Strain improvement for cephalosporin production by Acremonium chrysogenum using geneticin as a suitable transformation marker

FEMS Microbiology Letters

Volumn 235, Issue 1, 2004, Pages 43-49

  Rodríguez Sáiz, Marta ^a   Lembo, Marianna ^b   Bertetti, Luca ^b   Muraca, Roberto ^b   Velasco, Javier ^a   Malcangi, Antonella ^b   De La Fuente, Juan Luis ^a   Barredo, José Luis ^a  

^a Avenida de Antibioóticos 59 61   (Spain)

^b R+D Biology   (Italy)

Author keywords

Acremonium; Cephalosporin; Geneticin; Improvement; Marker; Resistance; Transformation

Indexed keywords

ACYLTRANSFERASE; ANTIBIOTIC G 418; DEACETYLCEPHALOSPORIN C; PHLEOMYCIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM TRANSFORMATION; CEPHALOSPORIUM ACREMONIUM; DRUG MANUFACTURE; ENZYME ACTIVITY; GENE CONSTRUCT; GENETIC TRANSCRIPTION; IMMUNOBLOTTING; MARKER GENE; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ACREMONIUM; ANTI-BACTERIAL AGENTS; CEPHALOSPORINS; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; GENETIC MARKERS; GENTAMICINS; TRANSFORMATION, GENETIC;

ACREMONIUM CHRYSOGENUM;

EID: 2442705556     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.04.010     Document Type: Article

References (35)

1. Díez B., Mellado E., Rodríguez M., Fouces R., Barredo J.L. Recombinant microorganisms for industrial production of antibiotics. Biotechnol. Bioeng. 55:1997;216-226
2. Gutiérrez S., Díez B., Montenegro E., Martín J.F. Characterization of the Cephalosporium acremonium pcbAB gene encoding α-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin- biosynthetic genes and evidence of multiple functional domains. J. Bacteriol. 173:1991;2354-2365
3. Samson S.M., Belagaje R., Blankenship D.T., Chapman J.L., Perry D., Skatrud P.L., Vankfrank R.M., Abraham E.P., Baldwin J.E., Queener S.W., Ingolia T.D. Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium. Nature. 318:1985;191-194
4. Samson S.M., Dotzlaf J.E., Slisz M.L., Becker G.W., van Frank R.M., Veal L.E., Yeh W., Miller J.R., Queener S.W., Ingolia T.D. Cloning and expression of the fungal expandase/hydroxylase gene involved in cephalosporin biosynthesis. Biotechnology. 5:1987;1207-1214
5. Gutiérrez S., Velasco J., Fernández F.J., Martín J.F. The cefG gene of Cephalosporium acremonium is linked to the cefEF and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase. J. Bacteriol. 174:1992;3056-3064
6. Ullán R.V., Casqueiro J., Bañuelos O., Fernández F.J., Gutiérrez S., Martín J.F. A novel epimerization system in fungal secondary metabolism involved in the conversion of isopenicillin N into penicillin N in Acremonium chrysogenum. J. Biol. Chem. 277:2002;46216-46225
7. Gutiérrez S., Díez B., Álvarez E., Barredo J.L., Martín J.F. Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants. Mol. Gen. Genet. 225:1991;56-64
8. Velasco J., Adrio J., Moreno M.A., Díez B., Soler G., Barredo J.L. Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum. Nat. Biotechnol. 18:2000;857-861
9. Skatrud P.L., Queener S.W., Carr L.G., Fisher D.L. Efficient integrative transformation of C. acremonium. Curr. Genet. 12:1987;337-348
10. Skatrud P.L., Tietz A.J., Ingolia T.D., Cantwell C.A., Fisher D.L., Chapman J.L., Queener S.W. Use of recombinant DNA to improve production of cephalosporin C by Cephalosporium acremonium. Biotechnology. 7:1989;477-485
11. Nowak C., Kuck U. Development of an homologous transformation system from Acremonium chrysogenum based on the beta-tubulin gene. Curr. Genet. 25:1994;34-40
12. Carramolino L., Lozano M., Pérez-Aranda A., Rubio V., Sánchez F. Transformation of Penicillium chrysogenum to sulfonamide resistance. Gene. 77:1989;31-38
13. Bull J.H., Smith D.J., Turner G. Transformation of Penicillium chrysogenum with a dominant selectable marker. Curr. Genet. 13:1988;377-382
14. Ward J.M., Wilkinson B., Turner G. Transformation of Aspergillus nidulans with a cloned oligomycin-resistant ATP synthase subunit 9 gene. Mol. Gen. Genet. 202:1986;265-270
15. Tilburn J., Scazzocchio C., Taylor G.G., Zabicky-Zissman J.H., Lockington R.A., Davies R.W. Transformation by integration in Aspergillus nidulans. Gene. 26:1983;205-221
16. Rambosek J., Leach J. Recombinant DNA in filamentous fungi: progress and prospects. CRC Crit. Rev. Biotechnol. 6:1987;357-393
17. Cantoral J.M., Díez B., Barredo J.L., Álvarez E., Martín J.F. High frequency transformation of Penicillium chrysogenum. Biotechnology. 5:1987;494-497
18. Beri R.J., Turner G. Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker. Curr. Genet. 11:1987;639-641
19. Sánchez F., Lozano M., Rubio V., Peñalva M.A. Transformation in Penicillium chrysogenum. Gene. 51:1987;97-102
20. Whitehead M.P., Unkles S.E., Ramsden M., Campbell E.I., Gurr S.J., Spence D., van den Hondel C., Contreras R., Kinghorn J.R. Transformation of a nitrate reductase deficient mutant of Penicillium chrysogenum with the corresponding Aspergillus niger and A. nidulans niaD genes. Mol. Gen. Genet. 216:1989;408-411
21. Yelton M.M., Hamer J.E., Timberlake W.E. Transformation of Aspergillus nidulans using a trpC plasmid. Proc. Natl. Acad. Sci. USA. 81:1984;1470-1474
22. Wernars K., Goosen T., Wennekes L.M.J., Visser J., Bos C.J., van den Broeck H.W.J., van Gorcom R.F.M., van den Hondel C.A.M.J.J., Pouwels P.H. Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene. Curr. Genet. 9:1985;361-368
23. Unkles S.E., Campbell E.I., Carrez D., Grieve C., Contreras R., Fiers W., van den Hondel C.A.M.J.J., Kinghorn J.R. Transformation of Aspergillus niger with the homologous nitrate reductase gene. Gene. 78:1989;157-166
24. Peñalva M.A., Touriño A., Patiño C., Sánchez F., Fernández-Sousa J.M., Rubio V. Studies of transformation of Cephalosporium acremonium. Timberlake W.E. Molecular Genetics of Filamentous Fungi. 1985;59-68 Alan R Liss Inc. New York
25. Muraca, R., Velasco, J., Cerrato, M., Díez, B., Barredo, J.L. and Bernasconi, E. (1998) Improvement of cephalosporin C production by A. chrysogenum. In: Proceedings of the Eighth International Symposium on the Genetics of Industrial Microorganisms (GIM98), p. 63. Jerusalem, Israel
26. Sambrook K.J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. 1990;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
27. Punt P.J., Dingemanse M.A., Kuyvenhoven A., Soede R.D., Pouwels P.H., van den Hondel C.A. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene. 93:1990;101-109
28. Díez B., Mellado E., Rodríguez M., Bernasconi E., Barredo J.L. The NADP-dependent glutamate dehydrogenase gene from Penicillium chrysogenum and the construction of expression vectors for filamentous fungi. Appl. Microbiol. Biotechnol. 52:1999;196-207
29. Chen P.Y., Wang C.K., Soong S.C., To K.Y. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol. Breed. 11:2003;287-293
30. Specht C.A., DiRusso C.C., Novotny C.P., Ullrich R.C. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal. Biochem. 119:1982;158-163
31. Díez B., Velasco J., Marcos A.T., Rodríguez M., De La Fuente J.L., Barredo J.L. The gene encoding. γ -actin from the cephalosporin producer Acremonium chrysogenum Appl. Microbiol. Biotechnol. 54:2000;786-791
32. Ausubel F.M., Brent R., Kingston R.E., Moore D., Smith J.A., Seidman J.G., Struhl K. Current Protocols in Molecular Biology. 1987;John Wiley & Sons, New York
33. Ramos F.R., López-Nieto M.J., Martín J.F. Isopenicillin N synthetase of Penicillium chrysogenum, an enzyme that converts delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to isopenicillin N. Antimicrob. Agents Chemother. 27:1985;380-387
34. Gutiérrez S., Velasco J., Marcos A.T., Fernandez F.J., Fierro F., Barredo J.L., Díez B., Martín J.F. Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum. Appl. Microbiol. Biotechnol. 48:1997;606-614
35. Hua J., Meyer J.D., Lodge J.K. Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans. Clin. Diagn. Lab. Immunol. 7:2000;125-128