Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates

Angewandte Chemie - International Edition

Volumn 41, Issue 24, 2002, Pages 4715-4718

  Su, Jing ^a   Mrksich, Milan ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Biochips; Carbohydrates; Glycosylation; Mass spectrometry; Monolayers

Indexed keywords

CARBOHYDRATES; ENZYMES; FLUORESCENCE; MASS SPECTROMETRY; MONOLAYERS; PROTEINS; RADIOACTIVITY; SUBSTRATES;

BIOCHIPS;

SELF ASSEMBLY;

CARBOHYDRATE; LIGAND; PEPTIDE; PROTEIN;

ANALYTIC METHOD; ARTICLE; CARBOHYDRATE ANALYSIS; CHEMICAL LABELING; CHEMISTRY; CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; ISOTOPE LABELING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHODOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN INTERACTION;

CARBOHYDRATE CONFORMATION; CARBOHYDRATES; LIGANDS; PEPTIDES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY;

EID: 0037122032     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200290026     Document Type: Article

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20. When reporting the ratio of alkanethiolates present in a mixed SAM, we give the ratio of alkanethiols in the solutions used for the formation of the monolayer.
21. The majority of the SAMs in this work gave mainly sodium adducts of the molecular ions. Throughout the text we provide empirical formulas and the expected monoisotopic masses for each observed disulfide.
22. We found that application of a matrix was necessary for the observation of molecular ions from the monolayers, but we have not optimized the formulation of the matrix for these applications.
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