A collagen IV matrix is required for guinea pig gastric epithelial cell monolayers to provide an optimal model of the stomach surface for biopharmaceutical screening

Journal of Biomolecular Screening

Volumn 10, Issue 5, 2005, Pages 495-507

  Kavvada, Klairi M ^a   Murray, James G ^b   Moore, Vanessa A ^b   Coombes, Alan G A ^a   Hanson, Peter J ^a  

^a ASTON UNIVERSITY   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

Author keywords

Gastric; Mucous cell; Nonsteroidal anti inflammatory drug; Stomach; Transwells

Indexed keywords

5 (4 CHLOROPHENYL) 1 (4 METHOXYPHENYL) 3 TRIFLUOROMETHYL 1H PYRAZOLE; 5 (4 FLUOROPHENYL) 1 [(4 METHYLSULFONYL)PHENYL] 3 TRIFLUOROMETHYLPYRAZOLE; AMPHOTERICIN B; ANTHRANILIC ACID; CHENODEOXYCHOLIC ACID; COLLAGEN TYPE 1; COLLAGEN TYPE 4; DICLOFENAC; FLUORESCEIN; GLUCOSAMINE; GLYCOPROTEIN; IBUPROFEN; INDOLEACETIC ACID; INDOMETACIN; KETOPROFEN; MANNITOL; MEFENAMIC ACID; NAPROXEN; NICOTINIC ACID; NIFLUMIC ACID; NONSTEROID ANTIINFLAMMATORY AGENT; OXICAM DERIVATIVE; PHENYLACETIC ACID; PIROXICAM; POLYCARBONATE; PROPIONIC ACID; PROPRANOLOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; SUPEROXIDE; UNINDEXED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DRUG SCREENING; ELECTRIC RESISTANCE; EPITHELIUM CELL; FILTER; GUINEA PIG; NONHUMAN; PERIODIC ACID SCHIFF STAIN; PRIORITY JOURNAL; STOMACH; STOMACH EPITHELIUM;

ANIMALS; ANTI-INFLAMMATORY AGENTS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; BODY WEIGHT; CELL CULTURE TECHNIQUES; COLLAGEN; COLLAGEN TYPE I; COLLAGEN TYPE IV; DICLOFENAC; EPITHELIAL CELLS; GASTRIC MUCOSA; GLUCOSAMINE; GLYCOPROTEINS; GUINEA PIGS; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MUCINS; NADPH OXIDASE; NIFLUMIC ACID; PERMEABILITY; POLYCARBOXYLATE CEMENT; STOMACH; SUPEROXIDES; TECHNOLOGY, PHARMACEUTICAL; TIME FACTORS;

CAVIA PORCELLUS;

EID: 24144448970     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057105276035     Document Type: Article

References (32)

1. Artursson P, Palm K, Luthman K: Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv Drug Del Rev 2001;46:27-43.
2. Tavelin S, Milovic V, Ocklind G, Olsson S, Artursson P: A conditionally immortalised epithelial cell line for studies of intestinal drug transport. J Pharm Exp Ther 1999;290:1212-1221.
3. Versantvoort CHM, Ondrewater RCA, Duizer E, Van De Sandt JJM, Gilde AJ, Groten JP: Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: comparison of active and passive transport with the human colon carcinoma Caco-2 cell line. Env Tox Pharm 2002;11:335-344.
4. Ito S: Functional gastric morphology. In Johnson LR (ed): Physiology of the Gastrointestinal Tract, 2nd ed. New York: Raven Press, 1987:817-848.
5. Chen MC, Chang A, BuhlT, Tanner M, Soll AH: Apical acidification induces paracellular injury in canine gastric mucosal monolayers. Am J Physiol 1994;267:G1012-G1020.
6. Rutten M, Rattner D, Silen W: Transepithelial transport of guinea-pig gastric mucous cell monolayers. Am J Physiol 1985;249:C503-C513.
7. Yanaka A, Muto H, Fukutomi H, Ito S, Silen W: Role of nitric oxide in restitution of injured guinea-pig gastric mucosa in vitro. Am J Physiol 1995;268:G933-G942.
8. Allen A, Flemstrom G, Garner A, Kivilaakso E: Gastroduodenal mucosal protection. Physiol Rev 1993;73:823-857.
9. Teshima S, Rokutan K, Nikawa T, Kishi K: Guinea-pig gastric mucosal cells produce abundant superoxide anion through an NADPH oxidase-like system. Gastroenterology 1998;115:1186-1196.
10. Rokutan K, Kutsumi H, Nakamura K, Teshima S, Nikawa T: Human gastric pit cells express phagocyte NADPH oxidase components and secrete superoxide anion in vivo. Gastroenterology 1999;116:G1291.
11. Hawkey CJ: Nonsteroidal anti-inflammatory drug gastropathy. Gastroenterology 2000;119:521-535.
12. Ashton M, Hanson, PJ: Disparate effects of non-steroidal anti-inflammatory drugs on apoptosis in cultured guinea-pig gastric mucous cells: inhibition by diclofenac. Br J Pharmacol 2002;135:407-416.
13. Beinborn M, Giebel J, Linck M, Cetin Y, Schwenk M, Sewing K-F: Isolation, identification and quantitative evaluation of specific cell types from the mammalian gastric mucosa. Cell Tissue Res 1993;274:229-240.
14. Brown JF, Keates AC, Whittle BJR, Hanson PJ: Nitric oxide generators and cyclic GMP stimulate mucus secretion by rat gastric mucosal cells. Am J Physiol 1993;265:G418-G422.
15. Takahashi S, Okabe S: The cytoprotective effect of leminoprazole on indomethacin-induced damage to rabbit gastric mucosal cells. J Pharmacol Exp Ther 1996;279:975-982.
16. McCormack K, Brune K: The amphiprotic character of azapropazone and its relevance to the gastric mucosa. Arch Toxicol 1990;64:1-6.
17. Warner TD, Giuliano F, Vojnovic I, Bukasa A, Mitchell JA, Vane JR: Nonsteroid drug selectivities for cyclooxygenase-1 rather than cyclooxygenase-2 are associated with human gastrointestinal toxicity: a full in vitro analysis. Proc Natl Acad Sci U S A 1999;96:7563-7568.
18. Ehrlich K, Sicking C, Respondek M, Peskar BM: Interaction of cyclooxygenase isoenzymes, nitric oxide and afferent neurons in gastric mucosal defense in rats. J Pharmacol Exp Ther 2004;308:277-283.
19. Ota MD, Tsukahara H, Terano A, Hata Y, Hiraishi H, Mutoh H, Sugimoto T: Protective effect of tauroursodeoxycholate against chenodeoxycholate-induced damage to cultured rabbit gastric cells. Dig Dis Sci 1991;36:409-416.
20. Tegeder I, Pfeilschifter J, Geisslinger G: Cyclooxygenase-independent actions of cyclooxygenase inhibitors. FASEB J 2001;15:2057-2072.
21. Sakamoto C, Matsuda K, Nakano O, Konda Y, Matozaki T, Nishisaki H, Kasuga M: EGF stimulates both cyclooxygenase activity and cell proliferation of cultured guinea-pig gastric mucous cells. J Gastroenterol 1994;29(suppl. VII):73-76.
22. Hollande F, Blanc EM, Bali JP, Whitehead RH, Pelegrin A, Baldwin GS, Choquet A: HGF regulates tight junctions in new nontumorigenic gastric epithelial cell line. Am J Physiol 2001;280:G910-G921.
23. Drubin DG, Nelson WJ: Origins of cell polarity. Cell 1996;84:335-344.
24. Kreis T, Vale R: Guidebook to the Extracellular Matrix, Anchor and Adhesion Proteins. 2nd ed. Oxford, UK: Oxford University Press, 1999.
25. Bravo SA, Nielsen CU, Amstrup J, Frokjaer S, Brodin B: In-depth evaluation of Gly-Sar transport parameters as a function of culture time in the Caco-2 cell model. Eur J Pharm Sci 2004;21:77-86.
26. Takao T, Shimamoto C, Hirata I, Oshiba S: The effect of acid secretagogues on mucin synthesis using a primary monolayer culture of the guinea-pig gastric mucous cells. Gastroenterol Jap 1993;28:638-646.
27. Chanock S, Benna JE, Smith RM, Babior BM: The respiratory burst oxidase. J Biol Chem 1994;269:24519-24522.
28. Spencer GE, Spragg CF, Stables R, Hirst BH: Transmucosal electrical resistance in rabbit isolated gastric mucosa during exposure to acid. J Physiol 1992;449:169-181.
29. Shorrock CJ, Prescott RJ, Rees WDW: The effect of indomethacin on gastroduodenal morphology and mucosal pH gradient in the healthy human stomach. Gastroenterology 1990;99:334-339.
30. Garcia Rodriguez LA, Jick H: Risk of upper gastrointestinal bleeding and perforation associated with individual non-steroidal anti-inflammatory drugs. Lancet 1994;343:769-772.
31. Wallace JL, McKnight W, Reuter BK, Vergnolle N: NSAID-induced gastric damage in rats: requirement for inhibition of both cyclooxygenase 1 and 2. Gastroenterology 2000;119:706-714.
32. Suzuki H, Yanaka A, Shibahara T, Matsui H, Nakahara A, Tanaka N, Muto H, Momoi T, Uchiyama Y: Ammonia-induced apoptosis is accelerated at higher pH in gastric surface mucous cells. Am J Physiol 2002;283:G986-G995.