Identification of Glycopeptide-resistant enterococci by VITEK 2 system and conventional and real-time polymerase chain reaction

Diagnostic Microbiology and Infectious Disease

Volumn 53, Issue 1, 2005, Pages 17-21

  Eisner, Alexandra ^a   Gorkiewicz, Gregor ^a   Feierl, Gebhard ^a   Leitner, Eva ^a   Köfer, Josef ^b   Kessler, Harald H ^a   Marth, Egon ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b Department of Veterinary Administration in Styria ^*  (Austria)

Author keywords

Glycopetide resistant enterococci; Multiplex PCR; Real time PCR; Vancomycin; VITEK 2

Indexed keywords

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; AUTOANALYSIS; AUTOMATION; BACTERIUM IDENTIFICATION; CONFERENCE PAPER; ENTEROCOCCUS; GLYCOPEPTIDE RESISTANT ENTEROCOCCUS; INTERMETHOD COMPARISON; MICROBIOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SPECIES IDENTIFICATION;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DRUG RESISTANCE, MICROBIAL; ENTEROCOCCUS; EVALUATION STUDIES; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; VANCOMYCIN; VANCOMYCIN RESISTANCE;

EID: 25144431751     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2005.04.001     Document Type: Conference Paper

References (26)

1. J. Barry, A. Brown, V. Ensor, U. Lakhani, D. Petts, C. Warren, and T. Winstanley Comparative evaluation of the VITEK 2 Advanced Expert System (AES) in five UK hospitals J. Antimicrob. Chemother. 51 2003 1191 1202
2. S. Chang, D.M. Sievert, J.C. Hageman, M.L. Boulton, F.C. Tenover, F.P. Downes, S. Shah, J.T. Rudrik, G.R. Pupp, W.J. Brown, D. Cardo, and S.K. Fridkin Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene N. Engl. J. Med. 348 2003 1342 1347
3. Y.S. Chen, S.A. Marshall, P.L. Winokur, S.L. Coffman, W.W. Wilke, P.R. Murray, C.A. Spiegel, M.A. Pfaller, G.V. Doern, and R.N. Jones Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci J. Clin. Microbiol. 36 1998 2996 3001
4. S. Dutka-Malen, S. Evers, and P. Courvalin Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR J. Clin. Microbiol. 33 1995 24 27
5. S. Elsayed, N. Hamilton, D. Boyd, and M. Mulvey Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp J. Clin. Microbiol. 39 2001 2367 2368
6. H.P. Endtz, N. van Den Braak, A. van Belkum, W.H. Goessens, D. Kreft, A.B. Stroebel, and H.A. Verbrugh Comparison of eight methods to detect vancomycin resistance in enterococci J. Clin. Microbiol. 36 1998 592 594
7. R. Facklam, and D.F. Sahm Enterococcus P.R. Murray E.J. Baron M.A. Pfaller F.C. Tenover R.H. Yolken Manual of clinical microbiology 1995 American Society of Microbiology Washington, DC 308 314
8. A.C. Fluit, M.R. Visser, and F.J. Schmitz Molecular detection of antimicrobial resistance Clin. Microbiol. Rev. 14 2001 836 871
9. F. Garcia-Garrote, E. Cercenado, and E. Bouza Evaluation of a new system, VITEK 2, for identification and antimicrobial susceptibility testing of enterococci J. Clin. Microbiol. 38 2000 2108 2111
10. G. Gorkiewicz, G. Feierl, R. Zechner, and E.L. Zechner Transmission of Campylobacter hyointestinalis from a pig to a human J. Clin. Microbiol. 40 2002 2601 2605
11. M.K. Hayden Insights into the epidemiology and control of infection with vancomycin-resistant enterococci Clin. Infect. Dis. 31 2000 1058 1065
12. M.K. Hayden, G.M. Trenholme, J.E. Schultz, and D.F. Sahm In vivo development of teicoplanin resistance in a vanB Enterococcus faecium isolate J. Infect. Dis. 167 1993 1224 1227
13. P. Jayaratne, and C. Rutherford Detection of clinically relevant genotypes of vancomycin-resistant enterococci in nosocomial surveillance specimens by PCR J. Clin. Microbiol. 37 1999 2090 2092
14. E.M. Jochimsen, L. Fish, K. Manning, S. Young, D.A. Singer, R. Baker, and W.R. Jarvis Control of vancomycin-resistant enterococci at a community hospital: efficacy of patient and staff cohorting Infect. Control. Hosp. Epidemiol. 20 1999 106 109
15. R. Kariyama, R. Mitsuhata, J.W. Chow, D.B. Clewell, and H. Kumon Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci J. Clin. Microbiol. 38 2000 3092 3095
16. H.H. Kessler, G. Muhlbauer, E. Stelzl, E. Daghofer, B.I. Santner, and E. Marth Fully automated nucleic acid extraction: MagNA Pure LC Clin. Chem. 47 2001 1124 1126
17. P.C. Kohner, R. Patel, J.R. Uhl, K.M. Garin, M.K. Hopkins, L.T. Wegener, and F.R. Cockerill III Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated Vitek methods for testing susceptibilities of Enterococcus spp. to vancomycin J. Clin. Microbiol. 35 1997 3258 3263
18. M. Ligozzi, C. Bernini, M.G. Bonora, M. De Fatima, J. Zuliani, and R. Fontana Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci J. Clin. Microbiol. 40 2002 1681 1686
19. National Committee for Clinical Laboratory Standards Performance standards for antimicrobial susceptibility testing: 13th informational supplement Document M100-S13 2003 NCCLS Wayne, PA
20. S. Palladino, I.D. Kay, A.M. Costa, E.J. Lambert, and J.P. Flexman Real-time PCR for the rapid detection of vanA and vanB genes Diagn. Microbiol. Infect. Dis. 45 2003 81 84
21. S. Palladino, I.D. Kay, J.P. Flexman, I. Boehm, A.M. Costa, E.J. Lambert, and K.J. Christiansen Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay J. Clin. Microbiol. 41 2003 2483 2486
22. A.K. Petrich, K.E. Luinstra, D. Groves, M.A. Chernesky, and J.B. Mahony Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR Mol. Cell. Probes 13 1999 275 281
23. L.M. Sloan, J.R. Uhl, E.A. Vetter, C.D. Schleck, W.S. Harmsen, J. Manahan, R.L. Thompson, J.E. Rosenblatt, and F.R. Cockerill III Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs J. Clin. Microbiol. 42 2004 2636 2643
24. F.C. Tenover, J.M. Swenson, C.M. O'Hara, and S.A. Stocker Ability of commercial and reference antimicrobial susceptibility testing methods to detect vancomycin resistance in enterococci J. Clin. Microbiol. 33 1995 1524 1527
25. N. van Den Braak, W. Goessens, A. van Belkum, H.A. Verbrugh, and H.P. Endtz Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci J. Clin. Microbiol. 39 2001 351 353
26. C.J. Whitener, S.Y. Park, F.A. Browne, L.J. Parent, K. Julian, B. Bozdogan, P.C. Appelbaum, J. Chaitram, L.M. Weigel, J. Jernigan, L.K. McDougal, F.C. Tenover, and S.K. Fridkin Vancomycin-resistant Staphylococcus aureus in the absence of vancomycin exposure Clin. Infect. Dis. 38 2004 1049 1055