A new gene trap construct enriching for insertion events near the 5′ end of genes

Transgenic Research

Volumn 9, Issue 6, 2000, Pages 395-404

  Thomas, Tim ^a   Voss, Anne K ^a   Chowdhury, Kamal ^a   Gruss, Peter ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Gene trap; Vectors

Indexed keywords

BETA GALACTOSIDASE; ELECTROPORATION; ENZYME ACTIVITY; GENE INSERTION SEQUENCE; GENE INSERTION; KANAMYCIN KINASE; PLASMID VECTOR;

ANIMALS; BASE SEQUENCE; BETA-GALACTOSIDASE; CHIMERA; ELECTROPORATION; FEMALE; GENES, REPORTER; GENETIC TECHNIQUES; GENETIC VECTORS; KANAMYCIN KINASE; MALE; MICE; MICE, INBRED STRAINS; MOLECULAR SEQUENCE DATA; STEM CELLS;

EID: 0034500589     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026595111913     Document Type: Article

References (28)

1. Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence
2. Ontogenic expression of the erythroid-type glucose transporter (Glut 1) in the telencephalon of the mouse: Correlation to the tightening of the blood-brain barrier
3. Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases
4. Evidence for the stochastic integration of gene trap vectors into the mouse germline
5. Regulation and expression of a growth arrest-specific gene (gas5) during growth, differentiation, and development
6. Developmental regulation of the distribution of rat brain insulin-insensitive (Glut 1) glucose transporter
7. Structure and expression pattern of the murine Hox-3.2 gene
8. Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice
9. Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer
10. A survey on intron and exon lengths
11. RNA splicing
12. Molecular basis of β-Galactosidase alpha complementation
13. Production of completely ES cell-derived fetuses
14. 3T3-L1 adipocyte glucose transporter (HepG2 class): Sequence and regulation of protein and mRNA expression by insulin, differentiation, and glucose starvation
15. DNA sequencing with chain-termination inhibitors
16. Capturing genes encoding membrane and secreted proteins important for development
17. The mouse Enhancer trap locus 1 (Etl-1): A novel mammalian gene related to Drosophila and yeast transcriptional regulator genes
18. Molecular characterization of the murine argininosuccinate synthetase locus
19. Gene trap capture of a novel mouse gene, jumoniji, required for neural tube formation
20. Differential screening of a PCR generated mouse embryo cDNA library: Glucose transporters are differentially expressed in the early postimplantation mouse embryo
21. An alpha-E-catenin gene trap mutation defines its function in preimplantation development
22. Germ line chimeras from female ES cells
23. Efficiency assessment of the gene trap approach
24. A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure
25. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells
26. A study of the urea-produced subunits of β-galactosidase