Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: Roles for aspartate 120 and glutamate 28

Biochemistry

Volumn 40, Issue 21, 2001, Pages 6216-6226

  Trimmer, Elizabeth E ^a,b   Ballou, David P ^a   Ludwig, Martha L ^a   Matthews, Rowena G ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b GRINNELL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; CRYSTAL STRUCTURE; ENZYMES; ESCHERICHIA COLI;

ELECTROSTATIC STABILIZATION;

CATALYSIS;

5,10 METHYLENETETRAHYDROFOLATE REDUCTASE (FADH2); ASPARTIC ACID; BACTERIAL ENZYME; FLAVOPROTEIN; FOLIC ACID; GLUTAMIC ACID; MUTANT PROTEIN;

ARTICLE; CATALYSIS; CHEMICAL REACTION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; ASPARAGINE; ASPARTIC ACID; CATALYSIS; ENZYME ACTIVATION; ESCHERICHIA COLI; FOLIC ACID; GLUTAMIC ACID; GLUTAMINE; KINETICS; METHYLENETETRAHYDROFOLATE DEHYDROGENASE (NAD+); METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH2); MUTAGENESIS, SITE-DIRECTED; NAD; NAD(P)H DEHYDROGENASE (QUINONE); OXIDATION-REDUCTION; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; SPECTROPHOTOMETRY; VITAMIN K;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035967514     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi002790v     Document Type: Article

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