Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots of Catharanthus roseus

Biotechnology and Bioengineering

Volumn 55, Issue 3, 1997, Pages 527-534

  Bhadra, Rajiv ^a,b   Shanks, Jacqueline V ^a  

^a Rice University   (United States)

^b RICE UNIVERSITY   (United States)

Author keywords

Catharanthus roseus; Growth association; Hairy roots; Indole alkaladdies; Nutrients; Organic acids

Indexed keywords

GROWTH KINETICS; ORGANIC ACIDS; PH EFFECTS;

CATHARANTHUS ROSEUS; HAIRY ROOTS; INDOLE ALKALOIDS; NUTRIENT UPTAKE;

PLANT CELL CULTURE;

CARBOXYLIC ACID; INDOLE ALKALOID;

ARTICLE; BIOMASS; CALORIC INTAKE; CULTURE MEDIUM; NUTRIENT; PLANT GROWTH; PLANT ROOT; TISSUE CULTURE;

CATHARANTHUS ROSEUS;

EID: 0031554711     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970805)55:3<527::AID-BIT9>3.0.CO;2-E     Document Type: Article

References (42)

1. Aerts, R. J., Gisi, D., De Carolis, E., De Luca, V., Baumann, T. 1994. Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings. Plant J 5: 635-643.
2. Bhadra, R., Shanks, J. V. 1995. Statistical design of the effect of inoculum conditions on growth of hairy roots of Catharanthus roseus. Biotechnol. Tech. 9: 681-686.
3. Bhadra, R., Vani, S. N., Shanks, J. V. 1993. Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol. Bioeng. 41: 581-592.
4. Ciau-Uitz, R., Miranda-Ham, M. L., Coello-Coello, J., Chi, B., Pacheco, L. M., Loyola-Vargas, V. M. 1994. Indole alkaloid production by transformed and non-transformed root cultures of Catharanthus roseus. In Vitro Cell. Dev. Biol. 30P: 84-88.
5. Constabel, F., Gaudet-La Prairie, P., Kurz, W. G. W., Kutney, J. P. 1982. Alkaloid production in Catharanthus roseus cell cultures. XII. Biosynthetic capacity of callus from original explants and regenerated shoots. Plant Cell Rep. 1: 139-142.
6. De Luca, V. 1990. Enzymology of indole alkaloid biosynthesis. Methods Plant Biochem. 9: 346-368.
7. Fowler, M. W. 1982. Substrate utilization by plant-cell cultures. J. Chem. Tech. Biotechnol. 32: 338-346.
8. Gamborg, O. L., Miller, R. A., Ojima, K. 1968. Nutrient requirements of suspension cultures of soyabean root cells. Exp. Cell Res. 50: 151-158.
9. Ganapathi, B., Kargi, F. 1990. Recent advances in indole alkaloid production by Catharanthus roseus (periwinkle). J. Exper. Bot. 41: 259-267.
10. Hamill, J. D., Parr, A. J., Rhodes, M. J. C., Robins, R. J., Walton, N. J. 1987. New routes to plant secondary products. Biotechnology 5: 800-804.
11. Hirata, K., Yamanaka, A., Kurano, N., Miyamoto, K., Miura, Y. 1987. Production of indole alkaloids in multiple shoot culture of Catharanthus roseus (L). G. Don. Agric. Biol. Chem. 51: 1311-1317.
12. Islas, I., Loyola-Vargas, V. M., Miranda-Ham, M. d. L. 1994. Tryptophan decarboxylase activity in transformed roots from Catharanthus roseus and its relationship to tryptamine, ajmalicine, and catharanthine accumulation during the culture cycle. In Vitro Cell. Dev. Biol. 30P: 81-83.
13. Jung, K. H., Kwak, S. S., Kim, S. W., Lee, H., Choi, C. Y., Liu, J. R. 1992. Improvement of the catharanthine productivity in hairy root cultures of Catharanthus roseus by using monosaccharides as a carbon source. Biotechnol. Lett. 14: 695-700.
14. Kurz, W. G. W., Chatson, K. B., Constabel, F., Kutney, J. P., Choi, L. S. L., Kolodziejczyk, P., Sleigh, S. K., Stuart, K. L., Worth, B. R. 1980. Alkaloid production in Catharanthus roseus cell cultures. IV. Characterization of the 953 cell line. Helv. Chim. Acta. 63: 1891-1895.
15. Kutney, J. P., Choi, L. S. L., Kolodziejczyk, P., Sleigh, S. K., Stuart, K. L., Worth, B. R., Kurz, W. G. W., Constabel, F. 1980. Alkaloid production in Catharanthus roseus cell cultures: Isolation and characterization of alkaloids from one cell line. Phytochemistry 19: 2589-2595.
16. Lee, C. W. T., Shuler, M. L. 1991. Different shake flask closures alter gas phase composition and ajmalicine production in Catharanthus roseus cell suspensions. Biotechnol. Tech. 5: 173-178.
17. Meijer, A. H., Verpoorte, R., Hoge, J. H. C. 1993. Regulation of enzymes and genes involved in terpenoid indole alkaloid biosynthesis in Catharanthus roseus. J. Plant Res. Special Issue 3: 145-164.
18. Menegus, F., Cattaruzza, L., Chersi, A., Fronza, G. 1989. Differences in the anaerobic lactate-succinate production and in the changes of cell sap pH for plants with high and low resistance to anoxia. Plant Physiol. 90: 29-32.
19. Merillon, J. M., Rideau, M., Cheneiux, J. C. 1984. Influence of sucrose on levels of ajmalicine, serpentine and tryptamine in Catharanthus roseus cells in vitro. Planta Med. 50: 497-502.
20. Mersey, B. G., Cutler, A. J. 1986. Differential distribution of specific indole alkaloids in leaves of Catharanthus roseus. Can. J. Bot. 64: 1039-1045.
21. Moreno, P. R. H., van der Heijden, R., Verpoorte, R. 1995. Cell and tissue cultures of Catharanthus roseus: A literature survey. II. Updating from 1988 to 1993. Plant Cell Tiss. Organ Cult. 42: 1-25.
22. Morris, P. 1986. Regulation of product synthesis in cell cultures of Catharanthus roseus. II. Comparison of production media. Planta Med. 52: 121-126.
23. Morris, P., Fowler, M. 1980. Sucrose utilization by cell suspension cultures of Catharanthus roseus G. Don. Biochem. Soc. Trans. 8: 630-631.
24. Parr, A. J., Peerless, A. C. J., Hamill, J. D., Walton, N. J., Robins, R. J., Rhodes, M. J. C. 1988. Alkaloid production by transformed root cultures of Catharanthus roseus. Plant Cell Rep. 7: 309-312.
25. Payne, G. F., Bringi, V., Prince, C., Shuler, M. L. 1992. Plant cell and tissue culture in liquid systems. Hanser. Munich.
26. Roberts, J. K. M., Callis, J., Wemmer, D., Walbot, V., Jardetzky, O. 1984. Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia. Proc. Natl. Acad. Sci. USA 81: 3379-3383.
27. Robins, R. J., Walton, N. J., Hamill, J. D., Parr, A. J., Rhodes, M. J. C. 1991. Strategies for the genetic manipulation of alkaloid-producing pathways in plants. Planta Med. 57: S27-S35.
28. + dependent nitrate uptake by Catharanthus roseus cells: electrophysiological effects. Bioelectr. Bioener. 25: 213-223.
29. Ryu, D. D. Y., Lee, S. O., Romani, R. J. 1990. Determination of growth rate of plant cell cultures: Comparative studies. Biotechnol. Bioeng. 35:305-311.
30. Sakano, K. 1990. Proton/phosphate stoichiomctry in uptake of inorganic phosphate by cultured cells of Catharanthus roseus (L.) G. Don. Plant Physiol. 93: 479-483.
31. Schlatmann, J. E., Moreno, P. R. H., Selles, M., Vinke, J. L., ten Hoopen, H. G. J., Verpoorte, R., Heijnen, J. J. 1995. Two-stage batch process for the production of ajmalicine by Catharanthus roseus: The link between growth and production stage. Biotechnol. Bioeng. 47: 53-59.
32. Scragg, A. H., Ashton, S., York, A., Bond, P., Stepan-Sarkissian, G., Grey, D. 1990. Growth of Catharanthus roseus suspensions for maximum biomass and alkaloid accumulation. Enz. Microb. Technol. 12: 292-298.
33. Shanks, J. V., Bhadra, R. 1997. Characteristics of selected hairy root lines of Catharanthus roseus. In: P. M. Doran (ed.), Hairy roots. Harwood Academic, Reading, MA.
34. Sim, S. J., Chang, H. N., Liu, J.R., Jung, K. H. 1994. Production and secretion of indole alkaloids in hairy root cultures of Catharanthus ro7 seus: Effects of in situ adsorption, fungal elicitation and permeabilization. J. Ferm. Bio. 78: 229-234.
35. Stockigt, J., Soll, H. J. 1980. Indole alkaloids from cell suspension cultures of Catharanthus roseus and C. ovalis. Planta Med. 40: 22-30.
36. Toivonen, L., Balsevich, J., Kurz, W. G. W. 1989. Indole alkaloid production by hairy root cultures of Catharanthus roseus. Plant Cell Tiss. Organ Cult. 18: 79-83.
37. Toivonen, L., Ojala, M., Kauppinen, V. 1990. Indole alkaloid production by hairy root cultures of Catharanthus roseus: Growth kinetics and fermentation. Biotechnol. Lett. 12: 519-524.
38. Toivonen, L., Ojala, M., Kauppinen, V. 1991. Studies on the optimization of growth and alkaloid production by hairy root cultures of Catharanthus roseus. Biotechnol. Bioeng. 37: 673-680.
39. Ullrich, W. R. 1992. Transport of nitrate and ammonium through plant membranes, pp. 121-137. In: K. Mengel and D. J. Pilbeam (eds.), Nitrogen metabolism in plants. Clarendon, Oxford.
40. Vani, S. N. 1996. Bioreactor design for scaleup of Catharanthus roseus hairy root cultures for production of indole alkaloids. Ph.D. Thesis, Rice University, Houston, TX.
41. Vasquez-Flota, F., Moreno-Valanzuela, O., Miranda-Ham, M. L., Coello-Coello, J., Loyola-Vargas, V. M. 1994. Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures. Plant Cell Tiss. Organ Cult. 38: 273-279.
42. Yu, S., Doran, P. M. 1994. Oxygen requirements and mass transfer in hairy-root cultures. Biotechnol. Bioeng. 44: 880-887.