Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides: LH2 at 6 Å, LH1 and RC-LH1 at 25 Å

Journal of Molecular Biology

Volumn 282, Issue 4, 1998, Pages 833-845

  Walz, Thomas ^a   Jamieson, Stuart J ^a   Bowers, Claire M ^a   Bullough, Per A ^a   Hunter, C Neil ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

2 D crystals; Electron crystallography; Light harvesting complexes; Membrane protein; Photosynthesis

Indexed keywords

BACTERIAL PROTEIN; PROTEIN SUBUNIT;

ARTICLE; BACTERIAL MEMBRANE; CRYSTAL STRUCTURE; NONHUMAN; PHOTOSYNTHESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RHODOBACTER SPHAEROIDES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; RHODOBACTER SPHAEROIDES; RHODOPSEUDOMONAS; RHODOSPIRILLUM RUBRUM;

EID: 0032475815     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.2050     Document Type: Article

References (47)

1. Amos L.A., Henderson R., Unwin P.N.T. Three-dimensional structure determination by electron microscopy of two-dimensional crystals. Prog. Biophys. Mol. Biol. 39:1982;183-231
2. Allen J.P., Feher G., Yeates T.O., Komya H., Rees D.C. Structure of the reaction centre from Rhodobacter sphaeroides R-26 the cofactors. Proc. Natl Acad. Sci. USA. 84:1987;5730-5734
3. Barz W.P., Francia F., Venturoli G., Melandri B.A., Vermigelio A., Oesterhelt D. Role of PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 1. PufX is required for efficient light-driven electron transfer and photophosphorylation under anaerobic conditions. Biochemistry. 34:1995;15235-15247
4. 1 complex. Biochemistry. 34:1995;15248-15258
5. Bullough P.A., Henderson R. Phase accuracy in high-resolution electron-microscopy of trigonal and orthorhombic purple membrane. Biophys. J. 58:1990;705-711
6. Bullough P.A., Tulloch P.A. High-resolution spot-scan electron-microscopy of microcrystals of an alpha-helical coiled-coil protein. J. Mol. Biol. 215:1990;161-173
7. Chang C.-H., Tiede D., Tang J., Smith U., Norris J., Schiffer M. Structure of Rhodopseudomonas sphaeroides R-26 reaction centre. FEBS Letters. 205:1986;82-86
8. Cogdell R.J., Fyfe P.K., Barrett S.J., Prince S., Freer A.A., Isaacs N.W., Hunter C.N. The purple bacterial photosynthetic unit. Photosynth. Res. 48:1996;55-63
9. Crowther R.A., Henderson R., Smith J.M. MRC image processing programs. J. Struct. Biol. 116:1996;9-16
10. Deisenhofer J., Epp O., Miki K., Huber R., Michel H. X-ray structure analysis at 3 Å resolution of a membrane protein complex folding of the protein subunits in the photosynthetic reaction centre from Rhodopseudomonas viridis. Nature. 318:1985;618-624
11. Engelhardt H., Engel A., Baumeister W. Stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris. Proc. Natl Acad. Sci. USA. 83:1986;8972-8976
12. Farchaus J.W., Grünberg H., Oesterhelt D. Complementation of a reaction centre-deficient Rhodobacter sphaeroides pufLMX deletion strain in trans with pufBALM does not restore the photo-synthetic-positive phenotype. J. Bacteriol. 172:1990;977-985
13. Farchaus J.W., Barz W.P., Grünberg H., Oesterhelt D. Studies on the expression of the pufX polypeptide and its requirement for photoheterotrophic growth in Rhodobacter sphaeroides. EMBO J. 11:1992;2779-2788
14. Fowler G.J.S., Hess S., Sundström V., Hunter C.N. The role of βArg-10 in the B800 bacteriochlorophyll and carotenoid pigment environment within the light harvesting LH2 complex of Rhodobacter sphaeroides. Biochemistry. 36:1997;11282-11291
15. Ghosh R., Ghosh-Eicher S., Di Berardino M., Bachofen R. Isolation and characterization of a B875 protein kinase from Rhodospirillum rubrum. Biochim. Biophys. Acta. 1184:1994;28-36
16. Grigorieff N., Ceska T.A., Downing K.H., Baldwin J.M., Henderson R. Electron-crystallographic refinement of the structure of bacteriorhodopsin. J. Mol. Biol. 259:1996;393-421
17. Henderson R., Baldwin J.M., Downing K.H., Lepault J., Zemlin F. Structure of purple membrane from Halobacterium halobium recording, measurement and evaluation of electron micrographs at 3.5 Å resolution. Ultramicroscopy. 19:1986;147-178
18. Henderson R., Baldwin J.M., Ceska T.A., Zemlin F., Beckmann E., Downing K.H. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213:1990;899-929
19. Hoenger A., Pagès J.-M., Fourel D., Engel A. The orientation of porin OmpF in the outer membrane of Escherichia coli. J. Mol. Biol. 233:1993;400-413
20. Hunter C.N., Olsen J., van Grondelle R. Photosynthetic antenna proteins 100 ps before photosynthesis starts. Trends Biochem. Sci. 14:1989;72-76
21. Ikeda-Yamasaki I., Odahara T., Mitsuoka K., Fujiyoshi Y., Murata K. Projection map of the reaction center-light harvesting 1 complex from Rhodopseudomonas viridis at 10 Å resolution. FEBS Letters. 425:1998;505-508
22. Jap B.K., Zulauf M., Scheybani T., Hefti A., Baumeister W., Aebi U., Engel A. 2D crystallization from art to science. Ultramicroscopy. 46:1992;45-84
23. Jones M.R., Fowler J.G.S., Gibson L.C.D., Grief G.G., Olsen J.D., Crielaard W., Hunter C.N. Construction of mutants of Rhodobacter sphaeroides lacking one or more pigment-protein complexes and complementation with reaction centre, LH1 and LH2 genes. Mol. Microbiol. 6:1992;1173-1184
24. Karrasch S., Bullough P.A., Ghosh R. The 8.5 Å projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. EMBO J. 14:1995;631-638
25. Kessi J., Ghosh R., Bachofen R. Purification of an LHI-RC-complex of Rhodospirillum rubrum by solubilization of chromatophores with a short-chain lecithin. Photosynth. Res. 46:1995;353-362
26. Kimura Y., Vassylyev D.G., Miyazawa A., Kidera A., Matsushima M., Mitsuoka K., Murata K., Hirai T., Fujiyoshi Y. Surface structure of bacteriorhodopsin revealed by high resolution electron crystallography. Nature. 389:1997;206-211
27. Koepke J., Hu X., Muenke C., Schulten K., Michel H. The crystal structure of the light-harvesting complex II (B800-850) from Rhodospirillum molischianum. Structure. 4:1996;581-597
28. Kühlbrandt W., Wang D.N., Fujiyoshi Y. Atomic model of plant light-harvesting complex by electron crystallography. Nature. 367:1994;614-621
29. Lilburn T.G., Haith C.E., Prince R.C., Beatty J.T. Pleiotropic effects of pufX gene deletion on the structure and function of the photosynthetic apparatus of Rhodobacter capsulatus. Biochim. Biophys. Acta. 110:1992;160-170
30. McDermott G., Prince S.M., Freer A.A., Hawthornthwaite-Lawlee A.M., Papiz M.Z., Cogdell R.J., Isaacs N.W. Crystal structure of of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature. 374:1995;517-525
31. McGlynn P., Hunter C.N., Jones M.R. The Rhodobacter sphaeroides Puf X protein is not required for photosynthetic competence in the absence of a light harvesting system. FEBS Letters. 349:1994;349-353
32. McGlynn P., Westerhuis W.H.J., Jones M.R., Hunter C.N. Consequences for the organisation of reaction centre light harvesting antenna 1 (LH1) core complexes of Rhodobacter sphaeroides arising from the deletion of amino acid residues from the C terminus of the LH1 α polypeptide. J. Biol. Chem. 271:1996;3285-3292
33. Miller K.R. Three-dimensional structure of a photosynthetic membrane. Nature. 300:1982;53-55
34. Monger T., Parson W.W. Singlet-triplet fusion in Rhodopseudomonas sphaeroides chromatophores. Biochim. Biophys. Acta. 460:1977;393-407
35. Montoya G., Cyrklaff M., Sinning I. Two-dimensional crystallization and preliminary structure analysis of light harvesting II (B800-850) complex from the purple bacterium Rhodovulum sulfidophilum. J. Mol. Biol. 250:1995;1-10
36. Nogales E., Wolf S.G., Zhang S.X., Downing K.H. Preservation of 2-D crystals of tubulin for electron crystallography. J. Struct. Biol. 115:1995;199-208
37. Olsen J.D., Sockalingum G.D., Robert B., Hunter C.N. Modification of a hydrogen bond to a bacteriochlorophyll a molecule in the light-harvesting 1 antenna of Rhodobacter sphaeroides. Proc. Natl Acad. Sci. USA. 91:1994;7124-7128
38. Olsen J.D., Sturgis J., Hunter C.N., Robert B. Site-directed modification of ligands to the bacteriochlorophylls of the light harvesting LH1 and LH2 complexes of Rhodobacter sphaeroides. Biochemistry. 36:1997;12625-12632
39. Savage H., Cyrklaff M., Montoya G., Kühlbrandt W., Sinning I. Two-dimensional structure of light harvesting complex II (LHII) from the purple bacterium Rhodovulum sulfidophilum and comparison with LHII from Rhodopseudomonas acidophila. Structure. 4:1996;243-252
40. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:1987;368-379
41. Stahlberg H., Dubochet J., Vogel H., Ghosh R. Are light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction centre in a square geometry? J. Mol. Biol. 282:1998;819-831
42. Stark W., Kühlbrandt W., Wildhaber H., Wehrli E., Muhlethaler K. The structure of the photoreceptor unit of Rhodopseudomonas viridis. EMBO J. 3:1984;777-783
43. Sturgis J., Olsen C.N., Robert B., Hunter C.N. The functions of conserved tryptophan residues of the core light harvesting complex of Rhodobacter sphaeroides. Biochemistry. 36:1997;2772-2778
44. Valpuesta J.M., Carrascosa J.L., Henderson R. Analysis of electron microscope images and electron diffraction patterns of thin crystals of Φ29 connectors in ice. J. Mol. Biol. 240:1994;281-287
45. Vos M., van Grondelle R., van der Kooi F.W., van der Poll D., Amesz J., Duysens L.N.M. Singlet-singlet annihilation at low teperatures in the antenna of purple bacteria. Biochim. Biophys. Acta. 850:1986;501-512
46. Walz T., Ghosh R. Two-dimensional crystallization of the light-harvestingI-reaction centre photounit from Rhodospirillum rubrum. J. Mol. Biol. 265:1997;107-111
47. Woodbury N.W., Allen J.P. The pathway, kinetics and thermodynamics of electron transfer in wild type and mutant reaction centers of purple nonsulfur bacteria. Blankenship R.E., Madigan M.T., Bauer C.E. The Anoxygenic Photosynthetic Bacteria. 1995;Kluwer, Dordrecht