The N-degron approach to create temperature-sensitive mutants in Schizosaccharomyces pombe

Methods

Volumn 33, Issue 3, 2004, Pages 206-212

  Rajagopalan, Srividya ^a   Liling, Zheng ^a   Liu, Jianhua ^a   Balasubramanian, Mohan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

bir1; N Degron; N end rule; Schizosaccharomyces pombe; ts mutants

Indexed keywords

ARGININE; DIHYDROFOLATE REDUCTASE; FUNGAL PROTEIN; PROTEIN BIR1; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

ALLELE; AMINO TERMINAL DEGRON METHOD; AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; CHROMOSOME SEGREGATION; CONTROLLED STUDY; FUNGAL GENE; FUNGAL STRAIN; GENE DELETION; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; TEMPERATURE DEPENDENCE; TEMPERATURE SENSITIVE MUTANT; YEAST;

MUTATION; PEPTIDE FRAGMENTS; SCHIZOSACCHAROMYCES; TEMPERATURE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 2442575658     PISSN: 10462023     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymeth.2003.11.015     Document Type: Article

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