A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet

Biological and Pharmaceutical Bulletin

Volumn 19, Issue 11, 1996, Pages 1518-1520

  Ishiyama, Munetaka ^a   Tominaga, Hideyuki ^a   Shiga, Masanobu ^a   Sasamoto, Kazumi ^a   Ohkura, Yosuke ^a   Ueno, Keiyu ^a  

^a Dojindo Laboratories   (Japan)

Author keywords

cell viability; combined assay; cytotoxicity; dehydrogenase; formazan; tetrazolium salt

Indexed keywords

CRYSTAL VIOLET; DODECYL SULFATE SODIUM; FORMAZAN; NEUTRAL RED; TETRAZOLIUM;

ARTICLE; ASSAY; CELL CULTURE; CELL VIABILITY; CHEMOSENSITIVITY; COLORIMETRY; CYTOTOXICITY; HELA CELL; HUMAN; HUMAN CELL; SOLUBILITY;

EID: 0029803056     PISSN: 09186158     EISSN: None     Source Type: Journal    
DOI: 10.1248/bpb.19.1518     Document Type: Article

References (12)

1. Mosmann T., J. Immunol. Methods, 65, 55-63 (1983).
2. Borenfreund E., Puerner J. A., J. Tiss. Cult. Meth., 9, 7-9 (1984): idem, Toxicol. Lett., 24, 119-124 (1985).
3. Borenfreund E., Puerner J. A., J. Tiss. Cult. Meth., 9, 7-9 (1984): idem, Toxicol. Lett., 24, 119-124 (1985).
4. Saotome K., Morita H., Umeda M., Toxic. in Vitro, 3, 317-321 (1989).
5. MTT forms a formazan which tends to precipitate within cells and. as a result, injures. cells.
6. Ishiyama M., Shiga M., Sasamoto K., Mizoguchi M., He P.-G., Chem. Pharm. Bull., 41, 1118-1122 (1993).
7. Ishiyama M., Tominaga H., Shiga M., Sasamoto K., Ohkura Y., Ueno K., Watanabe M., In Vitro Toxicology, 8, 187-190 (1995).
8. Yano T., Teruya K., Shirahata S., Watanabe J., Osada K., Tachibana H., Ohashi H., Kim E. H., Murakami H., Cytotechnology, 16, 167-178 (1994); Iwaki T., Iwaki A., Fukumaki Y., Tateishi J., Brain Res., 673, 47-52 (1995); Teruya K., Yano T., Shirahata S., Watanabe J., Osada K., Ohashi H., Tachibana H., Kim E. H., Murakami H., Biosci. Biotech. Biochem., 59, 341-344 (1995); Liu S. Q., Saijo K., Todoroki T., Ohno T., Nature Medicine, 1, 267-271 (1995); Takenouchi T., Munekata E., Life Sci., 56, 479-484 (1995).
9. Yano T., Teruya K., Shirahata S., Watanabe J., Osada K., Tachibana H., Ohashi H., Kim E. H., Murakami H., Cytotechnology, 16, 167-178 (1994); Iwaki T., Iwaki A., Fukumaki Y., Tateishi J., Brain Res., 673, 47-52 (1995); Teruya K., Yano T., Shirahata S., Watanabe J., Osada K., Ohashi H., Tachibana H., Kim E. H., Murakami H., Biosci. Biotech. Biochem., 59, 341-344 (1995); Liu S. Q., Saijo K., Todoroki T., Ohno T., Nature Medicine, 1, 267-271 (1995); Takenouchi T., Munekata E., Life Sci., 56, 479-484 (1995).
10. Yano T., Teruya K., Shirahata S., Watanabe J., Osada K., Tachibana H., Ohashi H., Kim E. H., Murakami H., Cytotechnology, 16, 167-178 (1994); Iwaki T., Iwaki A., Fukumaki Y., Tateishi J., Brain Res., 673, 47-52 (1995); Teruya K., Yano T., Shirahata S., Watanabe J., Osada K., Ohashi H., Tachibana H., Kim E. H., Murakami H., Biosci. Biotech. Biochem., 59, 341-344 (1995); Liu S. Q., Saijo K., Todoroki T., Ohno T., Nature Medicine, 1, 267-271 (1995); Takenouchi T., Munekata E., Life Sci., 56, 479-484 (1995).
11. Yano T., Teruya K., Shirahata S., Watanabe J., Osada K., Tachibana H., Ohashi H., Kim E. H., Murakami H., Cytotechnology, 16, 167-178 (1994); Iwaki T., Iwaki A., Fukumaki Y., Tateishi J., Brain Res., 673, 47-52 (1995); Teruya K., Yano T., Shirahata S., Watanabe J., Osada K., Ohashi H., Tachibana H., Kim E. H., Murakami H., Biosci. Biotech. Biochem., 59, 341-344 (1995); Liu S. Q., Saijo K., Todoroki T., Ohno T., Nature Medicine, 1, 267-271 (1995); Takenouchi T., Munekata E., Life Sci., 56, 479-484 (1995).
12. Yano T., Teruya K., Shirahata S., Watanabe J., Osada K., Tachibana H., Ohashi H., Kim E. H., Murakami H., Cytotechnology, 16, 167-178 (1994); Iwaki T., Iwaki A., Fukumaki Y., Tateishi J., Brain Res., 673, 47-52 (1995); Teruya K., Yano T., Shirahata S., Watanabe J., Osada K., Ohashi H., Tachibana H., Kim E. H., Murakami H., Biosci. Biotech. Biochem., 59, 341-344 (1995); Liu S. Q., Saijo K., Todoroki T., Ohno T., Nature Medicine, 1, 267-271 (1995); Takenouchi T., Munekata E., Life Sci., 56, 479-484 (1995).