Characterization of a highly purified, fully active, crystallizable RC-LH1-PufX core complex from Rhodobacter sphaeroides

Photosynthesis Research

Volumn 86, Issue 1-2, 2005, Pages 61-70

  Abresch, E C ^a   Axelrod, H L A ^a,b   Beatty, J T ^c   Johnson, J A ^c   Nechushtai, R ^d   Paddock, M L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b SLAC NATIONAL ACCELERATOR LABORATORY   (United States)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Bacterial photosynthesis; Core complex; Light harvesting; PufX; RC LH1; Reaction center

Indexed keywords

BACTERIAL PROTEIN; PUFX PROTEIN, RHODOBACTER;

CHEMISTRY; CONFERENCE PAPER; CRYSTALLIZATION; ISOLATION AND PURIFICATION; LIGHT HARVESTING SYSTEM; METABOLISM; PROTEIN BINDING; RHODOBACTER SPHAEROIDES; SPECTROSCOPY;

BACTERIAL PROTEINS; CRYSTALLIZATION; LIGHT-HARVESTING PROTEIN COMPLEXES; PROTEIN BINDING; RHODOBACTER SPHAEROIDES; SPECTRUM ANALYSIS;

BACTERIA (MICROORGANISMS); PHOTOBACTERIA; RHODOBACTER SPHAEROIDES;

EID: 25444464841     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11120-005-5106-z     Document Type: Conference Paper

References (42)

1. EC Abresch HLA Axelrod JT Beatty JA Johnson MY Okamura G Feher R Nechushtai 2004 Isolation and characterization of an active RCLH1 complex from Rhodobacter sphaeroides Biophys J 86 1 146a
2. S Bahatyrova RN Frese CA Siebert JD Olsen KO van der Werf R van Grondelle RA Niederman PA Bullough C Otto CN Hunter 2004a The native architecture of a photosynthetic membrane Nature 430 158 162
3. S Bahatyrova RN Frese KO van der Werf C Otto CN Hunter JD Olsen 2004b Flexibility and size heterogeneity of the LH1 light harvesting complex revealed by atomic force microscopy: functional significance for bacterial photosynthesis J Biol Chem 279 21327 21333
4. WP Barz A Vermeglio F Francia G Venturoli BA Melandri D Oesterhelt 1995 Role of the PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 2.PufX is required for efficient ubiquinone/ubiquinol exchange between the reaction center QB site and the cytochrome bc1 complex Biochemistry 34 15248 15258
5. RE Blankenship MT Madigan CE Bauer 1995 Anoxygenic Photosynthetic Bacteria Vol. 2. Kluwer Academic Publishers Dordrecht, The Netherlands
6. RM Broglie CN Hunter P Delepaire RA Niederman C Nam-Hai RK Clayton 1980 Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresies Proc Natl Acad Sci USA 77 87 91
7. X-Y Chen V Yurkov ML Paddock MY Okamura JT Beatty 1998 A puhA gene deletion and plasmid complementation system for facile site directed mutagenesis studies of the reaction center H protein of Rhodobacter sphaeroides Photosynth Res 55 369 373
8. HM Cho LJ Mancino RE Blankenship 1984 Light saturation curves and quantum yields in reaction centers from photosynthetic bacteria Biophys J 45 455 461
9. RK Clayton 1966 Spectroscopic analysis of bacteriochlorophylls in vitro and in vivo Photochem Photobiol 5 669 677
10. RJ Cogdell JG Lindsay J Valentine Durant 1982 A further characterization of the B890 light-harvesting pigment-protein complex from Rhodospirillum rubrum strain S-1 FEBS Lett 150 151 154
11. JW Farchaus WP Barz H Gruenberg D Oesterhelt 1992 Studies on the expression of the pufX polypeptide and its requirement for photoheterotrophic growth in Rhodobacter sphaeroides EMBO J 11 8 2779 2788
12. A Fejes EC Yi DR Goodlett JT Beatty 2003 Shotgun proteomic analysis of chromatophores from the purple phototrophic bacterium Rhodopseudomonas palustris Photosynth Res 78 195 203
13. - charge separation in reaction centers of Rhodobacter sphaeroides Biochemisty 43 14199 14210
14. F Francia J Wang G Venturoli BA Melandri WP Barz D Oesterhelt 1999 The reaction center-LH1 antenna complex of Rhodobacter sphaeroides contains one PufX molecule which is involved in dimerization of this complex Biochemistry 38 6834 6845
15. C Francke J Amesz 1995 The size of the photosynthetic unit in purple bacteria Photosynth Res 46 347 352
16. X Hu T Ritz 2002 Photosynthetic apparatus of purple bacteria Quat Rev Biophys 35 1 1 62
17. X Hu K Schulten 1998 Model for the light-harvesting complex I (B875) of Rhodobacter sphaeroides Biophys J 75 683 694
18. I Ikeda-Yamaski T Odahara K Mitsuoka Y Fujiyoshi K Murata 1998 FEBS Lett 425 505 508
19. S P Jamieson Wang P Qian J Kirkland J Conroy C Hunter P Bullough 2002 Projection structure of the photosynthetic reaction center-antenna complex of Rhodospirillum rubrum at 8.5 Å resolution EMBO J 21 3927 3935
20. C Jungas J Ranck J Rigaud P Joliot A Vermeglio 1999 Supramolecular organization of the photosynthetic apparatus of Rhodobacter sphaeroides EMBO J 18 534 542
21. S Karrasch P Bullough R Ghosh 1995 The 8.5 Å projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits EMBO J 14 631 638
22. B Biochim Biophys Acta 766 126 140
23. B Biochim Biophys Acta 766 126 140
24. F Kleinherenbrink G Deinum S Otte A Hoff J Amesz 1992 Energy transfer from long-wavelength absorbing bacteriochlorophylls to the reaction center Biochim Biophys Acta 1099 175 181
25. TG Lilburn CE Haith RC Prince JT Beatty 1992 Pleiotropic effects of pufX gene deletion on the structure and function of the photosynthetic apparatus of Rhodobacter capsulatus Biochim Biophys Acta 1100 160 170
26. SW Meinhardt PJ Kiley S Kaplan AR Crofts S Harayama 1985 Characterization of light-harvesting mutants of Rhodopseudomonas sphaeroides. I. Measurement of the efficiency of energy transfer from light harvesting complexes to the reaction center Arch Biochem Biophys 236 1 130 139
27. MY Okamura ML Paddock MS Graige G Feher 2000 Proton and electron transfer in bacterial reaction centers Biochim Biophys Acta 1458 148 163
28. PS Parkes-Loach CJ Law PA Recchia J Kehoe S Nehrlich J Chen PA Loach 2001 Role of the core region of the PufX protein in inhibition of reconstitution of the core light-harvesting complexes of Rhodobacter sphaeroides and Rhodobacter capsulatus Biochemistry 40 5593 5601
29. P Qian T Yagura Y Koyama R Cogdell 2000 Isolation and purification of the reaction center (RC) and the core (RC-LH1) complex from Rhodium marinum: the LH1 ring of the detergent-solubulized core complex contains 32 bacteriochlorophylls Plant Cell Physiol 41 1347 1353
30. A Roszak T Howard J Southall A Gardiner C Law N Isaacs R Cogdell 2003 Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris Science 302 1969 1972
31. J Sambrook EF Fritsch T Maniatis 1989 Molecular Cloning: a Laboratory Manual Cold Spring Harbor Laboratory Press Plainview, New York
32. S Scheuring J Seguin S Marco D Levy B Robert J Rigaud 2003 Nanodissection and high-resolution imaging of the Rhodpseudomonas viridis photosynthetic core complex in native membranes by AFM PNAS 100 1690 1693
33. S Scheuring F Francia J Busselez B Melandri J Rigaud D Levy 2004 Structural role of PufX in the dimerization of the photosynthetic core complex of Rhodobacter sphaeroides J Biol Chem 279 3620 3626
34. S Scheuring J Busselez D Lévy 2005 Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ AFM JBC 280 1426 1431
35. CA Siebert P Qian D Fotiadis A Engel CN Hunter PA Bullough 2004 Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX EMBO J 23 4 690 700
36. SC Straley WW Parson DC Mauzerall RK Clayton 1973 Pigment content and molar extinction coefficients of photochemical reaction centers from Rhodopseudomonas spheroids Biochim Biophys Acta 305 597 609
37. A Tehrani JT Beatty 2004 Effects of precise deletions in Rhodobacter sphaeroides reaction center genes on steady-state levels of reaction center proteins: a revised model for reaction center assembly Photosynth Res 79 101 108
38. R Theiler F Suter JD Pennoyer H Zuber RA Niederman 1985 Complete amino acid sequence of the B875 light-harvesting protein of Rhodopseudomonas sphaeroides strain 2.4.1. Comparison with R26.1 carotenoidless-mutant strain FEBS Lett 184 2 231 236
39. T Ueda Y Morimoto M Sato T Kakuno J Yamashita T Horio 1985 Isolation, characterization and comparison of a ubiquitous pigment-protein complex consisting of a reaction center and light-harvesting bacteriochlorophyll proteins present in purple photosynthetic bacteria J Biochem 98 6 1487 1498
40. M Van der Rest G Gingras 1974 The pigment complement of the photosynthetic reaction center isolated from Rhodospirillum rubrum J Biol Chem 249 20 6446 6453
41. T Walz SJ Jamieson CM Bowers CA Bullough CN Hunter 1998 Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 Å, LH1 and RC-LH1 at 25 Å J Mol Biol 282 833 845
42. JC Williams LA Steiner G Feher 1986 Primary structure of the reaction center from Rhodopseudomonas sphaeroides Proteins 1 4 312 325