A rapid test to monitor alloreactive responses in whole blood using real-time polymerase chain reaction

Transplantation

Volumn 80, Issue 3, 2005, Pages 410-413

  Zhou, Ling ^a   Toungouz, Michel ^a   Donckier, Vincent ^a   Andrien, Marc ^a   Troisi, Roberto ^b   De Hemptinne, Bernard ^b   Le Moine, Alain ^c   Dupont, Etienne ^a   Goldman, Michel ^a,c   Stordeur, Patrick ^a  

^a ERASME HOSPITAL   (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

^c UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

HLA DR; Interferon ; Interleukin 2; Mixed lymphocyte reaction; Real time polymerase chain reaction

Indexed keywords

CD3 ANTIGEN; GAMMA INTERFERON; HLA DR ANTIGEN; INTERLEUKIN 2; MESSENGER RNA;

ALLOIMMUNITY; ARTICLE; BLOOD; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HLA MATCHING; HUMAN; HUMAN CELL; IMMUNOLOGICAL TOLERANCE; IMMUNOSUPPRESSIVE TREATMENT; LIVER TRANSPLANTATION; MIXED LYMPHOCYTE REACTION; PATIENT MONITORING; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

ANTIBODY FORMATION; ANTIGENS, CD3; CYTOKINES; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELLS; HISTOCOMPATIBILITY TESTING; HLA-DR ANTIGENS; HUMANS; IMMUNOLOGIC TECHNIQUES; INTERFERON TYPE II; INTERLEUKIN-2; LEUKOCYTES, MONONUCLEAR; LIVER TRANSPLANTATION; LYMPHOCYTE CULTURE TEST, MIXED; PEPTIDES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; T-LYMPHOCYTES; TIME FACTORS; TRANSPLANTATION CHIMERA; TRANSPLANTATION TOLERANCE; TRANSPLANTATION, HOMOLOGOUS;

EID: 23744450295     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.tp.0000167818.78950.f2     Document Type: Article

References (13)

1. Mazariegos GV, Reyes J, Marino IR, et al. Weaning of immunosuppression in liver transplant recipients. Transplantation 1997; 63: 243.
2. Matthews JB, Ramos E, Bluestone JA. Clinical trials of transplant tolerance: slow but steady progress. Am J Transplant 2003; 3: 794.
3. Bouma GJ, Schanz U, Oudshoorn M, et al. A cell-saving non-radioactive limiting dilution analysis-assay for the combined determination of helper and cytotoxic T lymphocyte precursor frequencies. Bone Marrow Transplant 1996; 17: 19.
4. van der Mast BJ, van Besouw NM, de Kuiper P, et al. Pretransplant donor-specific helper T cell reactivity as a tool for tailoring the individual need for immunosuppression. Transplantation 2001; 72: 873.
5. Hornick PI, Brookes PA, Mason PD, et al. Optimizing a limiting dilution culture system for quantifying the frequency of interleukin-2-producing alloreactive T helper lymphocytes. Transplantation 1997; 64: 472.
6. Hernandez-Fuentes MP, Warrens AN, Lechler RJ. Immunologic monitoring. Immunol Rev 2003; 196: 247.
7. Stordeur P, Poulin L, Craciun L, et al. Cytokine mRNA quantification by real-time PCR. J Immunol Methods 2002;259: 55.
8. Erratum in J Immunol Methods 2002; 262: 229.
9. Stordeur P, Zhou L, Byl B, et al. Immune monitoring in whole blood using real time PCR. J Immunol Methods 2003; 276: 69.
10. Stordeur P, Zhou L, Goldman M. Analysis of spontaneous mRNA cytokine production in peripheral blood. J Immunol Methods 2002; 261: 195.
11. Moudgil A, Toyoda M, Galfayan K, Jordan SC. Selective expression of the interleukin-2 gene discriminates between the auto- and allo-mixed lymphocyte reaction. Transpl Immunol 1997; 5: 35.
12. Toungouz M, Denys C, deGroote D, et al. Optimal control of interferon-gamma and tumor necrosis factor-alpha by interleukin-10 produced in response to one HLA-DR mismatch during the primary mixed lymphocyte reaction. Transplantation 1996; 61: 497.
13. Donckier V, Troisi R, Toungouz M, et al. Donor stem cell infusion after non-myeloablative conditioning for tolerance induction to HLA mismatched adult living-donor liver graft. Transpl Immunol 2004; 13: 139.