Expression of the Apx toxins of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae and its induction of immune response in mice

Biotechnology and Bioprocess Engineering

Volumn 10, Issue 4, 2005, Pages 362-366

  Park, Seung Moon ^a   Choi, Eun Jin ^a   Kwon, Tae Ho ^a   Jang, Yong Suk ^a   Yoo, Han Sang ^b   Choi, Woo Bong ^c   Park, Bong Kyun ^b   Kim, Dae Hyuk ^a  

^a Chonbuk National University   (South Korea)

^b Seoul National University   (South Korea)

^c Dong Eui University   (South Korea)

Author keywords

Actinobacillus pleuropneumoniae; ApxIA; ApxIIA; Saccharomyces cerevisiae; Vaccines

Indexed keywords

ALCOHOL DEHYDROGENASE; BACTERIAL TOXIN; BACTERIAL VACCINE; FREUND ADJUVANT; IMMUNOGLOBULIN G ANTIBODY;

ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ANTIBODY TITER; ANTIGEN SPECIFICITY; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HYBRID; IMMUNE RESPONSE; IMMUNITY; IMMUNIZATION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PNEUMONIA; PROMOTER REGION; PROVOCATION TEST; SACCHAROMYCES CEREVISIAE; SURVIVAL RATE; SWINE; WESTERN BLOTTING;

ACTINOBACILLUS PLEUROPNEUMONIAE; MYCOPLASMA; SACCHAROMYCES CEREVISIAE; SUS SCROFA;

EID: 24344500774     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931856     Document Type: Article

References (28)

1. Sebunya, T. N. and J. R. Saunders (1983) Haemophilus pleuropneumoniae infection in swine J. Am. Vet. Med. Assoc. 182: 1331-1337.
2. Bosse, J. T., H. Janson, B. J. Sheehan, A. J. Beddek, A. N. Rycroft, J. S. Kroll, and P. R. Langford (2000) Actinobacillus pleuropneumoniae: Pathobiology and pathogenesis of infection. Microb. Infect. 4: 225-235.
3. Frey, J. (1995) Virulence in Actinobacillus pleuropneumoniae and RTX toxins. Trends Microbiol. 3: 257-261.
4. Dubreuil, J. D., M. Jacques, K. R. Mittal, and M. Gottschalk (2000) Actionbacillus pleuropneumoniae surface polysaccharides: Their role in diagnosis and immunogenicity. Anim. Health. Res. Rev. 1: 73-93.
5. Anderson, C., A. A. Potter, and G. F. Gerlach (1999) Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7. Infect. Immun. 59: 4110-4116.
6. Fuller, T. E., S. Martin, J. F. Teel, G. R. Alaniz, M. J. Kennedy, and D. E. Lowery (2000) Identification of Actinobacillus pleuropneumoniae virulence genes using signature-tagged mutagenesis in a swine infection model. Microb. Pathog. 29: 39-51.
7. Prideaux, C. T., C. Lenghaus, J. Krywult, and A. L. M. Hodgson (1999) Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. Infect. Immun. 67: 1962-1966.
8. Tascon, R. I., J. A. Vanquez-Boland, C. B. Gutierrez-Martin, I. Rodriguez-Barbosa, and E. F. Rodriguez-Ferri (1994) The RTX haemolysin ApxI and ApxII are major virulence factors of the swine pathogen Actinobacillus pleuropneumoniae: Evidence from mutational analysis. Mol. Microbiol. 14: 207-216.
9. Bosse, J. T., H. Janson, B. J. Sheehan, A. J. Beddek, A. N. Rycroft, J. S. Kroll, and P. R. Langford (2002) Actinobacillus pleuropneumoniae: Pathobiology and pathogenesis of infection. Micro. Infect. 4: 225-235.
10. Seah, J. N., J. Frey, and J. Kwang (2002) The N-terminal domain of RTX toxin ApxI of Actinobacillus pleuropneumoniae elicits protective immunity in mice. Infect. Immun. 70: 6464-6467.
11. Fenwick, B. and S. Henry (1994) Porcine pleuropneumonia. J. Am. Vet. Med. Assoc. 204: 1334-1340.
12. Tascon, R. I., J. A. Vazquez-Boland, C. B. Gutierrez-Martin, J. I. Rodriguez-Barbosa, and E. F. Rodriguez-Ferri (1996) Virulence factors of the swine pathogen Actinobacillus pleuropneumoniae. Microbiologia 12: 171-184.
13. Satran, P., K. Nedbalcova, and Z. Kucerova (2003) Comparison of protection efficacy of toxoid and whole-cell vaccines against porcine pleuropneumonia caused by endotracheal infection with Actinobacillus pleuropneumoniae. Acta. Veterinaria. Brno. 72: 213-219.
14. Rombaut, B. and J. P. M. Jore (1997) Immunogenic non-infectious polio subviral particles synthesized in Saccaromyces cerveseae. J. Gen. Virol. 78: 1829-1832.
15. Surgrue, R. J., J. Fu, J. Howe, and Y. C. Chan (1997) Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. J. Gen. Virol. 78: 1861-1866.
16. Brake, A. J., J. P. Merryweather, D. G. Coit, U. A. Heberlein, T. P. Masiary, G. T. Mullenback, M. S. Urdea, P. Valenzuela, and P. J. Barr (1984) Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 81: 4642-4646.
17. Ramisetti, S., H. A. Kang, S. K. Rhee, and C. H. Kim (2003) Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding. Biotechnol. Bioprecess Eng. 8: 183-186.
18. Park, S. M. and D. H. Kim (2004) Transformation of a filamentous fungus Cryphonectria parasitica using Agrobacterium tumefaciens. Biotechnol. Bioprecess Eng. 9: 217-222.
19. Schreuder, M. P., C. Deen, W. J. A. Boersma, P. H. Pouwels, and F. M. Klis (1996) Yeast expressing hepatitis B virus antigen determinants on its surface: implications for a possible oral vaccine. Vaccine 14: 383-388.
20. Stubbs, A. C., K. S. Martin, C. Coeshott, S. V. Skaates, D. R. Kuritzkes, D. Bellgrau, A. Franzusoff, R. C. Duke, and C. C. Wilson (2001) Whole recombinant yeast vaccine activates dendritic cells and elicits protective cell-mediated immunity. Nat. Med. 7: 625-629.
21. Shin, S. J., Y. W. Cho, and H. S. Yoo (2003) Cloning, sequencing and expression of apxIA, IIA, IIIA of Actinobacillus pleuropneumoniae isolated in Korea. Kor. J. Vet. Res. 43: 247-253.
22. Park, S. M., A. Y. Mo, Y. S. Jang, J. H. Lee, M. S. Yang, and D. H. Kim (2004) Expression of a functional human Tumor Necrosis Factor (hTNF)-α in Yeast Saccharomyces cerevisiae. Biotechnol. Bioprecess Eng. 9: 292-296.
23. Ito, H., Y. Fukuda, K. Murata, and A. Kimura (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
24. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
25. Towbin, H., T. Staehelin, and J. Gorden (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
26. Schreuder, M. P., C. Deen, W. J. A. Boersma, P. H. Pouwels, and F. M. Klis (1996) Yeast expressing hepatitis B virus antigen determinants on its surface: Implications for a possible oral vaccine. Vaccine 14: 383-388.
27. Bathurst, I. C. (1994) Protein expression in yeast as an approach to production of recombinant malaria antigens. Am. J. Trop. Med. Hyg. 50: 11-19.
28. Schreuder, M. P., A. T. A. Mooren, H. Y. Toschka, T. C. Verrips, and F. M. Klis (1996) Immobilizing proteins on the surface of yeast cells. Trends. Biotechnol. 14: 115-120.