Iodo-α-conotoxin MI selectively binds the α/δ subunit interface of muscle nicotinic acetylcholine receptors

Biochemistry

Volumn 43, Issue 21, 2004, Pages 6656-6662

  Luo, Siqin ^a   McIntosh, J Michael ^a,b  

^a UNIVERSITY OF UTAH   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; MUSCLE; PROTEINS; SYNTHESIS (CHEMICAL);

EMBYRONIC MUSCLE; LIGAND BINDING;

BIOCHEMISTRY;

ALPHA BUNGAROTOXIN; CONOTOXIN; IODO ALPHA CONOTOXIN MI; ION CHANNEL; LIGAND; NICOTINIC ACETYLCHOLINE RECEPTOR ALPHA1; NICOTINIC ACETYLCHOLINE RECEPTOR BETA1; NICOTINIC ACETYLCHOLINE RECEPTOR DELTA; NICOTINIC ACETYLCHOLINE RECEPTOR GAMMA; NICOTINIC RECEPTOR; RECEPTOR SUBUNIT; TUBOCURARINE CHLORIDE; TYROSINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL FREE SYSTEM; CONTROLLED STUDY; EMBRYO; FETUS; GENE EXPRESSION; KINETICS; LIGAND BINDING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RADIOIODINATION; SYNTHESIS; XENOPUS;

ANIMALS; BINDING SITES; BINDING, COMPETITIVE; BUNGAROTOXINS; CELLS, CULTURED; CONOTOXINS; FEMALE; HUMANS; IODINE; IODINE RADIOISOTOPES; KINETICS; MICE; MUSCLE, SKELETAL; NICOTINIC ANTAGONISTS; OOCYTES; PROTEIN SUBUNITS; RECEPTORS, NICOTINIC; TUBOCURARINE; TYROSINE; XENOPUS;

ANIMALIA;

EID: 2542581752     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049906y     Document Type: Article

References (28)

1. Chiappinelli, V. A. (1993) Chapter 3. Neurotoxins acting on acetylcholine receptors, in Natural and Synthetic Neurotoxins (Harvey, A., Ed.) pp 65-109, Academic Press, Harcourt Brace Jovanovich, London.
2. Antil-Delbeke, S., Gaillard, C., Tamiya, T., Corringer, P. J., Changeux, J. P., Servent, D., and Menez, A. (2001) Molecular determinants by which a long chain toxin from snake venom interacts with the neuronal α7-nicotinic acetylcholine receptor, J. Biol. Chem. 275, 29594-29601.
3. McIntosh, J. M. (2000) Toxin antagonists of the neuronal nicotinic acetylcholine receptor, in Handbook of Experimental Pharmacology. Volume 144. Neuronal Nicotinic Receptors (Clementi, F., Fornasari, D., and Gotti, C., Eds.) pp 455-476, Springer-Verlag, Berlin, Germany.
4. Blount, P., and Merlie, J. P. (1989) Molecular basis of the two nonequivalent ligand binding sites of the muscle nicotinic acetylcholine receptor, Neuron 3, 349-357.
5. Sine, S. M. (1993) Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of residues that determine curare selectivity, Proc. Natl. Acad. Sci. U.S.A. 90, 9436-9440.
6. McArdle, J. J., Lentz, T. L., Witzemann, V., Schwarz, H., Weinstein, S. A., and Schmidt, J. J. (1999) Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor, J. Pharmacol. Exp. Ther. 289, 543-550.
7. Taylor, P., Malanz, S., Molles, B. E., Osaka, H., and Tsigelny, I. (2000) Subunit interface selective toxins as probes of nicotinic acetylcholine receptor subtypes, Pflugers Arch. 440 (Suppl. 5), R115-R117.
8. Kreienkamp, H. J., Sine, S. M., Maeda, R. K., and Taylor, P. (1994) Glycosylation sites selectively interface with α-toxin binding to the nicotinic acetylcholine receptor, J. Biol. Chem. 269, 8108-8114.
9. Groebe, D. R., Dumm, J. M., Levitan, E. S., and Abramson, S. N. (1995) α-Conotoxins selectively inhibit one of the two acetylcholine binding sites of nicotinic receptors, Mol. Pharmacol. 48, 105-111.
10. Sine, S. M., Kreienkamp, H.-J., Bren, N., Maeda, R., and Taylor, P. (1995) Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of determinants of α-conotoxin MI selectivity, Neuron 15, 205-211.
11. Johnson, D. S., Martinez, J., Elgoyhen, A. B., Heinemann, S. F., and McIntosh, J. M. (1995) α-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric α7 and α9 receptors, Mol. Pharmacol. 48, 194-199.
12. Pedersen, S. E., and Cohen, J. B. (1990) d-Tubocurarine binding sites are located at α-γ and α-δ subunit interfaces of the nicotinic acetylcholine receptor, Proc. Natl. Acad. Sci. U.S.A. 87, 2785-2789.
13. Chiara, D. C., Xie, Y., and Cohen, J. B. (1999) Structure of the agonist-binding sites of the Torpedo nicotinic acetylcholine receptor: affinity-labeling and mutational analyses identify γTyr-111/ δArg-113 as antagonist affinity determinants, Biochemistry 38, 6689-6698.
14. Luo, S., Nguyen, T. A., Cartier, G. E., Olivera, B. M., Yoshikami, D., and McIntosh, J. M. (1999) Single-residue alteration in α-conotoxin PnIA switches its nAChR subtype selectivity, Biochemistry 38, 14542-14548.
15. Cartier, G. E., Yoshikami, D., Gray, W. R., Luo, S., Olivera, B. M., and McIntosh, J. M. (1996) A new α-conotoxin which targets α3β2 nicotinic acetylcholine receptors, J. Biol. Chem. 271, 7522-7528.
16. Jacobsen, R. B., DelaCruz, R. G., Grose, J. H., McIntosh, J. M., Yoshikami, D., and Olivera, B. M. (1999) Critical residues influence the affinity and selectivity of α-conotoxin MI for nicotinic acetylcholine receptors, Biochemistry 38, 13310-13315.
17. Gray, W. R., Rivier, J. E., Galyean, R., Cruz, L. J., and Olivera, B. M. (1983) Conotoxin MI: Disulfide bonding and conformational states, J. Biol. Chem. 258, 12247-12251.
18. Lukas, R. J. (1986) Characterization of curaremimetic neurotoxin binding sites on membrane fractions derived from the human medulloblastroma clonal line, TE671, J. Neurochem. 46, 1936-1941.
19. Luther, M. A., Schoepfer, R., Whiting, P., Casey, B., Blatt, Y., Montal, M. S., Montal, M., and Lindstrom, J. (1989) A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastroma cell line TE671, J. Neurosci. 9, 1082-1096.
20. Jacobsen, R., Yoshikami, D., Ellison, M., Martinez, J., Gray, W. R., Cartier, G. E., Shon, K. J., Groebe, D. R., Abramson, S. N., Olivera, B. M., and McIntosh, J. M. (1997) Differential targeting of nicotinic acetylcholine receptors by novel αA-conotoxins, J. Biol. Chem. 272, 22531-22537.
21. Sine, S. M. (1988) Functional properties of human skeletal muscle acetylcholine receptors expressed by the TE671 cell line, J. Biol. Chem. 163, 18052-18062.
22. Lukas, R. J. (1986) Immunochemical and pharmacological distinctions between curaremimetic neurotoxin binding sites of central, autonomic, and peripheral origin, Proc. Natl. Acad. Sci. U.S.A. 83, 5741-5745.
23. Conroy, W. G., Saedi, M. S., and Lindstrom, J. (1990) TE671 cells express an abundance of a partially mature acetylcholine receptor α subunit which has characteristics of an assembly intermediate, J. Biol. Chem. 265, 21642-21651.
24. Bencherif, M., Eisenhour, C. M., Prince, R. J., Lippiello, P. M., and Lukas, R. J. (1995) The "calcium antagonist" TMB-8 [3,4,5-trimethoxybenzoic acid 9-(diethylamino)octyl ester] is a potent, noncompetitive, functional antagonist at diverse nicotinic acetylcholine receptor subtypes, J. Pharmacol. Exp. Ther. 275, 1418-1426.
25. Sugiyama, N., Marchot, P., Kawanishi, C., Osaka, H., Molles, B., Sine, S. M., and Taylor, P. (1998) Residues at the subunit interfaces of the nicotinic acetylcholine receptor that contribute to α-conotoxin MI binding, Mol. Pharmacol. 53, 787-794.
26. Bren, N., and Sine, S. M. (2000) Hydrophobic pairwise interactions stabilize α-conotoxin MI in the muscle acetylcholine receptor binding site, J. Biol. Chem. 275, 12692-12700.
27. Papineni, R. V. L., Sanchez, J. U., Baksi, K., Willcockson, I. U., and Pedersen, S. E. (2001) Site-specific charge interactions of a-conotoxin MII with the nicotinic acetylcholine receptor, J. Biol. Chem. 276, 23589-23598.
28. Gouda, H., Yamazaki, K., Hasegawa, J., Kobayashi, Y., Nishiucni, Y., Sakakibara, S., and Hirono, S. (1997) Solution structure of α-conotoxin MI determined by 1H-NMR spectroscopy and molecular dynamics simulation with the explicit solvent water, Biochim. Biophys. Acta 1343, 327-334.