Energy Transfer Spectroscopy for Measuring Mitochondrial Metabolism in Living Cells

Photochemistry and Photobiology

Volumn 66, Issue 1, 1997, Pages 34-41

  Schneckenburger, Herbert ^a,b   Gschwend, Michael H ^a   Strauss, Wolfgang S L ^a   Sailer, Reinhard ^a   Kron, Martina ^a   Steeb, Ulrike ^a   Steiner, Rudolf ^a  

^a UNIVERSITY OF ULM   (Germany)

^b Fachhochschule Aalen   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE; ROTENONE;

ANIMAL; AORTA; ARTICLE; CATTLE; CELL LINE; DRUG EFFECT; ENERGY TRANSFER; KINETICS; LIGHT; METABOLISM; METHODOLOGY; MITOCHONDRION; QUANTUM THEORY; RADIATION EXPOSURE; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY; VASCULAR ENDOTHELIUM;

ANIMALS; AORTA; CATTLE; CELL LINE; ENDOTHELIUM, VASCULAR; ENERGY TRANSFER; KINETICS; LIGHT; MITOCHONDRIA; NAD; QUANTUM THEORY; ROTENONE; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE;

EID: 0031178870     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-1097.1997.tb03135.x     Document Type: Article

References (41)

1. DiMauro, S., E. Bonilla, M. Zeviani, M. Nakagawa and D. C. DeVivo (1985) Mitochondrial myopathies. Ann. Neurol. 17, 521-526.
2. Capaldi, R. A. (1988) Mitochondrial myopathies and respiratory chain proteins. Trends Biochem. Sci. 13, 144-148.
3. Wallace, D. C. (1992) Diseases of the mitochondrial DNA. Annu. Rev. Biochem. 61, 1175-1212.
4. Luft, R. (1994) The development of mitochondrial medicine. Proc. Natl. Acad. Sci. USA 91, 8731-8738.
5. Jackson, M. J., J. A. Schaefer, M. A. Johnson, A. A. M. Morris, D. M. Turnbull and L. A. Bindoff (1995) Presentation and clinical investigation of respiratory chain disease: a study of 51 patients. Brain 118, 339-357.
6. Schapira, A. H. V. (1994) Evidence for mitochondrial dysfunction in Parkinson's disease - a critical appraisal. Movement Disorders 9, 125-138.
7. Parker, W. D., N. J. Mahr, C. M. Filley, J. K. Parks, D. Hughes, D. A. Young and C. M. Cullum (1994) Reduced platelet cytochrome c oxidase activity in Alzheimer's disease. Neurology 44, 1086-1090.
8. Hasitz, M., Z. Rácz, A. Nagy and A. Lipcsey (1995) Importance of platelet function in Alzheimer's disease. Arch. Gerontol. Geriatr. 21, 53-61.
9. Cleeter, M. W. J., J. M. Cooper, V. M. Darley-Usmar, S. Moncada and A. H. V. Schapira (1994) Reversible inhibition of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, by nitric oxide: implications for neurodegenerative diseases. FEBS Lett 345, 50-54.
10. Smith, P. R., J. M. Cooper, G. G. Govan, E. A. Harding and A. H. V. Schapira (1993) Smoking and mitochondrial function: a model for environmental toxins. Q. J. Med. 86, 657-660.
11. Udenfriend, S. (1962) Fluorescence Assay in Biology and Medicine. Academic Press, New York.
12. Galeotti, T., G. D. V. VanRossum, D. H. Mayer and B. Chance (1970) On the fluorescence of NAD(P)H in whole cell preparations of tumours and normal tissues. Eur. J. Biochem. 17, 485-496.
13. Salmon, J.-M., E. Kohen, P. Viallet, J. G. Hirschberg, A. W. Wouters, C. Kohen and B. Thorell (1982) Microspectrofluorometric approach to the study of free/bound NAD(P)H ratio as metabolic indicator in various cell types. Photochem. Photobiol. 36, 585-593.
14. Nokubo, M., I. Zs-Nagy, K. Kitani and M. Ohta (1988) Characterization of the autofluorescence of rat liver plasma membranes. Biochim. Biophys. Acta 939, 441-448.
15. Galland, P. and H. Senger (1988) The role of flavins as photoreceptors. J. Photochem. Photobiol. B Biol. 1, 277-294.
16. Horvath, K. A., D. F. Torchiana, W. M. Daggett and N. S. Nishioka (1992) Monitoring myocardial reperfusion injury with NADH fluorometry. Lasers Surg. Med. 12, 2-6.
17. Horvath, K. A., K. T. Shomacker, C. C. Lee and L. H. Cohn (1994) Intraoperative myocardial ischemia detection with laser-induced fluorescence. J. Thorac. Cardiovasc. Surg. 107, 220-225.
18. Obi-Tabot, E. T., L. M. Hanrahan, R. Cachecho, E. R. Berr, S. R. Hopkins, J. C. K. Chan, J. M. Shapiro and W. W. LaMorte (1993) Changes in hepatocyte NADH fluorescence during prolonged hypoxia. J. Surg. Res. 55, 575-580.
19. Lohmann, W. and E. Paul (1988) In situ detection of melanomas by fluorescence measurements. Naturwissenschaften 75, 201-202.
20. Scott, T. G., R. D. Spencer, N. J. Leonard and G. Weber (1970) Emission properties of NADH. Studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, and simplified synthetic models. J. Am. Chem. Soc. 92, 687-695.
21. + bound to dehydrogenases. Biochemistry 14, 362-368.
22. Lakowicz, J. R. (1983) Principles of Fluorescence Spectroscopy. Plenum Press, New York.
23. Schneckenburger, H. and K. König (1992) Fluorescence decay kinetics and imaging of NAD(P)H and flavins as metabolic indicators. Opt. Eng. 31, 1447-1451.
24. Couprie, M. E., F. Mérola, P. Tauc, D. Garzella, A. Delboulbé, T. Hara and M. Billardon (1994) First use of the uv Super-ACO free electron laser: fluorescence decay and rotational dynamics of the NADH coenzyme. Rev. Sci. Instrum. 65, 1485-1495.
25. Bernarding, J., A. Napiwotzki and H.-D. Kronfeldt (1996) Time-resolved fluorescence of endogenous metabolites for the early detection of tumours; part 1: NADH and NADPH. Lasermedizin 12, 27-33.
26. Paul, R.-J. and H. Schneckenburger (1996) Oxygen concentration and the oxidation-reduction state of yeast: determination of free/bound NADH and flavins by time-resolved spectroscopy. Naturwissenschaften 83, 32-35.
27. Förster, T. (1960) Zwischenmolekularer Übergang von Elektronen-Anregungsenergie. Z. Elektrochem. 64, 157-165.
28. Uster, P. S. and R. E. Pagano (1986) Resonance energy transfer microscopy: observation of membrane-bound fluorescent probes in model membranes and in living cells. J. Cell Biol. 103, 1221-1234.
29. Szöllösi, J., S. Damjanovich, S. A. Mulhern and L. Tron (1987) Fluorescence energy transfer and membrane potential measurements monitor dynamic properties of cell membranes: a critical review. Prog. Biophys. Mol. Biol. 49, 65-87.
30. Squier, T. C., D. J. Bigelow, J. G. DeAncos and G. Inesi (1987) Localization of site-specific probes on the Ca-ATPase of sarcoplasmic reticulum using fluorescence energy transfer. J. Biol. Chem. 262, 4748-4754.
31. Johnson, L. V., M. L. Walsh and L. B. Chen (1980) Localization of mitochondria in living cells with rhodamine 123. Proc. Natl. Acad. Sci. USA 77, 990-994.
32. Horikoshi, T., T. Yoshioka, Y. Kubota and K. Yanagisawa (1987) Fluorescent dye monitoring of mitochondrial changes associated with malignant cell transformation. Cell Struct. Funct. 12, 525-537.
33. Port, H., H. Schneckenburger and H. C. Wolf (1981) Host-guest energy transfer via dipole-dipole interaction in doped fluorene crystals. Z. Naturforsch. 36a, 697-704.
34. Halle, W., W.-E. Siems, K. D. Jentzsch, E. Teuscher und E. Görres (1984) Die in vitro kultivierte Aorten-Endothelzelle in der Wirkstofforschung - Zellphysiologische Charakterisierung und Einsatzmöglichkeiten der Zellinie BKEz-7. Die Pharmazie 39, 77-81.
35. Tzagaloff, A. (1982) Mitochondria. Plenum Press, New York.
36. Schneckenburger, H., W. Strauß, A. Rück, H. K. Seidlitz and J. Wessels (1992) Microscopic fluorescence spectroscopy and diagnosis. Opt. Eng. 31, 995-999.
37. Schneckenburger, H., K. König, T. Dienersberger and R. Hahn (1994) Time-gated microscopic imaging and spectroscopy in medical diagnosis and photobiology. Opt. Eng. 33, 2600-2606.
38. Högel, J., J. Schmid and W. Gaus (1994) Robustness of the standard deviation and other measures of dispersion. Biom. J. 36, 411-427.
39. Kubin, R. F. and A. N. Fletcher (1982) Fluorescence quantum yield of some rhodamine dyes. J. Luminesc. 27, 455-462.
40. Johnson, L. V., M. L. Walsh, B. J. Bockus and L. B. Chen (1982) Monitoring of relative mitochondrial membrane potential in living cell by fluorescence microscopy. J. Cell Biol. 88, 526-535.
41. Ronot, X., L. Benel, M. Adolphe and J.-C. Mounolou (1986) Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry. Biol. Cell. 57, 1-8.