Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay

Journal of Clinical Microbiology

Volumn 41, Issue 6, 2003, Pages 2483-2486

  Palladino, Silvano ^a   Kay, Ian D ^a   Flexman, James P ^a   Boehm, Ingrid ^a   Costa, Anna Maria G ^a   Lambert, Erica J ^a   Christiansen, Keryn J ^a  

^a ROYAL PERTH HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PRIMER DNA; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CLINICAL ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC ACCURACY; DNA EXTRACTION; ENRICHMENT CULTURE; ENTEROCOCCUS; ENTEROCOCCUS FAECIUM; FECES CULTURE; GENE IDENTIFICATION; HUMAN; MOLECULAR PROBE; MULTIPLEX REAL TIME POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TECHNIQUE;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECIUM; POSIBACTERIA;

EID: 0038180716     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.6.2483-2486.2003     Document Type: Article

References (18)

1. Chen, Y., S. A. Marshall, P. L. Winokur, S. L. Coffman, W. W. Wilke, P. R. Murray, C. A. Spiegel, M. A. Pfaller, G. V. Doern, and R. N. Jones. 1998. Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci. J. Clin. Microbiol. 36:2996-3001.
2. Coombs, G. W., I. D. Kay, R. A. Steven, J. W. Pearman, D. Bertolatti, and W. B. Grubb. 1999. Should genotypic testing be done on all phenotypically vancomycin-resistant enterococci detected in hospitals? J. Clin. Microbiol. 37:1229-1230.
3. Dutka-Malen, S., S. Evers, and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33:24-27.
4. Emori, T. G., and R. P. Gaynes. 1993. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin. Microbiol. Rev. 6:428-442.
5. Facklam, R., and D. F. Sahm. 1995. Enterococcus, p. 308-314. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C.
6. Fluit, A. D. C., R. V. Maarten, and F. J. Schmitz. 2001. Molecular detection of antimicrobial resistance. Clin. Microbiol. Rev. 14:836-871.
7. Hospital Infection Control Practices Advisory Committee. 1995. Recommendations for preventing the spread of vancomycin resistance. Morb. Mortal. Wkly. Rep. 44(RR-12):1-13.
8. Ieven, M., E. Vercauteren, P. Descheemaeker, F. van Laer, and H. Goossens. 1999. Comparison of direct plating and broth enrichment culture for the detection of intestinal colonization by glycopeptide-resistant enterococci among hospitalized patients. J. Clin. Microbiol. 37:1436-1440.
9. Jayaratne, P., and C. Rutherford. 1999. Detection of clinically relevant genotypes of vancomycin-resistant enterococci in nosocomial surveillance specimens by PCR. J. Clin. Microbiol. 37:2090-2092.
10. Jones, R. N., S. A. Marshall, M. A. Pfaller, W. W. Wilke, R. J. Hollis, M. E. Erwin, M. B. Edmond, and R. P. Wenzel. 1997. Nosocomial enterococcal blood stream infections in the SCOPE program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. Diagn. Microbiol. Infect. Dis. 29:95-102.
11. Lu, J. J., C. L. Perng, T. S. Chiueh, S. Y. Lee, C. H. Chen, F. Y. Chang, C. C. Wang, and W. M. Chi. 2001. Detection and typing of vancomycin-resistance genes of enterococci from clinical and nosocomial surveillance specimens by multiplex PCR. Epidemiol. Infect. 126:357-363.
12. National Committee for Clinical Laboratory Standards. 1995. Molecular diagnostic methods for infectious diseases; approved guideline. MM3-A. National Committee for Clinical Laboratory Standards, Wayne, Pa.
13. National Committee for Clinical Laboratory Standards. 2001. Performance standards for antimicrobial disk susceptibility tests, 7th ed. Approved standard. M2-A7 and M100-S12. National Committee for Clinical Laboratory Standards, Wayne, Pa.
14. Palladino, S., I. D. Kay, A. G. Costa, E. J. Lambert, and J. P. Flexman. 2002. Real-time PCR for the rapid detection of vana and vanB genes. Diagn. Microbiol. Infect. Dis. 45:81-84.
15. Petrich, A. K., K. E. Luinistra, D. Groves, M. A. Chernesky, and J. B. Mahony. 1999. Direct detection of vana and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR. Mol. Cell. Probes 13:275-281.
16. Roger, M., M. C. Faucher, P. Forest, P. St-Antoine, and F. Coutlée. 1999. Evaluation of a VanA-specific PCR assay for detection of vancomycin-resistant Enterococcusfaecium during a hospital outbreak. J. Clin. Microbiol. 37:3348-3349.
17. Sahm, D. F., L. Free, C. Smith, M. Eveland, and L. M. Mundy. 1997. Rapid characterization schemes for surveillance isolates of vancomycin-resistant enterococci. J. Clin. Microbiol. 35:2026-2030.
18. Satake, S., N. Clark, D. Rimland, F. S. Nolte, and F. C. Tenover. 1997. Detection of vancomycin-resistant enterococci in fecal samples by PCR. J. Clin. Microbiol. 35:2325-2330.