Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies

Journal of Histochemistry and Cytochemistry

Volumn 52, Issue 6, 2004, Pages 723-733

  Sweetwyne, Mariya T ^a,c   Brekken, Rolf A ^a,d   Workman, Gail ^a   Bradshaw, Amy D ^a,e   Carbon, Juliet ^a   Siadak, Anthony W ^a,f   Murri, Carrie ^a   Sage, E Helene ^a,b  

^a HOPE HEART INSTITUTE   (United States)

^b BENAROYA RESEARCH INSTITUTE   (United States)

^c University of Alabama at Birmingham   (United States)

^d UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^e MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^f ZYMOGENETICS INC   (United States)

Author keywords

Collagen; Extracellular matrix; Matricellular; Monoclonal antibody; SPARC

Indexed keywords

CELL PROTEIN; COLLAGEN TYPE 1; COLLAGEN TYPE 3; EPITOPE; GLYCOPROTEIN; GROWTH FACTOR; LAMININ 1; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 293; OSTEONECTIN; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CELL ADHESION; CELL INTERACTION; CELL PROLIFERATION; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; HOMEOSTASIS; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; TISSUE REPAIR; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; EXTRACELLULAR MATRIX PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MICE; ORGAN SPECIFICITY; OSTEONECTIN; PRECIPITIN TESTS; PROTEIN BINDING;

EID: 2542554186     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.3A6153.2004     Document Type: Article

References (32)

1. Bolander ME, Robey PG, Fisher LW, Conn KM, Prabhakar BS, Termine JD (1989) Monoclonal antibodies against osteonectin show conservation of epitopes across species. Calcif Tissue Int 45:74-80
2. Bornstein P, Sage EH (2002) Matricellular proteins: extracellular modulators of cell function. Curr Opin Cell Biol 14:608-616
3. Bradshaw AD, Bassuk JA, Francki A, Sage EH (2000) Expression and purification of recombinant human SPARC produced by baculovirus. Mol Cell Biol Res Commun 3:345-351
4. Bradshaw AD, Reed MJ, Carbon JG, Pinney E, Brekken RA, Sage EH (2001) Increased fibrovascular invasion of subcutaneous polyvinyl alcohol sponges in SPARC-null mice. Wound Repair Regen 9:522-530
5. Bradshaw AD, Reed MJ, Sage EH (2002) SPARC-null mice exhibit accelerated cutaneous wound closure. J Histochem Cytochem 50:1-10
6. Brekken RA, Overholser JP, Stastny VA, Waltenberger J, Minna JD, Thorpe PE (2000) Selective inhibition of vascular endothelial growth factor (VEGF) receptor 2 (KDR/Flk-1) activity by a monoclonal anti-VEGF antibody blocks tumor growth in mice. Cancer Res 60:5117-5124
7. Brekken RA, Puolakkainen P, Graves DC, Workman G, Lubkin SR, Sage EH (2003) Enhanced growth of tumors in SPARC null mice is associated with changes in the ECM. J Clin Invest 111:487-495
8. Brekken RA, Sage EH (2001) SPARC, a matricellular protein: at the crossroads of cell-matrix communication. Matrix Biol 19:816-827
9. Funk SE, Sage EH (1991) The Ca2(+)-binding glycoprotein SPARC modulates cell cycle progression in bovine aortic endothelial cells. Proc Natl Acad Sci USA 88:2648-2652
10. Funk SE, Sage EH (1993) Differential effects of SPARC and cationic SPARC peptides on DNA synthesis by endothelial cells and fibroblasts. J Cell Physiol 154:53-63
11. Hohenester E, Engel J (2002) Domain structure and organisation in extracellular matrix proteins. Matrix Biol 21:115-128
12. Holland PW, Harper SJ, McVey JH, Hogan BL (1987) In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. J Cell Biol 105:473-482
13. Iruela-Arispe ML, Lane TF, Redmond D, Reilly M, Bolender RP, Kavanagh TJ, Sage EH (1995) Expression of SPARC during development of the chicken chorioallantoic membrane: evidence for regulated proteolysis in vivo. Mol Biol Cell 6:327-343
14. Kearney JF, Radbruch A, Liesegang B, Rajewsky K (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol 123:1548-1550
15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
16. Lane RD (1985) A short-duration polyethylene glycol fusion technique for increasing production of monoclonal antibody-secreting hybridomas. J Immunol Methods 81:223-228
17. Lane TF, Iruela-Arispe ML, Johnson RS, Sage EH (1994) SPARC is a source of copper-binding peptides that stimulate angiogenesis. J Cell Biol 125:929-943
18. Lane TF, Sage EH (1994) The biology of SPARC, a protein that modulates cell-matrix interactions. FASEB J 8:163-173
19. Maillard C, Malaval L, Delmas PD (1992) Immunological screening of SPARC/osteonectin in nonmineralized tissues. Bone 13:257-264
20. Malaval L, Darbouret B, Preaudat C, Jolu JP, Delmas PD (1991) Intertissular variations in osteonectin: a monoclonal antibody directed to bone osteonectin shows reduced affinity for platelet osteonectin. J Bone Miner Res 6:315-323
21. Nomura S, Wills AJ, Edwards DR, Heath JK, Hogan BL (1988) Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol 106:441-450
22. Porter PL, Sage EH, Lane TF, Funk SE, Gown AM (1995) Distribution of SPARC in normal and neoplastic human tissue. J Histochem Cytochem 43:791-800
23. Puolakkainen P, Bradshaw AD, Kyriakides TR, Reed M, Brekken R, Wight T, Bornstein P (2003) Compromised production of extracellular matrix in mice lacking secreted protein, acidic and rich in cysteine (SPARC) leads to a reduced foreign body reaction to implanted biomaterials. Am J Pathol 162:627-635
24. Sage H, Vernon RB, Decker J, Funk S, Iruela-Arispe ML (1989a) Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice. J Histochem Cytochem 37:819-829
25. Sage H, Vernon RB, Funk SE, Everitt EA, Angello J (1989b) SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J Cell Biol 109:341-356
26. Sage EH, Reed M, Funk SE, Truong T, Steadele M, Puolakkainen P, Maurice DH (2003) Cleavage of the matricellular protein SPARC by matrix metalloproteinase 3 produces polypeptides that influence angiogenesis. J Biol Chem 278:37849-37857
27. Sasaki T, Gohring W, Mann K, Maurer P, Hohenester E, Knauper V, Murphy G (1997) Limited cleavage of extracellular matrix protein BM-40 by matrix metalloproteinases increases its affinity for collagens. J Biol Chem 272:9237-9243
28. Soderling JA, Reed MJ, Corsa A, Sage EH (1997) Cloning and expression of murine SC1, a gene product homologous to SPARC. J Histochem Cytochem 45:823-835
29. Villarreal XC, Malaval L, Mann KG, Delmas P, Long GL (1991) Epitope mapping of two monoclonal antibodies to the central portion of human osteonectin. Calcif Tissue Int 48:138-141
30. Yan Q, Blake D, Clark JI, Sage EH (2003) Expression of the matricellular protein SPARC in murine lens. SPARC is necessary for the structural integrity of the capsular basement membrane. J Histochem Cytochem 51:503-511
31. Yan Q, Clark JI, Wight TN, Sage EH (2002) Alterations in the lens capsule contribute to cataractogenesis in SPARC-null mice. J Cell Sci 115:2747-2756
32. Yost JC, Sage EH (1993) Specific interaction of SPARC with endothelial cells is mediated through a carboxyl-terminal sequence containing a calcium-binding EF hand. J Biol Chem 268:25790-25796