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Volumn 15, Issue 19, 2005, Pages 4275-4278

Novel inhibitors of the hepatitis C virus NS3 proteinase

Author keywords

Hepatitis; Proteinase

Indexed keywords

4 METHYL 1 (PHENYLMETHYL) 2,6 PYRIDINEDIONE; DIMER; PROTEINASE INHIBITOR; SILICA GEL; UNCLASSIFIED DRUG; VIRUS ENZYME;

EID: 23944459090     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.06.079     Document Type: Article
Times cited : (3)

References (26)
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    • Hepatitis C Treatment
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    • D. Bresters Hepatitis C Treatment H.W. Reesink Hepatitis C Virus 1994 Karger Basel 121
    • (1994) Hepatitis C Virus , pp. 121
    • Bresters, D.1
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    • 0000361013 scopus 로고
    • Hepatitis C Virus
    • 3rd ed. B.N. Fields D.M. Knipe D.M. Howley Raven Press New York
    • M. Houghton Hepatitis C Virus 3rd ed. B.N. Fields D.M. Knipe D.M. Howley Virology Vol. 1 1995 Raven Press New York 1035 1058
    • (1995) Virology , vol.1 , pp. 1035-1058
    • Houghton, M.1
  • 7
    • 0001424128 scopus 로고
    • Flaviviridae: The Viruses and Their Replication
    • 3rd ed. B.N. Fields D.M. Knipe D.M. Howley Raven Press New York
    • C.M. Rice Flaviviridae: The Viruses and Their Replication 3rd ed. B.N. Fields D.M. Knipe D.M. Howley Virology Vol. 1 1995 Raven Press New York 931 960
    • (1995) Virology , vol.1 , pp. 931-960
    • Rice, C.M.1
  • 10
    • 33645398623 scopus 로고    scopus 로고
    • note
    • 35S- labeled translated substrate in a 20 μl volume containing 10 mM Tris, pH 7.5, 120 mM NaCl, 5 mM DTT, 0.5% EDTA, 0.1% Tween 20, and 12% glycerol followed by incubation at 30°C for 30 min. Inhibitors were added to assay mixtures prior to incubation. In-house standards of HCV protease inhibitor molecules served as positive controls. Cleavage reactions were terminated by addition of an equal volume of 2x Laemmli sample buffer and boiling for 3 min. Cleavage products were analyzed by SDS/15% PAGE gel electrophoresis and autoradiography.
  • 21
    • 33645409906 scopus 로고    scopus 로고
    • note
    • 6 requires 457.1400.
  • 24
    • 33645404206 scopus 로고    scopus 로고
    • note
    • 2 and in-house standards of HCV protease inhibitor molecules served as positive controls. After stopping the reaction, cleavage of the Cys-Ser bond was detected by the addition of streptavidin-coated SPA beads (Amersham), which can bind to the biotin-labeled peptide. Cleavage is indicated by reduction in signal caused by separation of the radiolabeled fragment from the bound biotinylated fragment (measured in CPM) which is proportional to proteolytic activity.
  • 25
    • 33645409526 scopus 로고    scopus 로고
    • note
    • 3 requires 230.0817.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.