Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: A promising approach for the production of gypenosides as an alternative of ginseng saponins

Biotechnology Letters

Volumn 27, Issue 16, 2005, Pages 1165-1169

  Chang, Chen Kai ^a   Chang, Ku Shang ^b   Lin, Yi Chen ^a   Liu, Sin Yie ^c   Chen, Chien Yuan ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b YUANPEI UNIVERSITY   (Taiwan)

^c TAIWAN AGRICULTURAL RESEARCH INSTITUTE   (Taiwan)

Author keywords

Gynostemma pentaphyllum; Gypenosides; Hairy root cultures; Jiaogulan

Indexed keywords

BACTERIA; BIOMASS; GENES;

GYNOSTEMMA PENTAPHYLLUM; GYPENOSIDES; HAIRY ROOT CULTURES; JIAOGULAN;

PLANTS (BOTANY);

GYPENOSIDE; PLANT EXTRACT; RECOMBINANT PROTEIN; SAPONIN;

ARTICLE; BIOSYNTHESIS; GENETIC TRANSFECTION; GENETICS; GINSENG; GYNOSTEMMA; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLANT ROOT; TRANSGENIC PLANT;

CLONING, MOLECULAR; GYNOSTEMMA; PANAX; PLANT EXTRACTS; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SAPONINS; TRANSFECTION;

AGROBACTERIUM RHIZOGENES; GYNOSTEMMA; GYNOSTEMMA PENTAPHYLLUM; PANAX GINSENG;

EID: 24744460023     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-005-8653-7     Document Type: Article

References (15)

1. CO Akalezi S Liu QS Li JT Yu JJ Zhong 1999 Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng Process Biochem 34 639 642
2. R Bhadra JV Shanks 1997 Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots of Catharanthus roseus Biotechnol. Bioeng 55 527 534
3. HM Fei KF Mei X Shen YM Ye ZP Lin LH Peng 1993 Transformation of Gynostemma pentaphyllum by Agrobacterium rhizogenes and saponin production in hairy root cultures Acta Bot. Sin 35 626 631
4. OL Gamborg RA Miller K Ojima 1968 Nutrient requirements of suspension cultures of soybean root cells Exp. Cell Res 50 151 158
5. TSC Li G Mazza AC Cottrell L Gao 1996 Ginsenosides in roots and leaves of American ginseng J. Agric. Food Chem 44 717 720
6. SB Liu R Lin ZH Hu 2005 Histochemical localization of ginsenosides in Gynostemma pentaphyllum and the content changes of total gypenosides Acta Biol. Exp. Sin 38 54 60
7. T Murashige F Skoog 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473 497
8. Å Norberg NK Hoa E Liepinsh DV Phan ND Thuan H Jörnvall R Sillard C-G Östenson 2004 A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum J. Biol. Chem 279 41361 41367
9. GF Payne V Bringi C Prince ML Shuler 1992 Plant Cell and Tissue Culture in Liquid Systems Hanser Publishing New York
10. N Sevón K-M Oksman-Caldentey 2002 Agrobacterium rhizogenes-mediated transformation: Root cultures as a source of alkaloids Planta Med 68 859 868
11. MA Subroto PM Doran 1994 Production of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid Plant Cell Tiss. Org. Cult 38 93 102
12. D Thomson R Henry 1995 Single-step protocol for preparation of plant tissue for analysis by PCR BioTechniques 19 394 400
13. G Vervliet M Holsters H Teuchy M van Montagu J Schell 1975 Characterization of different plaque forming and defective temperate phages in Agrobacterium strains J. Gen. Virol 26 33 48
14. PDG Wilson MG Hilton RJ Robins MJC Rhodes 1987 Fermentation studies of transformed root cultures GW, Moody PB Baker Bioreactors and Biotransformation Elsevier Press London 38 51
15. F Yin LH Hu RX Pan 2004 Novel dammarane-type glycosides from Gynostemma pentaphyllum Chem. Pharm. Bull 52 1440 1444