Partitioning and purification of extracellular p-1,3-1,4-glucanase in aqueous two-phase systems

Journal of Zhejiang University: Science

Volumn 6 B, Issue 8, 2005, Pages 825-831

  He, Guo Qing ^a   Zhang, Xiu Yan ^a   Tang, Xing Jun ^a   Chen, Qi He ^a   Ruan, Hui ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

1,3 1,4 glucanase; Aqueous two phase system; Partition

Indexed keywords

FERMENTATION; MOLECULAR WEIGHT; PH; POLYETHYLENE GLYCOLS; PURIFICATION; SODIUM CHLORIDE;

AQUEOUS TWO PHASE SYSTEM; CRUDE ENZYME PREPARATION; ENZYMES PARTITION; FERMENTATION BROTH; GLUCANASE; MGSO4; NEUTRAL PROTEASES; PARTITION BEHAVIORS;

ENZYMES;

ENDO 1,3(4) BETA GLUCANASE; WATER;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; EVALUATION; EXTRACELLULAR FLUID; FRACTIONATION; ISOLATION AND PURIFICATION; METHODOLOGY; PHASE TRANSITION;

BACILLUS SUBTILIS; CHEMICAL FRACTIONATION; ENDO-1,3(4)-BETA-GLUCANASE; EXTRACELLULAR FLUID; PHASE TRANSITION; WATER;

EID: 24044458243     PISSN: 10093095     EISSN: None     Source Type: Journal    
DOI: 10.1631/jzus.2005.B0825     Document Type: Article

References (17)

1. Edney, M.J., Marchylo, B.A., Macgregor, A.W., 1991. Structure of total barley-glucan. J. Inst. Brew., 97: 39-44.
2. Godfrey, T., Reichely, J., 1983. Industrial Enzymology. McMillan, London, p.466.
3. Han, J.H., Lee, C.H., 1997. Effects of salts and poly (ethylene glycol)-palmitate on the partitioning of proteins and Bacillus subtilis neutral protease in aqueous two-phase systems. Colloids and Surfaces B: Biointerfaces, 9: 109-116.
4. Huddleston, J.G., Veide, A.K., 1991. The molecular basis of partitioning in aqueous two-phase systems. TIBTECH, 9: 381-388.
5. Jain, A., Johri, B.N., 1999. Partitioning of an extracellular xylanase produced by a thermophilic fungus Melanocarpus albomyces IIS-68 in an aqueous two-phase system. Bioresource Technology, 67: 205-207.
6. Jiao, Q.C., Liu, X., Chen, Y.Z., 1998. Studies on the separation and extraction of α-amylase and proteinase from fermented liquor of α-amylase by aqueous two-phase partitioning. Chem. J. Chinese Universities, 3: 391 (in Chinese).
7. Johansson, G., Reczey, K., 1998. Concentration and purification of β-glucosidase from Aspergillus niger by using aqueous two-phase partitioning. Journal of Chromatography B, 711: 161-172.
8. Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426-427.
9. Pan, I.H., Li, Y.K., 2001. Rapid process for purification of an extracellular β-xylosidase by aqueous two-phase extraction. Journal of Chromatography B, 754: 179-184.
10. Planas, A., 2000. Bacterial 1,3-1,4-β-glucanases: Structure, function and protein engineering. Biochimica et Biophysica Acta, 1543: 361-382.
11. Schmidt, A.S., Ventom, A.M., Asenjo, J.A., 1994. Partitioning and purification of α-amylase in aqueous two-phase systems. Enzyme Microb. Technol., 16: 131-142.
12. Stone, B.A., Clarke, A.E., 1992. Chemistry and Biology of 1,3-β-Glucans. La Trobe University Press, Bundoora, Australia.
13. Syu, M.J., Chen, Y.H., 1997. A study on the α-amylase fermentation performed by Bacillus amyloliquefaciens. Chemical Engineering Journal, 65: 237-247.
14. Xiao, J.X., Bao, Y.X., Fang, L., 2001. Phase behavior and protein partitioning in aqueous two-phase systems formed by mixtures of polymer and cationic-anionic surfactants. Chem. J. Chinese Universities, 22(1): 112-114.
15. White, W.B., Bird, H.R., Sunde, M.L., Marlett, J.A., 1983. Poult. Sci., 62: 853-862.
16. Woodward, J.R., Phillips, D.R., Fincher, G.B., 1983. Water soluble (1-3), (1-4)-β-D-glucans from barley (Hordeum vulgare) endosperm. I. Physicochemical properties. Carbohydr. Polym., 3: 143-156.
17. Zhang, L.X., Zhang, T.F., Li, L.Y., 1997. Method and Technology of Biochemistry Experiments. Higher Education Press, Beijing (in Chinese).