A puhA gene deletion and plasmid complementation system for facile site directed mutagenesis studies of the reaction center H protein of Rhodobacter sphaeroides

Photosynthesis Research

Volumn 55, Issue 2-3, 1998, Pages 369-373

  Chen, X Y ^a   Yurkov, V ^a   Paddock, M L ^b   Okamura, M Y ^b   Beatty, J T ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Complementation; Deletion; puhA Gene; Reaction center H protein; Rhodobacter sphaeroides; Site directed mutagenesis

Indexed keywords

BACTERIA (MICROORGANISMS); PROKARYOTA; RHODOBACTER SPHAEROIDES;

EID: 0031856624     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1005983903558     Document Type: Conference Paper

References (19)

1. Alberti M, Burke DE and Hearst JE (1995) Structure and sequence of the photosynthetic gene cluster. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Phosynthetic Bacteria. Kluwer Academic Publishers, Dordrecht, the Netherlands
2. Beatty JT and Gest H (1981) Generation of succinyl-coenzyme A in photosynthetic bacteria. Arch Microbiol 129: 335-340
3. Bérard J and Gingras G (1991) The puh structural gene coding for the H subunit of the Rhodospirillum rubrum photoreaction center. Biochem Cell Biol 69: 122-131
4. Keen NT, Tamaki S, Kobayashi D and Trollinger D (1988) Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197
5. Lancaster CRD, Ermler U and Michel H (1995) The structures of photosynthetic: reaction centers from purple bacteria as revealed by X-ray crystallography. In: Blankenship RE, Madigan MT an Bauer CE (eds) Anoxygenic Photosynthetic Bacteria. Kluwer Academic Publishers, Dordrecht, the Netherlands
6. LeBlanc HN and Beatty JT (1993) Rhodobacter capsulatus puc operon: Promoter Location, transcript sizes and effects of deletions on photosynthetic growth. J. Gen Microbiol, 139: 101-109
7. LeBlanc HN and Beatty JT (1996) Topological analysis of the Rhodobacter capsulatus PucC protein and effects of C-terminal deletions on light-harvesting complex II. J Bacteriol 178: 4801-4806
8. Lilburn TG, Prince RC and Beatty JT (1995) Mutation of the Ser2 codon of the light-harvesting B870 α polypeptide of Rhodobacter capsulatus partially suppresses the pufX phenotype. J Bacteriol 177: 4593-4600
9. B in reaction centers from photosynthetic bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria. Kluwer Academic Publishers, Dordrecht, the Netherlands
10. Prentki P and Krisch HM (1984) In vitro insertional mutagenesis with a selectable DNA fragment. Gene 9: 303-313
11. Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
12. Schägger H and von Jagow H (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379
13. Simon R, Pricfer U and Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1: 37-45
14. Sockett RE, Donghue TJ, Varga AR and. Kaplan S (1989) Control of photosynthetic membrane assembly in Rhodobacter sphaeroides mediated by puhA and flanking sequences. J Bacteriol 171: 436-446
15. Stowell MH, Mc Phillips TM, Rees DC, Soltis SM, Abresch E and Feher G (1997) Light-induced structural changes in photosynthetic reaction center: implications for mechanism of electron-proton transfer. Science 276: 812-816
16. Takahashi E and Wraight CA (1996) Potentiation of proton transfer function by electrostatic interactions in photosynthetic reaction centers from Rhodobacter sphaeroides: First results from site-direct mutation of the H subunit. Proc Natl Acad Sci USA 93: 2640-2645
17. Williams JC and Taguchi AKW (1995) Genetic manipulation of purple photosynthetic bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria. Kluwer Academic Publishers, Dordrecht, the Netherlands
18. Wong DK-H, Collins WJ, Harmer A, Lilburn TG and Beatty JT (1996) Directed, mutagenesis of the Rhodobacter capsulatus puHA gene and pleiotropic effects on photosynthetic reaction center and light-harvesting I complexes. J Bacteriol 178: 2334-2342
19. Woodbury NW and Allen JP (1995) The pathway, kinetics and thermodynamics of electron transfer in wild type and mutant reaction centers of purple nonsulfur bacteria. In: Blankenship RE Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria. Kluwer Academic Publishers, Dordrecht, the Netherlands