Inhibition of Sp1 activity by a decoy PNA-DNA chimera prevents urokinase receptor expression and migration of breast cancer cells

Biochemical Pharmacology

Volumn 70, Issue 9, 2005, Pages 1277-1287

  Zannetti, Antonella ^a   Del Vecchio, Silvana ^a   Romanelli, Alessandra ^a   Scala, Stefania ^a   Saviano, Michele ^a   Cali', Gaetano ^b   Stoppelli, M Patrizia ^c   Pedone, Carlo ^a   Salvatore, Marco ^d  

^a CNR   (Italy)

^b CNR   (Italy)

^c INSTITUTE OF GENETICS AND BIOPHYSICS ADRIANO BUZZATI TRAVERSO   (Italy)

^d UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Breast cancer; Peptide nucleic acid; PNA DNA chimera; Sp1 transcription factor; Urokinase receptor

Indexed keywords

COMPLEMENTARY DNA; DNA; LIPOSOME; OLIGONUCLEOTIDE; PEPTIDE NUCLEIC ACID; TRANSCRIPTION FACTOR SP1; UROKINASE; UROKINASE RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; BREAST CANCER; CANCER CELL; CANCER INHIBITION; CELL MIGRATION; CELL MOTILITY; CHIMERA; COVALENT BOND; DNA STRAND; DOWN REGULATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INHIBITION KINETICS; METASTASIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; RECEPTOR UPREGULATION;

BREAST NEOPLASMS; CELL LINE, TUMOR; CELL MOVEMENT; DNA; FEMALE; HUMANS; PEPTIDE NUCLEIC ACIDS; RECEPTORS, CELL SURFACE; SP1 TRANSCRIPTION FACTOR;

EID: 25644442324     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bcp.2005.07.024     Document Type: Article

References (37)

1. A.F. Chambers, A.C. Groom, and I.C. MacDonald Dissemination and growth of cancer cells in metastatic sites Nat Rev Cancer 2 2002 563 572
2. P.A. Andreasen, R. Egelund, and H.H. Petersen The plasminogen activation system in tumor growth, invasion, and metastasis Cell Mol Life Sci 57 2000 25 40
3. F. Blasi, and P. Carmeliet uPAR: a versatile signalling orchestrator Nat Rev Mol Cell Biol 3 2002 932 943
4. L. Ossowski, and J.A. Aguirre-Ghiso Urokinase receptor and integrin partnership: coordination of signaling for cell adhesion, migration and growth Curr Opin Cell Biol 12 2000 613 620
5. N. Harbeck, R.E. Kates, M.P. Look, M.E. Meijer-Van Gelder, J.G. Klijn, and A. Kruger Enhanced benefit from adjuvant chemotherapy in breast cancer patients classified high-risk according to urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (n = 3424) Cancer Res 62 2002 4617 4622
6. M.P. Look, W.L. van Putten, M.J. Duffy, N. Harbeck, I.J. Christensen, and C. Thomssen Pooled analysis of prognostic impact of urokinase-type plasminogen activator and its inhibitor PAI-1 in 8377 breast cancer patients J Natl Cancer Inst 94 2002 116 128
7. T. Fuchs, and H. Allgayer Transcriptional regulation of the urokinase receptor (u-PAR) - a central molecule of invasion and metastasis Biol Chem 384 2003 755 761
8. E. Soravia, A. Grebe, P. De Luca, K. Helin, T.T. Suh, and J.L. Degen A conserved TATA-less proximal promoter drives basal transcription from the urokinase-type plasminogen activator receptor gene Blood 86 1995 624 635
9. H. Allgayer, H. Wang, G.E. Gallick, A. Crabtree, A. Mazar, and T. Jones Transcriptional induction of the urokinase receptor gene by a constitutively active Src. Requirement of an upstream motif (-152/-135) bound with Sp1 J Biol Chem 274 1999 18428 18437
10. A.R. Black, J.D. Black, and J. Azizkhan-Clifford Sp1 and kruppel-like factor family of transcription factors in cell growth regulation and cancer J Cell Physiol 188 2001 143 160
11. S.L. Samson, and N.C. Wong Role of Sp1 in insulin regulation of gene expression J Mol Endocrinol 29 2002 265 729
12. P. Bouwman, and S. Philipsen Regulation of the activity of Sp1-related transcription factors Mol Cell Endocrinol 195 2002 27 38
13. F. Borellini, Y.F. He, A. Aquino, G. Yu, S.F. Josephs, and R.I. Glazer Increased DNA binding and transcriptional activity associated with transcription factor Sp1 in K562 cells transfected with the myeloid-specific c-fes tyrosine kinase gene J Biol Chem 266 1991 15850 15854
14. S.J. Kim, U.S. Onwuta, Y.I. Lee, R. Li, M.R. Botchan, and P.D. Robbins The retinoblastoma gene product regulates Sp1-mediated transcription Mol Cell Biol 12 1992 2455 2463
15. S. Sif, A.J. Capobianco, and T.D. Gilmore The v-Rel oncoprotein increases expression from Sp1 site-containing promoters in chicken embryo fibroblasts Oncogene 8 1993 2501 2509
16. A. Zannetti, S. Del Vecchio, M.V. Carriero, R. Fonti, P. Franco, and G. Botti Coordinate up-regulation of Sp1 DNA-binding activity and urokinase receptor expression in breast carcinoma Cancer Res 60 2000 1546 1551
17. D.M. Schewe, J.H. Leupold, D.D. Boyd, E.R. Lengyel, H. Wang, and K.U. Gruetzner Tumor-specific transcription factor binding to an activator protein-2/Sp1 element of the urokinase-type plasminogen activator receptor promoter in a first large series of resected gastrointestinal cancers Clin Cancer Res 9 2003 2267 2276
18. I. Ibanez-Tallon, C. Ferrai, E. Longobardi, I. Facetti, F. Blasi, and M.P. Crippa Binding of Sp1 to the proximal promoter links constitutive expression of the human uPA gene and invasive potential of PC3 cells Blood 100 2002 3325 3332
19. E. Benasciutti, G. Pages, O. Kenzior, W. Folk, F. Blasi, and M.P. Crippa MAPK and JNK transduction pathways can phosphorylate Sp1 to activate the uPA minimal promoter elements and endogenous gene transcription Blood 104 2004 256 262
20. A. Ray, and B. Norden Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future Faseb J 14 2000 1041 1060
21. A. Romanelli, M. Saviano, and C. Pedone Synthetic strategies for obtaining PNA and PNA conjugates Recent Res Dev Org Chem 8 2004 237 254
22. J.D. Dignam, R.M. Lebovitz, and R.G. Roeder Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei Nucleic Acids Res 11 1983 1475 1489
23. J.E. Darnell Jr. Transcription factors as targets for cancer therapy Nat Rev Cancer 2 2002 740 749
24. M.J. Mann, and V.J. Dzau Therapeutic applications of transcription factor decoy oligonucleotides J Clin Invest 106 2000 1071 1075
25. Y.S. Cho, M.K. Kim, C. Cheadle, C. Neary, Y.G. Park, and K.G. Becker A genomic-scale view of the cAMP response element-enhancer decoy: a tumor target-based genetic tool Proc Natl Acad Sci USA 99 2002 15626 15631
26. R. Morishita, N. Tomita, Y. Kaneda, and T. Ogihara Molecular therapy to inhibit NFkappaB activation by transcription factor decoy oligonucleotides Curr Opin Pharmacol 4 2004 139 146
27. L. Penolazzi, E. Lambertini, M. Borgatti, R. Piva, M. Cozzani, and I. Giovannini Decoy oligodeoxynucleotides targeting NF-kappaB transcription factors: induction of apoptosis in human primary osteoclasts Biochem Pharmacol 66 2003 1189 1198
28. L.H. Wang, X.Y. Yang, X. Zhang, K. Mihalic, W. Xiao, and W.L. Farrar The cis decoy against the estrogen response element suppresses breast cancer cells via target disrupting c-fos not mitogen-activated protein kinase activity Cancer Res 63 2003 2046 2051
29. M. Borgatti, I. Lampronti, A. Romanelli, C. Pedone, M. Saviano, and N. Bianchi Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites J Biol Chem 278 2003 7500 7509
30. M. Borgatti, A. Romanelli, M. Saviano, C. Pedone, I. Lampronti, and L. Breda Resistance of decoy PNA-DNA chimeras to enzymatic degradation in cellular extracts and serum Oncol Res 13 2003 279 287
31. E. Uhlmann Peptide nucleic acids (PNA) and PNA-DNA chimeras: from high binding affinity towards biological function Biol Chem 379 1998 1045 1052
32. M.J. Mann, A.D. Whittemore, M.C. Donaldson, M. Belkin, M.S. Conte, and J.F. Polak Ex-vivo gene therapy of human vascular bypass grafts with E2F decoy: the PREVENT single-centre, randomised, controlled trial Lancet 354 1999 1493 1498
33. E.M. Novak, M. Metzger, R. Chammas, M. da Costa, K. Dantas, and C. Manabe Downregulation of TNF-alpha and VEGF expression by Sp1 decoy oligodeoxynucleotides in mouse melanoma tumor Gene Ther 10 2003 1992 1997
34. T. Kubo, N. Kinjyo, A. Ikezawa, T. Kambe, and R. Fukumori Sp1 decoy oligodeoxynucleotide decreases angiotensin receptor expression and blood pressure in spontaneously hypertensive rats Brain Res 992 2003 1 8
35. I. Kawamura, R. Morishita, S. Tsujimoto, T. Manda, M. Tomoi, and N. Tomita Intravenous injection of oligodeoxynucleotides to the NF-kappaB binding site inhibits hepatic metastasis of M5076 reticulosarcoma in mice Gene Ther 8 2001 905 912
36. L.M. Matrisian, G.W. Sledge Jr., and S. Mohla Extracellular proteolysis and cancer: meeting summary and future directions Cancer Res 63 2003 6105 6109
37. L.M. Coussens, B. Fingleton, and L.M. Matrisian Matrix metalloproteinase inhibitors and cancer: trials and tribulations Science 295 2002 2387 2392