Src SH3/2 domain-mediated peripheral accumulation of Src and phospho-myosin is linked to deregulation of E-cadherin and the epithelial-mesenchymal transition

Molecular Biology of the Cell

Volumn 15, Issue 6, 2004, Pages 2794-2803

  Avizienyte, Egle ^a   Fincham, Valerie J ^a   Brunton, Valerie G ^a   Frame, Margaret C ^a,b  

^a BEATSON INSTITUTE FOR CANCER RESEARCH   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRIN; MITOGEN ACTIVATED PROTEIN KINASE; MYOSIN; MYOSIN LIGHT CHAIN KINASE; PHOSPHOMYOSIN; PROTEIN SH2; PROTEIN SH3; PROTEIN TYROSINE KINASE; RHO KINASE; SIGNALING LYMPHOCYTE ACTIVATION MOLECULE ASSOCIATED PROTEIN; UNCLASSIFIED DRUG; UVOMORULIN; VIMENTIN;

ANIMAL CELL; ARTICLE; CANCER CELL; CELL ADHESION; CELL CONTACT; COLON CANCER; COMPLEX FORMATION; CONTROLLED STUDY; DRUG ACCUMULATION; EPITHELIUM CELL; FIBROBLAST; MESENCHYME CELL; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SRC HOMOLOGY DOMAIN;

CADHERINS; CELL ADHESION; CELL DIFFERENTIATION; CELL LINE, TUMOR; COLONIC NEOPLASMS; EPITHELIAL CELLS; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; HUMANS; INTEGRINS; MAP KINASE SIGNALING SYSTEM; MESODERM; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MYOSIN-LIGHT-CHAIN KINASE; MYOSINS; PHOSPHORYLATION; POINT MUTATION; PROTO-ONCOGENE PROTEINS PP60(C-SRC); SERUM; SRC HOMOLOGY DOMAINS; TRANSFECTION; VIMENTIN;

ANIMALIA;

EID: 2542426492     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E03-12-0879     Document Type: Article

References (30)

1. Avizienyte, E., Wyke, A.W., Jones, R.J., McLean, G.W., Westhoff, M.A., Brunton, V.G., and Frame, M.C. (2002). Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling. Nat. Cell Biol. 4, 632-638.
2. Bibbins, K.B., Boeuf, H., and Varmus, H.E. (1993). Binding of the Src SH2 domain to phosphopeptides is determined by residues in both the SH2 domain and the phosphopeptides. Mol. Cell. Biol. 13, 7278-7287.
3. Bowman, T. et al. (2001). Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis. Proc. Natl. Acad. Sci. USA 98, 7319-7324.
4. Boyer, B., Roche, S., Denoyelle, M., and Thiery, J.P. (1997). Src and Ras are involved in separate pathways in epithelial cell scattering. EMBO J. 16, 5904-5913.
5. Boyer, B., Bourgeois, Y., and Poupon, M.F. (2002). Src kinase contributes to the metastatic spread of carcinoma cells. Oncogene 21, 2347-2356.
6. Braga, V.M., Machesky, L.M., Hall, A., and Hotchin, N.A. (1997). The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. J. Cell Biol. 137, 1421-1431.
7. Brahmbhatt, A.A., and Klemke, R.L. (2003). ERK and RhoA differentially regulate pseudopodia growth and retraction during chemotaxis. J. Biol. Chem. 278, 13016-13025.
8. Erpel, T., Superti-Furga, G., and Courtneidge, S.A. (1995). Mutational analysis of the Src SH3 domain: the same residues of the ligand binding surface are important for intra- and intermolecular interactions. EMBO J. 14, 963-975.
9. Fincham, V.J., Brunton, V.G., and Frame, M.C. (2000). The SH3 domain directs acto-myosin-dependenttargetingofv-Srctofocaladhesionsviaphosphatidylinositol 3-kinase. Mol. Cell. Biol. 20, 6518-6536.
10. Fukata, M., and Kaibuchi, K. (2001). Rho-family GTPases in cadherin-mediated cell-cell adhesion. Nat. Rev. Mol. Cell. Biol. 2, 887-897.
11. Garcia, R. et al. (2001). Constitutive activation of Stat3 by the Src and JAK tyrosine kinases participates in growth regulation of human breast carcinoma cells. Oncogene 20, 2499-2513.
12. Gupta, S.K., Gallego, C., Johnson, G.L., and Heasley, L.E. (1992). MAP kinase is constitutively activated in gip2 and src transformed rat la fibroblasts. J. Biol. Chem. 267, 7987-7990.
13. Jones, R.J., Avizienyte, E., Wyke, A.W., Owens, D.W., Brunton, V.B., and Frame, M.C. (2002). Elevated c-Src is linked to altered cell-matrix adhesion rather than proliferation in KM12C human colorectal cancer cells. Br. J. Cancer 87, 1128-1135.
14. Jou, T.S., and Nelson, W.J. (1998). Effects of regulated expression of mutant RhoA and Rac1 small GTPases on the development of epithelial (MDCK) cell polarity. J. Cell Biol. 142, 85-100.
15. Kelemen, B.R., Hsiao, K., and Goueli, S.A. (2002). Selective in vivo inhibition of mitogen-activated protein kinase activation using cell-permeable peptides. J. Biol. Chem. 277, 8741-8748.
16. Kirschmann, D.A., Seftor, E.A., Nieva, D.R., Mariano, E.A., and Hendrix, M.J. (1999). Differentially expressed genes associated with the metastatic phenotype in breast cancer. Breast Cancer Res. Treat. 55, 127-136.
17. Klemke, R.L., Cai, S., Giannini, A.L., Gallagher, P.J., de Lanerolle, P., and Cheresh, D.A. (1997). Regulation of cell motility by mitogen-activated protein kinase. J. Cell Biol. 137, 481-492.
18. Mansour, S.J., Matten, W.T., Hermann, A.S., Candia, J.M., Rong, S., Fukasawa, K., Vande Woude, G.F., and Ahn, N.G. (1994). Transformation of mammalian cells by constitutively active MAP kinase kinase. Science 265, 966-970.
19. Mayer, B.J., Jackson, P.K., Van Etten, R.A., and Baltimore, D. (1992). Point mutations in the abl SH2 domain coordinately impair phosphotyrosine binding in vitro and transforming activity in vivo. Mol. Cell. Biol. 12, 609-618.
20. Morikawa, K., Walker, S.M., Nakajima, M., Pathak, S., Jessup, J.M., and Fidler, I.J. (1988). Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice. Cancer Res. 48, 6863-6871.
21. Noren, N.K., Niessen, C.M., Gumbiner, B.M., and Burridge, K. (2001). Cadherin engagement regulates Rho family GTPases. J. Biol. Chem. 276, 33305-33308.
22. Noren, N.K., Arthur, W.T., and Burridge, K. (2003). Cadherin engagement inhibits RhoA via p190RhoGAP. J. Biol. Chem. 278, 13615-13618.
23. Penuel, E., and Martin, G.S. (1999). Transformation by v-Src: Ras-MAPK and PI3K-mTOR mediate parallel pathways. Mol. Biol. Cell 10, 1693-1703.
24. Sahai, E., and Marshall, C.J. (2002). ROCK and Dia have opposing effects on adherens junctions downstream of Rho. Nat. Cell Biol. 4, 408-415.
25. Takaishi, K., Sasaki, T., Kotani, H. Nishioka, H., and Takai, Y. (1997). Regulation of cell-cell adhesion by rac and rho small G proteins in MDCK cells. J. Cell Biol. 139, 1047-1059.
26. Thiery, J.P. (2002). Epithelial-mesenchymal transitions in tumour progression. Nat. Rev. Cancer 2, 442-454.
27. Turkson, J. et al. (2001). Phosphotyrosyl peptides block Stat3-mediated DNA binding activity, gene regulation, and cell transformation. J. Biol. Chem. 276, 45443-45455.
28. Webb, D.J., Donais, K., Whitmore, L.A., Thomas, S.M., Turner, C.E., Parsons, J.T., and Horwitz, A.F. (2004). FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nat. Cell Biol. 6, 154-161.
29. Wakatsuki, T., Wysolmerski, R.B., and Elson, E.L. (2003). Mechanics of cell spreading: role of myosin II. J. Cell Sci. 116, 1617-1625.
30. Zhong, C., Kinch, M.S., and Burridge, K. (1997). Rho-stimulated contractility contributes to the fibroblastic phenotype of Ras-transformed epithelial cells. Mol. Biol. Cell 8, 2329-2344.