Kinetic and structural analysis of active site mutants of monofunctional NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae

Biochemistry

Volumn 44, Issue 39, 2005, Pages 13163-13171

  Wagner, Wendi ^a   Breksa III, Andrew P ^a,b   Monzingo, Arthur F ^a   Appling, Dean R ^a   Robertus, Jon D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CARBON; ENZYME KINETICS; MUTAGENESIS; OXIDATION;

HYDRIDE TRANSFER; ONE-CARBON UNITS; OXIDATION STATES;

ENZYMES;

AMINO ACID; FOLIC ACID; GLUTAMIC ACID; GLUTAMINE; LYSINE; METHYLENETETRAHYDROFOLATE DEHYDROGENASE; METHYLENETETRAHYDROFOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXIDOREDUCTASE; THREONINE; THYMIDINE PHOSPHATE; TYROSINE;

ARTICLE; CATALYSIS; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; KINETICS; MUTAGENESIS; OXIDATION; PRIORITY JOURNAL; PURINE SYNTHESIS; SACCHAROMYCES CEREVISIAE; STEADY STATE; STRUCTURE ANALYSIS;

BINDING SITES; FOLIC ACID; KINETICS; METHYLENETETRAHYDROFOLATE DEHYDROGENASE (NAD+); MOLECULAR STRUCTURE; MUTATION; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE PROTEINS; SUBSTRATE SPECIFICITY;

SACCHAROMYCES CEREVISIAE;

EID: 25444467164     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051038x     Document Type: Article

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