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Volumn 44, Issue 34, 2005, Pages 5480-5483

Label-free detection of protein-protein interactions on biochips

Author keywords

Bioinformatics; Immobilization; Mass spectrometry; Monolayers; Proteins

Indexed keywords

ENZYME IMMOBILIZATION; GOLD; MASS SPECTROMETRY; MICROPROCESSOR CHIPS; MONOLAYERS; SELF ASSEMBLY;

EID: 24644467294     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200501363     Document Type: Article
Times cited : (62)

References (35)
  • 13
    • 24644467094 scopus 로고    scopus 로고
    • note
    • 1H NMR spectra. Synthetic schemes and experimental details are provided in the Supporting Information.
  • 14
    • 4544316763 scopus 로고    scopus 로고
    • The peak at mlz 693.6 was derived from a symmetric disulfide of tri(ethylene glycol)-terminated alkanethiolates. Sodium adducts of disulfides are the major species, which are observed in MALDI spectra of SAMs of alkanethiolates; see: J. Su, M. Mrksich, Angew. Chem. 2002, 114, 4909;
    • (2002) Angew. Chem. , vol.114 , pp. 4909
    • Su, J.1    Mrksich, M.2
  • 17
    • 24644478430 scopus 로고    scopus 로고
    • note
    • GST fusions with src homology domain 3 (GST-SH3) and with green fluorescent protein (GST-GFP) were assayed with similar results.
  • 22
    • 24644435306 scopus 로고    scopus 로고
    • note
    • β.
  • 26
    • 24644462102 scopus 로고    scopus 로고
    • note
    • In the MALDI MS data of GST-SRC, two other peaks at mlz 27.3 kDa and m/z 32.6 kDa were observed. We believe that these peaks represent GST and a degradation product of GST-SRC that still contains GST which were co-purified on a glutathione column.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.