Molecular and functional expression of voltage-operated calcium channels during osteogenic differentiation of human mesenchymal stem cells

Journal of Bone and Mineral Research

Volumn 20, Issue 9, 2005, Pages 1637-1646

  Zahanich, Ihor ^a   Graf, Eva M ^a   Heubach, Jürgen F ^a   Hempel, Ute ^a   Boxberger, Sabine ^a   Ravens, Ursula ^a,b  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Bone marrow; L type Ca2+ channel; Mesenchymal stem cells; Nifedipine; Patch clamp technique

Indexed keywords

ALKALINE PHOSPHATASE; ASCORBIC ACID; CALCIUM; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM CHANNEL L TYPE; CALCIUM ION; DEXAMETHASONE; DIHYDROPYRIDINE; GLYCEROL 2 PHOSPHATE; NIFEDIPINE; OSTEOCLAST DIFFERENTIATION FACTOR; OSTEOPONTIN; OSTEOPROTEGERIN; PHOSPHATE; VOLTAGE GATED CALCIUM CHANNEL;

ADULT; ARTICLE; BONE DEVELOPMENT; BONE MINERALIZATION; CALCIUM CURRENT; CELL DIFFERENTIATION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MESENCHYMAL STEM CELL; NORMAL HUMAN; OSTEOBLAST; PATCH CLAMP; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STATISTICAL SIGNIFICANCE;

ADULT; ALKALINE PHOSPHATASE; ANIMALS; ASCORBIC ACID; BONE MARROW; BONE MARROW CELLS; CALCIUM; CALCIUM CHANNELS, L-TYPE; CALCIUM CHANNELS, T-TYPE; CARRIER PROTEINS; CELL DIFFERENTIATION; CELL LINE; CULTURE MEDIA; DEXAMETHASONE; DNA PRIMERS; FLOW CYTOMETRY; GLYCEROPHOSPHATES; GLYCOPROTEINS; HUMANS; MEMBRANE GLYCOPROTEINS; MESENCHYMAL STEM CELLS; MICROSCOPY, PHASE-CONTRAST; MIDDLE AGED; NERVE TISSUE PROTEINS; NIFEDIPINE; OSTEOBLASTS; OSTEOPONTIN; OSTEOPROTEGERIN; PATCH-CLAMP TECHNIQUES; PHOSPHATES; POLYMERASE CHAIN REACTION; RANK LIGAND; RATS; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, TUMOR NECROSIS FACTOR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SIALOGLYCOPROTEINS;

EID: 24144502034     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/JBMR.050521     Document Type: Article

References (34)

1. Friedenstein AJ, Chailakhyan RK, Gerasimov UV 1987 Bone marrow osteogenic stem cells: In vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 20:263-272.
2. Caplan AI 1991 Mesenchymal stem cells. J Orthop Res 9:641-650.
3. Haynesworth SE, Goshima J, Goldberg VM, Caplan AI 1992 Characterization of cells with osteogenic potential from human bone marrow. Bone 13:81-88.
4. Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J, Moorman M, Simonetti D, Craig S, Marschak D 1999 Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.
5. Muraglia A, Cancedda R, Quatro R 2000 Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J Cell Sci 113:1161-1166.
6. Minguell JJ, Erices A, Gonget P 2001 Mesenchymal stem cells. Exp Biol Med 226:507-520.
7. Malaval L, Modrowski D, Gupta AK, Modrowski D, Gupta AK, Aubin JE 1994 Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures. J Cell Physiol 158:555-572.
8. 2+ in normal human osteoblast-like cells: Aspects of their regulation. Biochem Biophys Res Commun 263:206-212.
9. Jorgensen NR, Teilmann SC, Henriksen Z, Civitelli R, Sorensen OH, Steinberg TH 2003 Activation of L-type calcium channels is required for gap junction-mediated intercellular calcium signaling in osteoblastic cells. J Biol Chem 278:4082-4086.
10. Guggino SE, Lajeunesse D, Wagner JA, Snyder SH 1989 Bone remodeling signalled by a dihydropyridine- and phenylalkylamine-sensitive calcium channel. Proc Natl Acad Sci USA 86:2957-2960.
11. 2+ channel inhibition in a rat osteoblast-like cell line, UMR 106, by a new dihydropyridine derivative, S11568. Eur J Pharmacol 220:11-17.
12. 1 subunit of voltage-gated calcium channels in human osteoblast-like cell lines and in normal human osteoblasts. Calcif Tissue Int 66:145-150.
13. 1D isoform by parathyroid hormone. Proc Natl Acad Sci USA 92:10914-10918.
14. 2+ channels in osteocytic (MLO-Y4) cells. Biochem Biophys Res Commun 282:536-542.
15. 2+ signalling pathways in human mesenchymal stem cells. Cell Calcium 32:165-174.
16. Heubach JF, Graf EM, Leutheuser J, Bock M, Balana B, Zahanich I, Christ T, Boxberger S, Wettwer E, Ravens U 2004 Electrophysiological properties of human mesenchymal stem cells. J Physiol 554:659-672.
17. Majumdar MK, Thiede MA, Mosca JD, Moorman M, Gerson SL 1998 Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol 176:57-66.
18. Chomczynski P, Sacchi N 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
19. Senger M, Flores T, Glatting K-H, Ernst P, Hotz-Wagenblatt A, Suhai S 1998 W2H: WWW interface to the GCG sequence analysis package. Bioinformatics 14:452-457.
20. 2+ channel in persistent atrial fibrillation in humans. Z Kardiol 89:IV/23-IV/29.
21. Schultz JH, Volk T, Ehmke H 2001 Heterogeneity of Kv2.1 mRNA expression and delayed rectifier current in single isolated myocytes from rat left ventricle. Circ Res 88:483-490.
22. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ 1981 Improved patch clamp technique for high resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85-100.
23. 2+ currents coexist in guinea pig ventricular myocytes and are both blocked by mibefradil. Circ Res 86:628-635.
24. Fabiato A, Fabiato F 1979 Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 75:463-505.
25. Nishiya Y, Sugimoto S 2001 Effects of various antihypertensive drugs on the function of osteoblast. Biol Pharm Bull 24:628-633.
26. Maeda H, Wada N, Nakamuta H, Akamine A 2004 Human peripheral granulation tissue contains osteogenic cells. Cell Tissue Res 315:203-208.
27. 1G subunits. Pflugers Arch 438:553-560.
28. 2+ channels mediate mechanically induced bone formation in vivo. J Bone Miner Res 17:1795-1800.
29. Yang Y, Magnay JL, Cooling L, El Haj AJ 2002 Development of a 'mechano-active' scaffold for tissue engineering. Biomaterials 23:2119-2126.
30. Nishiya Y, Kosaka N, Uchii M, Sugimoto S 2002 A potent 1,4-dihydropyridine L-type calcium channel blocker, benidipine, promotes osteoblast differentiation. Calcif Tissue Int 70:30-39.
31. Ritchie CK, Maercklein PB, Fitzpatrick LA 1994 Direct effect of calcium channel antagonists on osteoclast function: Alterations in bone resorption and intracellular calcium concentrations. Endocrinology 135:996-1003.
32. 2+ channel in cultured bone marrow stromal cells by dexamethasone. Calcif Tissue Int 54:125-132.
33. Conget P, Minguell J 1999 Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 181:67-73.
34. Colter D, Class R, DiGirolamo C, Prockop D 2000 Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci USA 97:3213-3218.