Bimolecular fluorescence complementation analysis of cytochrome P450 2C2, 2E1, and NADPH-cytochrome P450 reductase molecular interactions in living cells

Drug Metabolism and Disposition

Volumn 33, Issue 9, 2005, Pages 1382-1390

  Ozalp, Cengiz ^a   Szczesna Skorupa, Elzbieta ^a   Kemper, Byron ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME; CYTOCHROME P450; CYTOCHROME P450 2C1; CYTOCHROME P450 2C2; CYTOCHROME P450 2E1; LAURIC ACID; PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIMOLECULAR FLUORESCENCE COMPLEMENTATION; CELL MEMBRANE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATION ANALYSIS; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SEQUENCE ANALYSIS;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; CYTOCHROME P-450 CYP2E1; CYTOCHROME P-450 ENZYME SYSTEM; FLOW CYTOMETRY; FLUORESCENCE; HUMANS; LAURIC ACIDS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; NADPH-FERRIHEMOPROTEIN REDUCTASE; PROTEIN INTERACTION MAPPING; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 23944462892     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.105.005538     Document Type: Article

References (40)

1. Alston K, Robinson RC, Park SS, Gelboin HV, and Friedman FK (1991) Interactions among cytochromes P-450 in the endoplasmic reticulum. Detection of chemically cross-linked complexes with monoclonal antibodies. J Biol Chem 266:735-739.
2. Amet Y, Berthou F, Baird S, Dreano Y, Bail JP, and Menez JF (1995) Validation of the (omega-1)-hydroxylation of lauric acid as an in vitro substrate probe for human liver CYP2E1. Biochem Pharmacol 50:1775-1782.
3. Atmakuri K, Ding Z, and Christie PJ (2003) VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens. Mol Microbiol 49:1699-1713.
4. Backes WL and Kelley RW (2003) Organization of multiple cytochrome P450s with NADPH-cytochrome P450 reductase in membranes. Pharmacol Ther 98:221-233.
5. Bridges A, Gruenke L, Chang YT, Vakser IA, Loew G, and Waskell L (1998) Identification of the binding site on cytochrome P450 2B4 for cytochrome b5 and cytochrome P450 reductase. J Biol Chem 273:17036-17049.
6. Causey KM, Eyer CS, and Backes WL (1990) Dual role of phospholipid in the reconstitution of cytochrome P-450 LM2-dependent activities. Mol Pharmacol 38:134-142.
7. Cawley GF, Zhang S, Kelley RW, and Backes WL (2001) Evidence supporting the interaction of CYP2B4 and CYP1A2 in microsomal preparations. Drug Metab Dispos 29:1529-1534.
8. Dean WL and Gray RD (1982) Relationship between state of aggregation and catalytic activity of cytochrome P-450LM2 and NADPH-cytochrome P-450 reductase. J Biol Chem 257:14679-14685.
9. Eidne KA, Kroeger KM, and Hanyaloglu AC (2002) Applications of novel resonance energy transfer techniques to study dynamic hormone receptor interactions in living cells. Trends Endocrinol Metab 13:415-421.
10. Estabrook RW, Franklin MR, Cohen B, Shigamatzu A, and Hildebrandt AG (1971) Biochemical and genetic factors influencing drug metabolism. Influence of hepatic microsomal mixed function oxidation reactions on cellular metabolic control. Metabolism 20:187-199.
11. French JS, Guengerich FP, and Coon MJ (1980) Interactions of cytochrome P-450, NADPH-cytochrome P-450 reductase, phospholipid and substrate in the reconstituted liver microsomal enzyme system. J Biol Chem 255:4112-4119.
12. Gonzalez FJ (1990) Molecular genetics of the P-450 superfamily. Pharmacol Ther 45:1-38.
13. Gut J, Richter C, Cherry RJ, Winterhalter KH, and Kawato S (1982) Rotation of cytochrome P-450. II. Specific interactions of cytochrome P-450 with NADPH-cytochrome P-450 reductase in phospholipid vesicles. J Biol Chem 257:7030-7036.
14. Gut J, Richter C, Cherry RJ, Winterhalter KH and Kawato S (1983) Rotation of cytochrome P-450. Complex formation of cytochrome P-450 with NADPH-cytochrome P-450 reductase in liposomes demonstrated by combining protein rotation with antibody-induced cross-linking. J Biol Chem 258:8588-8594.
15. Hu CD, Chinenov Y, and Kerppola TK (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9:789-798.
16. Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, and Berlot CH (2004) Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. J Biol Chem 279:30279-30286.
17. Imai Y (1988) Characterization of rabbit liver cytochrome P-450 (laurate (ω-1) hydroxylase) synthesized in transformed yeast cells. J Biochem (Tokyo) 103:143-148.
18. Kanaeva IP, Dedinskii IR, Skotselyas ED, Krainev AG, Guleva IV, Sevryukova IF, Koen YM, Kuznetsova GP, Bachmanova GI, and Archakov AI (1992a) Comparative study of monomeric reconstituted and membrane microsomal monooxygenase systems of the rabbit liver. I. Properties of NADPH-cytochrome P450 reductase and cytochrome P450 LM2 (2B4) monomers. Arch Biochem Biophys 298:395-402.
19. Kanaeva IP, Nikityuk OV, Davydov DR, Dedinskii IR, Koen YM, Kuznetsova GP, Skotselyas ED, Bachmanova GI, and Archakov AI (1992b) Comparative study of monomeric reconstituted and membrane microsomal monooxygenase systems of the rabbit liver. II. Kinetic parameters of reductase and monooxygenase reactions. Arch Biochem Biophys 298:403-412.
20. Kanno T, Kanno Y, Siegel RM, Jang MK, Lenardo MJ, and Ozato K (2004) Selective recognition of acetylated histones by bromodomain proteins visualized in living cells. Mol Cell 13:33-43.
21. Kawato S, Gut J, Cherry RJ, Winterhalter KH, and Richter C (1982) Rotation of cytochrome P-450. I. Investigations of protein-protein interactions of cytochrome P-450 in phospholipid vesicles and liver microsomes. J Biol Chem 257:7023-7029.
22. Levin MH, Haggie PM, Vetrivel L, and Verkman AS (2001) Diffusion in the endoplasmic reticulum of an aquaporin-2 mutant causing human nephrogenic diabetes insipidus. J Biol Chem 276:21331-21336.
23. Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, Hamilton AD, and Regan L (2005) Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J Am Chem Soc 127:146-157.
24. Miwa GT and Lu AY (1984) The association of cytochrome P-450 and NADPH-cytochrome P-450 reductase in phospholipid membranes. Arch Biochem Biophys 234:161-166.
25. Myasoedova KN and Berndt P (1990) Cytochrome P-450LM2 oligomers in proteoliposomes. FEBS Lett 275:235-238.
26. Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM, and Nebert DW (2004) Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants. Pharmacogenetics 14:1-18.
27. Omata Y, Dai R, Smith SV, Robinson RC, and Friedman FK (2000) Synthetic peptide mimics of a predicted topographical interaction surface: the cytochrome P450 2B1 recognition domain for NADPH-cytochrome P450 reductase. J Protein Chem 19:23-32.
28. Peterson JA, Ebel RE, O'Keeffe DH, Matsubara T, and Estabrook RW (1976) Temperature dependence of cytochrome P-450 reduction. A model for NADPH-cytochrome P-450 reductase:cytochrome P-450 interaction. J Biol Chem 251:4010-4016.
29. Remy I and Michnick SW (2004) A cDNA library functional screening strategy based on fluorescent protein complementation assays to identify novel components of signaling pathways. Methods 32:381-388.
30. Schwarz D, Pirrwitz J, Meyer HW, Coon MJ, and Ruckpaul K (1990) Membrane topology of microsomal cytochrome P-450: saturation transfer EPR and freeze-fracture electron microscopy studies. Biochem Biophys Res Commun 171:175-181.
31. Schwarz D, Pirrwitz J, and Ruckpaul K (1982) Rotational diffusion of cytochrome P-450 in the microsomal membrane-Evidence for a clusterlike organization from saturation transfer electron paramagnetic resonance spectroscopy. Arch Biochem Biophys 216:322-328.
32. Scott EE, White MA, He YA, Johnson EF, Stout CD, and Halpert JR (2004) Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl) imidazole at 1.9-A resolution: insight into the range of P450 conformations and the coordination of redox partner binding. J Biol Chem 279:27294-27301.
33. Shimada T, Mernaugh RL, and Guengerich FP (2005) Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase and cytochrome b(5) enzymes. Arch Biochem Biophys 435:207-216.
34. Szczesna-Skorupa E, Chen C, and Kemper B (2000) Cytochromes P450 2C1/2 and P450 2E1 are retained in the endoplasmic reticulum membrane by different mechanisms. Arch Biochem Biophys 374:128-136.
35. Szczesna-Skorupa E, Chen C, Rogers S, and Kemper B (1998) Mobility of cytochrome P450 in the endoplasmic reticulum membrane. Proc Natl Acad Sci USA 95:14793-14798.
36. Szczesna-Skorupa E and Kemper B (2001) The juxtamembrane sequence of cytochrome P-450 2C1 contains an endoplasmic reticulum retention signal. J Biol Chem 276:45009-45014.
37. Szczesna-Skorupa E, Mallah B, and Kemper B (2003) Fluorescence resonance energy transfer analysis of cytochromes P450 2C2 and 2E1 molecular interactions in living cells. J Biol Chem 278:31269-31276.
38. Voznesensky AI and Schenkman JB (1994) Quantitative analyses of electrostatic interactions between NADPH-cytochrome P450 reductase and cytochrome P450 enzymes. J Biol Chem 269:15724-15732.
39. Watanabe J, Asaka Y, Fujimoto S, and Kanamura S (1993) Densities of NADPH-ferrihemoprotein reductase and cytochrome P-450 molecules in the endoplasmic reticulum membrane of rat hepatocytes. J Histochem Cytochem 41:43-49.
40. Wu ES and Yang CS (1984) Lateral diffusion of cytochrome P-450 in phospholipid bilayers. Biochemistry 23:28-33.