluble dietary fibers: the major modulator for the viscosity and flow behavior of digesta. Foods Food Ingredients J. Jpn., in press.
6. Watanabe, T., Yajima, T., and Sakata, T., Regional heterogeneity of bacteria and their glycosidase activities across and along the colonic segments of rats. Lett. Appl. Microbiol., 8, 29-31 (1989).
7. Takahashi, T., and Sakaguchi, E., Role of the furrow of the proximal colon in the production of soft and hard feces in nutrias, Myocastor coypus. J. Comp. Physiol. B, 170, 531-535 (2000).
8. Japanese Association for Laboratory Animal Science, The guideline for animal experimentation. Exp. Anim., 36, 285-288 (1987).
9. Takahashi, T., and Sakaguchi, E., Behaviors and nutritional importance of coprophagy in captive adult and young nutrias (Myocastor coypus). J. Comp. Physiol. B, 168, 281-288 (1998).
10. Pedro, M. S., Murata, S., Hazaka, M., Shimada, R., Yoshida, C., Hiura, K., Ishii, M., and Igarashi, Y., Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter. J. Biosci. Bioeng., 91, 159-165 (2001).
11. Sakata, T., and von Engelhardt, W., Luminal mucin in the large intestine of mice, rats and guinea pigs. Cell Tissue Res., 219, 629-635 (1981).
12. Gong, J., Forster, R. J., Yu, H., Chambers, J. R., Sabour, P. M., Wheatcroft, R., and Chen, S., Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen. FEMS Microbiol. Lett., 208, 1-7 (2002).
13. Schmitt-Wagner, D., Friedrich, M. W., Wagner, B., and Brune, A., Phylogenetic diversity, abundance, and axial distribution of bacteria in the intestinal tract of two soil-feeding termites (Cubitermes spp.). Appl. Environ. Microbiol., 69, 6007-6017 (2003).
14. Sindhu, S. C., and Khetarpaul, N., Effect of probiotic fermentation on antinutrients and in vitro protein and starch digestibilities of indigenously developed RWGT food mixture. Nutr. Health, 16, 173-181 (2002).