A cell-saving non-radioactive limiting dilution analysis-assay for the combined determination of helper and cytotoxic T lymphocyte precursor frequencies

Bone Marrow Transplantation

Volumn 17, Issue 1, 1996, Pages 19-23

  Bouma, G J ^a   Schanz, U ^b   Oudshoorn, M ^a   Van Der Meer Prins, P M W ^a   Roelen, D L ^c   Van Rood, J J ^a   Claas, F H J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^c JOHN RADCLIFFE HOSPITAL   (United Kingdom)

Author keywords

Cytotoxic T lymphocyte precursor frequency; Helper T lymphocyte precursor frequency; Limiting dilution analysis; Non radioactive

Indexed keywords

ARTICLE; BONE MARROW TRANSPLANTATION; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; DILUTION; GRAFT VERSUS HOST REACTION; HELPER CELL; HUMAN; HUMAN CELL; LABORATORY TEST; LYMPHOCYTE COUNT; NORMAL HUMAN; PRIORITY JOURNAL; PROLYMPHOCYTE;

HEMATOPOIETIC STEM CELLS; HUMANS; LYMPHOCYTE COUNT; T-LYMPHOCYTES, CYTOTOXIC; T-LYMPHOCYTES, HELPER-INDUCER;

EID: 0030058953     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Hows JM, Yin JL, Marsh J et al. Histocompatible unrelated volunteer donors compared with HLA nonidentical family donors in marrow transplantation for aplastic anaemia and leukaemia. Blood 1986; 68: 1322-1328.
2. Irle C, D' Amaro J, Van Rood JJ. Bone marrow transplantation from other than non-identical siblings. Bone Marrow Transplant 1988; 3(suppl. 1): 37-38.
3. DeGast GC, Mickelson EM, Beatty PG et al. Mixed leukocyte culture reactivity and graft-versus-host disease in HLA-identical marrow transplantation for leukemia. Bone Marrow Transplant 1992; 9: 87-90.
4. Kaminuki E, Hows J, Man S et al. Prediction of graft-versus-host disease by frequency analysis of cytotoxic T cells after unrelated donor bone marrow transplantation. Transplantation 1989; 48- 608-613.
5. Roosnek E, Hogendijk S, Zawadynski S et al. The frequency of pretransplant donor cytotoxic T cell precursors with antihost specificity predicts survival of patients transplanted with bone marrow from donors other than HLA-identical siblings. Transplantation 1993; 56: 691-696.
6. Fussell ST, Donnellan M, Cooley MA, Farrell C. Cytotoxic T lymphocyte precursor frequency does not correlate with either the incidence or severity of graft-versus-host disease after matched unrelated donor bone marrow transplantation. Transplantation 1994; 57: 673-676.
7. Theobald M, Nierle T, Bunjes D et al. Host-specific interleukin-2-secreting donor T-cell precursors as predictors of acute graft-versus-host disease in bone marrow transplantation between HLA-identical siblings. New Engl J Med 1992; 327: 1613-1617.
8. Schwarer AP, Jiang YZ, Brookes PA et al. Frequency of antirecipient alloreactive helper T-cell precursors in donor blood and graft-versus-host disease after HLA-identical sibling bone marrow transplantation. Lancet 1993; 341: 203-205.
9. Schwarer AP, Jiang YZ, Deacock S et al. Comparison of helper and cytotoxic antirecipient T cell frequencies in unrelated bone marrow transplantation. Transplantation 1994; 58: 1198-1203.
10. Lantz O, Alard P, Chavanel G et al. EBV cell-lines (LCL) and E-cells as stimulator cells for limiting dilution analysis (LDA) of alloreactive IL-2 secreting cells (IL-2-SC) and cytotoxic precursors (CTLp). J Immunol Methods 1991; 137: 121-132.
11. Schanz U, Roelen DL, Bruning JW et al. The relative radioresistance of interleukin-2 production by human peripheral blood lymphocytes: consequences for the development of a new limiting dilution assay for the enumeration of helper T lymphocyte precursor frequencies. J Immunol Methods 1994; 169: 221-230.
12. Zhang L, Li SG, Vandekerckhove B et al. Analysis of cytotoxic T cell precursor frequencies against individual HLA-A and -B alloantigens. J Immunol Methods 1989; 121: 39-45.
13. Bouma GJ, Van der Meer-Prins PMW, Van Bree FPMJ et al. Determination of cytotoxic T-lymphocyte precursor frequencies using Europium labeling as a nonradioactive alternative to labeling with Chromium-51. Hum Immunol 1992; 35: 85-92.
14. Schreuder GMTh, Hendriks GFJ, D'Amaro J, Persijn GG. An eight-year study of HLA-typing proficiency in Eurotransplant. Tissue Antigens 1986; 27: 131-141.
15. Deacock S, Schwarer A, Batchelor R et al. A rapid limiting dilution assay for measuring frequencies of alloreactive, interleukin-2-producing T cells in humans. J Immunol Methods 1992; 147: 83-92.
16. Heeg K, Steeg C, Wagner H, High-dose irradiated splenic stimulator cells show no endogenous interleukin-2 production but stimulate clonally developing helper T cells to produce interleukin-2. J Immunol Methods 1988; 109: 185-191.
17. Lin Y, Langman R, Cohn M. The priming of cytotoxic T-cell precursors is strictly helper T cell-dependent. Scand J Immunol 1992; 35: 621-626.
18. Sharrock CEM, Kaminski E, Man S, Limiting dilution analysis of human T cells: a useful clinical tool. Immunol Today 1990; 11: 281-286.
19. Strijbosch LWG, Buurman WA, Does RJM et al. Limiting dilution assays: experimental design and statistical analysis. J Immunol Methods 1987; 97: 133-140.
20. Petrie A. Lecture Notes on Medical Statistics. Blackwell Scientific Publications: Oxford. 1987.
21. Irschick EU, Hladik F, Niederwieser D et al. Studies on the mechanism of tolerance or graft-versus-host-disease in allogeneic bone marrow recipients at the level of cytotoxic T-cell precursor frequencies. Blood 1992; 79: 1622-1628.
22. Bishop DK, Shelby J, Eichwald EJ. Mobilization of T lymphocytes followins cardiac transplantation. Transplantation 1992; 53: 849-857.
23. Deacock SJ, Lechler RI. Positive correlation of T cell sensitization with frequencies of alloreactive T helper cells in chronic renal failure patients. Transplantation 1992; 54: 338-343.
24. DeBruyne LA, Ensley RD, Olsen SL et al. Increased frequency of alloantigen-reactive helper T lymphocytes is associated with human cardiac allograft rejection. Transplantation 1993; 56: 722-727.
25. Zanker B, Jooss-Rüdiger J, Franz H-E et al. Evidence that functional deletion of donor-reactive T lymphocytes in kidney allograft recipients can occur at the level of cytotoxic T cells, Il-2 producing T cells, or both. Transplantation 1993; 56: 628-632.