Apoptin nuclear accumulation is modulated by a CRM1-recognized nuclear export signal that is active in normal but not in tumor cells

Cancer Research

Volumn 65, Issue 16, 2005, Pages 7059-7064

  Poon, Ivan K H ^a   Oro, Cristina ^a   Dias, Manisha M ^a   Zhang, Jingpu ^c   Jans, David A ^a,b  

^a MONASH UNIVERSITY   (Australia)

^b Australian Government Australian Research Council   (Australia)

^c INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTIN; EXPORTIN 1; LEUCINE; THREONINE;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL SPECIFICITY; HUMAN; HUMAN CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; NUCLEOCYTOPLASMIC TRANSPORT; PHOSPHORYLATION; PRIORITY JOURNAL; PROMYELOCYTIC LEUKEMIA; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION; TUMOR CELL;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID SEQUENCE; ANIMALS; CAPSID PROTEINS; CELL LINE, TUMOR; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; COS CELLS; GREEN FLUORESCENT PROTEINS; HUMANS; KARYOPHERINS; LEUCINE; LEUKEMIA, PROMYELOCYTIC, ACUTE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR LOCALIZATION SIGNALS; OSTEOSARCOMA; PEPTIDE FRAGMENTS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; THREONINE; TRANSFECTION;

EID: 23844445433     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: 10.1158/0008-5472.CAN-05-1370     Document Type: Article

References (20)

1. Oro C, Jans DA. The tumor specific proapoptolic factor apoptin (VP3) from chicken anaemia virus. Curr Drug Targets 2004;5:179-90.
2. Danen-van Oorschot AAAM, Fischer DF, Grimbergen JM, et al. Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells. Proc Natl Acad Sci U S A 1997;94;5843-7.
3. Danen-van Oorschot AAAM, Zhang YH, Leliveld SR, et al. Importance of nuclear localization of apoptin for tumor-specific induction of apoptosis. J Biol Chem 2003;278:27729-36.
4. Zhang YH, Leliveld SR, Kooistra K, et al. Recombinant apoptin multimers kill tumor cells but are nontoxic and epitope-shielded in a normal-cell-specific fashion. Exp Cell Res 2003;289:36-46.
5. Poon IK, Oro C, Dias MM, Zhang JP, Jans DA. A tumor-cell specific nuclear targeting signal within chicken anaemia virus VP3/apoptin. J Virol 2004;79:1339-41.
6. Rohn JL, Zhang YH, Aalbers RIJM, et al. A tumor-specific kinase activity regulates the viral death protein Apoptin. J Biol Chem 2002;277:50820-7.
7. Baiker A, Maercker C, Piechaczek C. et al. Mitotic stability of an episomal vector containing a human scaffold/matrix-attached region is provided by association with nuclear matrix. Nat Cell Biol 2000;2:182-4.
8. Morgan M, Thorburn J, Pandolfi PP, Thorburn A. Nuclear and cytoplasmic shuttling of TRADD induces apoptosis via different mechanisms. J Cell Biol 2002;157:975-84.
9. Bird CH, Blink EJ, Hirst CE, et al. Nucleocytoplasmic distribution of the ovalbumin serpin PI-9 requires a non-conventional nuclear import pathway and the export factor Crm1. Mol Cell Biol 2001;21:5396-407.
10. Harley VR, Layfield S, Mitcheli CL, et al. Defective importin β recognition and nuclear import of the sex-determining factor SRY are associated with XY sex-reversing mutations. Proc Natl Acad Sci U S A 2003;100:7045-50.
11. 1 phase of the cell cycle. Cell 1991;67:293-302.
12. Kaelin WG Jr., Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell 1991;64:521-32.
13. Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 1981;23:175-82.
14. Guelen L, Paterson H, Gaken J, Meyers M, Farzaneh F, Tavassoli M. TAT-apoptin is efficiently delivered and induces apoptosis in cancer cells. Oncogene 2004;23:1153-65.
15. Kudo N, Wolff B, Sekimoto T, et al. Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1. Exp Cell Res 1998;242:540-7.
16. Engelhardt OG, Boutell C, Orr A, Ullrich E, Haller O, Everett RD. The homeodomain-interacting kinase PKM (HIPK-2) modifies ND10 through both its kinase domain and a SUMO-I interaction motif and alters the posttranslational modification of PML. Exp Cell Res 2003;283:36-50.
17. Wadia JS, Wagner MV, Ezhevsky SA, Dowdy SF. Apoptin/VP3 contains a concentration-dependent nuclear localization signal (NLS), not a tumorigenic selective NLS. J Virol 2004;78:6077-8.
18. Wang QM, Fan GC, Chen JZ, Chen HP, He FC. A putative NES mediates cytoplasmic localization of apoptin in normal cells. Acta Biochim Biophys Sin 2004; 36:817-23.
19. Jans DA, Xiao CY, Lam MHC. Nuclear targeting signal recognition: a key control point in nuclear transport? Bioessays 2000;22:532-44.
20. Brinkworth RI, Breinl RA, Kobe B. Structural basis and prediction of substrate specificity in protein serine/threonine kinases. Proc Natl Acad Sci U S A 2003;100:74-8.