An improved method for determination of gene copy numbers in transgenic mice by serial dilution curves obtained by real-time quantitative PCR assay

Journal of Biochemical and Biophysical Methods

Volumn 64, Issue 2, 2005, Pages 83-98

  Mitrečić, Dinko ^a   Huzak, Miljenko ^b   Ćurlin, Marija ^a   Gajović, Srećko ^a  

^a UNIVERSITY OF ZAGREB   (Croatia)

^b UNIVERSITY OF ZAGREB   (Croatia)

Author keywords

Gene copy number; Real time quantitative PCR; TaqMan; Transgenic mice; Zygosity

Indexed keywords

ACCURACY; ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION ANALYSIS; DILUTION CURVE; GENE DELETION; GENE NUMBER; MOUSE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STANDARD; STATISTICAL PARAMETERS; TRANSGENE; TRANSGENIC MOUSE; ZYGOSITY;

ANALYSIS OF VARIANCE; ANIMALS; CROSSES, GENETIC; DNA; GENE DELETION; GENE DOSAGE; GENETIC TECHNIQUES; GENOTYPE; HETEROZYGOTE; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MODELS, STATISTICAL; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSGENES;

ANIMALIA; MUS MUSCULUS;

EID: 23844470961     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2005.05.006     Document Type: Article

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