Rebamipide significantly inhibits indomethacin-induced mitochondrial damage, lipid peroxidation, and apoptosis in gastric epithelial RGM-1 cells

Digestive Diseases and Sciences

Volumn 50, Issue SUPPL. 1, 2005, Pages

  Nagano, Yumiko ^a,b   Matsui, Hirofumi ^a,b   Muramatsu, Mutsumi ^a   Shimokawa, Osamu ^a,b   Shibahara, Takeshi ^a   Yanaka, Akinori ^a   Nakahara, Akira ^a   Matsuzaki, Yasushi ^a   Tanaka, Naomi ^a   Nakamura, Yukio ^b  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b RIKEN BIORESOURCE CENTER   (Japan)

Author keywords

Apoptosis; Fluorescence; Mitochondria; NSAIDs; ROS (reactive oxygen species)

Indexed keywords

CASPASE 3; CASPASE 9; FLUORESCENT DYE; HOESCHT 33258; INDOMETACIN; MITORED; REBAMIPIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DISORDERS OF MITOCHONDRIAL FUNCTIONS; DRUG EFFECT; DRUG MECHANISM; ENZYME ACTIVATION; EPITHELIUM CELL; FLUORESCENCE MICROSCOPE; LIPID PEROXIDATION; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT; STOMACH EPITHELIUM;

ALANINE; ANIMALS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; ANTI-ULCER AGENTS; APOPTOSIS; CELL CULTURE TECHNIQUES; FLUORESCENT DYES; GASTRIC MUCOSA; INDOMETHACIN; LIPID PEROXIDATION; MEMBRANE POTENTIALS; MITOCHONDRIA; QUINOLONES; RATS; RATS, WISTAR; REACTIVE OXYGEN SPECIES;

EID: 25644432016     PISSN: 01632116     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10620-005-2810-7     Document Type: Article

References (30)

1. Steinmeyer J: Pharmacological basis for the therapy of pain and inflammation with nonsteroidal anti-inflammatory drugs. Arthritis Res 2:379-385, 2000
2. Somasundaram S, Rafi S, Hayllar J, Sigthorsson G, Jacob M, Price AB, Macpherson A, Mahmod T, Scott D, Wrigglesworth JM, Bjarnason I: Mitochondrial damage: a possible mechanism of the "topical" phase of NSAID induced injury to the rat intestine. Gut 41:344-353, 1997
3. Davies NM, Saleh JY, Skjodt NM: Detection and prevention of NSAID-induced enteropathy. J Pharm Pharm Sci 3:137-155, 2000
4. Mahmud T, Rafi SS, Scott DL, Wrigglesworth JM, Bjarnason I: Nonsteroidal anti-inflammatory drugs and uncoupling of mitochondrial oxidative phosphorylation. Arthritis Rheum 39:1998-2003, 1996
5. Mingatto FE, dos Santos AC, Rodrigues T, Pigoso AA, Uyemura SA, Curti C: Effects of nimesulide and its reduced metabolite on mitochondria. Br J Pharmacol 131:1154-1160, 2000
6. Yoshida Y, Singh I, Darby CP: Effects of salicylic acid and calcium on mitochondrial functions. Acta Neurol Scand 85:191-196, 1992
7. 2+. Liver 14:103-108, 1994
8. Murphy MP, Smith RA: Drug delivery to mitochondria: the key to mitochondrial medicine. Adv Drug Deliv Rev 41:235-250, 2000
9. Arakawa T, Kobayashi K, Yoshikawa T, Tarnawski A: Rebamipide: overview of its mechanisms of action and efficacy in mucosal protection and ulcer healing. Dig Dis Sci 43(9 Suppl):5S-13S, 1998
10. Naito Y, Yoshikawa T, Tanigawa T, Sakurai K, Yamasaki K, Uchida M, Kondo M: Hydroxyl radical scavenging by rebamipide and related compounds: electron paramagnetic resonance study. Free Radic Biol Med 18:117-123, 1995
11. Iinuma S, Naito Y, Yoshikawa T, Takahashi S, Takemura T, Yoshida N, Kondo M: In vitro studies indicating antioxidative properties of rebamipide. Dig Dis Sci 43(9 Suppl):35S-39S, 1998
12. Naito Y, Yoshikawa T, Yoshida N, Kondo M: Role of oxygen radical and lipid peroxidation in indomethacin-induced gastric mucosal injury. Dig Dis Sci 43(9 Suppl):30S-34S, 1998
13. Naito Y, Yoshikawa T, Iinuma S, Yagi N, Matsuyama K, Boku Y, Fuji T, Yoshida N, Kondo M, Sasaki E: Rebamipide protects against indomethacin-induced gastric mucosal injury in healthy volunteers in a double blind, placebo-controlled study. Dig Dis Sci 43(9 Suppl):83S-89S, 1998
14. Watanabe S, Wang XE, Hirose M, Osada T, Tanaka H, Sato N: Rebamipide prevented delay of wound repair induced by hydrogen peroxide and suppressed apoptosis of gastric epithelial cells in vitro. Dig Dis Sci 43(9 Suppl):107S-112S, 1998
15. Matsui H, Murata Y, Hirano K, Sasaki T, Shiba R, Muto H, Ohno T: Hydrogen peroxide-induced cellular injury is associated with increase in endogenous fluorescence from rat gastric mucosal epithelial cell culture: a new method for detecting oxidative cellular injury by fluorescence measurement. J Gastroenterol 33:318-325, 1998
16. Kinoshita Y, Nakata H, Hassan S, Asahara M, Kawanami C, Matsushima Y, Maribayashi-Inomoto Y, Ping CY, Min D, Nakamura A, Chiba T: Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions. Gastroenterology 109:1068-1077, 1995
17. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K: A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red, and crystal violet. Biol Pharm Bull 19:1518-1520, 1996
18. Ishiyama K, Hayakawa T, Yamaya T: Expression of NADH-dependent glutamate synthase protein in the epidermis and exodermis of rice roots in response to the supply of ammonium ions. Planta 204:288-294, 1998
19. Okimotoa Y, Watanabea A, Nikia E, Yamashitab T, Noguchia N: A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett 474:137-140, 2000
20. Somasundaram S, Hayllar H, Rafi S, Wrigglesworth JM, Macpherson AJ, Bjarnason I: The biochemical basis of nonsteroidal anti-inflammatory drug-induced damage to the gastrointestinal tract: a review and a hypothesis. Scand J Gastroenterol 30:289-299, 1995
21. Slomiany BL, Piotrowski J, Slomiany A: Induction of tumor necrosis factor-alfa and apoptosis in gastric mucosal injury by indomethacin: effect of omeprazole and ebrotidine. Scand J Gastroenterol 32:638-642, 1997
22. Fuji Y, Matsura T, Kai M, Matsui H, Kawasaki H, Yamada K: Mitochondrial cytochrome-c release and caspase-3-like protease activation during indomethacin-induced apoptosis in rat gastric mucosal cells. Proc Soc Exp Biol Med 224:102-108, 2000
23. Murata Y, Matsui H, Hirano KI, Kondo Y, Yanaka A, Nakahara A, Tanaka N, Muto H: Autofluorescence in indomethacin-induced gastric mucosal lesions in rats. J Gastroenterol 35:510-517, 2000
24. Murata Y, Matsui H, Nakahara A, Tanaka N: Endoscopic fluorescence observation of gastric mucosa after indomethacin treatment in rats. Endoscopy 34:208, 2002
25. Jacob M, Bjarnason I, Simpson RJ: Effect of indomethacin on energy metabolism in jejunal tissue in vivo. Clin Sci 102:541-546, 2002
26. Varbiro G, Veres B, Gallyas F Jr, Sumegi B: Direct effect of Taxol on free radical formation and mitochondrial permeability transition. Free Radic Biol Med 3:548-558, 2001
27. Zhou XM, Wong BC, Fan XM, Zhang HB, Lin MC, Kung HF, Fan DM, Lam SK: Nonsteroidal anti-inflammatory drugs induce apoptosis in gastric cancer cells through upregulation of bax and bak. Cacinogenesis 22:1393-1397, 2001
28. Ogino K, Hobara T, Ishiyama H, Yamasaki K, Kobayashi H, Izumi Y, Oka S: Antiulcer mechanism of action of rebamipide, a novel antiulcer compound, on dethyldithicarbamate-induced antral gastric ulcer in rats. Eur J Pharmacol 212:9-13, 1992, Erratum in: Eur J Pharmacol 219:493, 1992
29. Basivireddy J, Vasudevan A, Jacob M, Balasubramanian KA: Indomethacin-induced mitochondrial dysfunction and oxidative stress in villous enterocytes. Biochem Pharmacol 64:339-349, 2002
30. Suzuki M, Miura S, Mori M, Kai A, Suzuki H, Fukumura D, Suematsu M, Tsuchiya M: Rebamipide, a novel antiulcer agent, attenuates Helicobacter pylori induced gastric mucosal injury associated with neutrophil-derived oxidants. Gut 35:1375-1378, 1994